Method of screening for microorganism having enhanced cellulose productivity

Patent metadata

Field	Value
Publication number	US-10253347-B2
Application number	US-201615228162-A
Country	US
Kind code	B2
Filing date	Aug 4, 2016
Priority date	Nov 19, 2015
Publication date	Apr 9, 2019
Grant date	Apr 9, 2019

Abstract

Provided is a method of screening for a microorganism having enhanced cellulose productivity.

First claim

What is claimed is:

1. A method of screening for a microorganism having enhanced cellulose productivity, the method comprising:
culturing mutated or recombinant cellulose-producing microorganisms to obtain a plurality of cultures containing the respective microorganisms and produced cellulose;
culturing one or more non-genetically modified and non-mutated cellulose-producing microorganisms to provide at least one respective control group;
measuring an optical density A of each respective control group and each culture of the one or more mutated or recombinant cellulose producing microorganisms, wherein the optical density A is an optical density at a first wavelength in a visible light range of about 380 nm to about 420 nm;
measuring an optical density B of each respective control group and at least one mutated or recombinant cellulose producing microorganism, wherein the optical density B is an optical density at a second wavelength in the visible light range of about 750 nm to about 800 nm;
obtaining a ratio of optical density A/optical density B with respect to cultures that have an optical density A being greater than that of said respective control group; and
selecting a mutated or recombinant microorganism that has a ratio of optical density A/optical density B greater than that of said respective control group as a microorganism having enhanced cellulose productivity.

2. The method of claim 1 , wherein the cellulose-producing microorganisms comprise a microorganism belonging to Gluconacetobacter sp.

3. The method of claim 1 , wherein the mutated microorganism is produced by random mutagenesis.

4. The method of claim 1 , wherein the microorganisms are cultured in the wells of a microplate.

5. The method of claim 1 , wherein the optical density B is measured only with respect to cultures in which the optical density A is greater than that of the respective control group.

6. The method of claim 1 , further comprising dispersing the produced cellulose in the culture prior to measuring the optical density A or the optical density B.

7. The method of claim 1 , wherein each of the optical density A and the optical density B is a mean value of three or more measurements with a deviation of 10% or less.

8. The method of claim 1 , wherein the optical density A and the optical density B are measured by a high throughput screening system.

9. The method of claim 1 , wherein the optical density A and the optical density B are measured during culturing.

10. The method of claim 1 , further comprising:
culturing the selected mutated or recombinant microorganism having a ratio of optical density A/optical density B higher than that of the control group to obtain a culture containing cellulose; and
measuring a content of the cellulose in the culture.

11. A method of producing cellulose, the method comprising:
selecting a mutated or recombinant microorganism according to the method of claim 1 ;
culturing the mutated or recombinant microorganism to produce cellulose; and recovering the cellulose from the culture.

12. The method of claim 11 , wherein the culturing is performed in flasks.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Byun Jongwon
• Chung Soonchun

Classifications

• C12P19/04

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title