Sulfated cellulose hydrate membrane, method for producing same, and use of the membrane as an adsorption membrane for a virus purification process

Patent metadata

Field	Value
Publication number	US-10253299-B2
Application number	US-201415029203-A
Country	US
Kind code	B2
Filing date	Sep 11, 2014
Priority date	Oct 14, 2013
Publication date	Apr 9, 2019
Grant date	Apr 9, 2019

Abstract

The present invention relates to a sulfated cellulose hydrate membrane, a method for the preparation thereof and the use of the membrane as adsorption membrane for the purification of viruses.

First claim

The invention claimed is:

1. A sulfated cellulose hydrate membrane which is a sheetlike adsorption membrane defining two outer main surfaces, the sulfated cellulose hydrate membrane comprising a crosslinked cellulose hydrate matrix with pores which extend from one outer main surface to an opposing outer main surface of the membrane, wherein the cellulose hydrate membrane has sulfate ligands on its inner surfaces and on its outer main surfaces for adsorptive substance separation wherein the degree of sulfation of the cellulose hydrate matrix is more than 20% by weight.

2. The sulfated cellulose hydrate membrane a claimed in claim 1 , wherein the mean pore size of the membrane is between 0.5 and 5.0 μm.

3. The sulfated cellulose hydrate membrane as claimed in claim 1 , wherein the degree of crosslinking of the cellulose hydrate matrix is 0.05 to 0.5.

4. A method for preparing a sulfated cellulose hydrate membrane as claimed in claim 1 , comprising the steps of:
providing a cellulose membrane with a pore size of 0.1 to 20 μm;
crosslinking the cellulose hydrate matrix using a crosslinker having at least two functional groups in the molecule which react with the hydroxyl groups of the cellulose hydrate matrix; and
sulfating the crosslinked cellulose hydrate matrix.

5. The method as claimed in claim 4 , wherein the crosslinker is selected from the group consisting of diepoxide compounds, diisocyanates, epichlorohydrin, epibromohydrin, dimethylurea, dimethylethyleneurea, dimethylchlorsilan, bis (2-hydroxyethylsulfone), divinylsulfone, alkylene dihalides, hydroxyalkylene dihalides and diglycidyl ethers or a mixture thereof.

6. The method as claimed in claim 4 , wherein 1, 4-butanediol diglycidyl ether or ethylene glycol diglycidyl ether is used as crosslinker.

7. The method as claimed in claim 4 , wherein the concentration of the crosslinker in the crosslinking solution of 10 to 30% by weights.

8. The method as claimed in claim 4 , wherein the sulfation is effected by reacting the crosslinked cellulose hydrate matrix having a Lewis base-SO 3 complex.

9. The method as claimed in claim 4 , wherein the sulfation is effected by reaction with a SO 3 -pyridine complex.

10. The method as claimed in claim 9 , wherein the concentration of SO 3 -pyridine-complex in the sulfation solution is 1 to 40% by weight.

11. The method as claimed in claim 4 , wherein the sulfation is effected at a temperature of 20 to 90° C.

12. A method of purifying viruses or virus fragments comprising:
preparing a sulfated cellulose hydrate membrane as claimed in claim 1 ; and
contacting the membrane with a solution containing the viruses or virus fragments.

13. The method of claim 12 wherein the viruses have a molecular mass of greater than 10 7 Da.

14. The method of claim 12 wherein the viruses are influenza viruses.

Assignees

• Sartorius Stedim Biotech Gmbh
• Sartorius Stedium Biotech Gmbh

Inventors

• Villain Louis
• Hörl Hans-Heinrich
• Brumm Christian

Classifications

• B01D2325/24

  Mechanical properties, e.g. strength · CPC title

• C12N2760/16051

  Methods of production or purification of viral material · CPC title

• B01D2323/30

  Cross-linking · CPC title

• B01D2325/12

  Adsorbents being present on the surface of the membranes or in the pores · CPC title

• B01D67/0093

  Chemical modification · CPC title