Phosphorescent nanoparticles and their uses in biosensing and bioimaging

Patent metadata

Field	Value
Publication number	US-10253255-B2
Application number	US-201615365031-A
Country	US
Kind code	B2
Filing date	Nov 30, 2016
Priority date	Nov 30, 2015
Publication date	Apr 9, 2019
Grant date	Apr 9, 2019

Abstract

Size-tunable phosphorescent particles may be formed through self-assembly of biocompatible linear polymers, such as chitosan and other linear polymers, that bear positive surface charges, through polyelectrolytic complexation to a polyanionic metal phosphor, such as polyanionic gold(I) phosphor (AuP). The phosphorescent hydrogel nanoparticles and thin films thereof are useful for imaging, sensing of biological molecules, detection of hypoxia, and light-emitting devices. The phosphorescent hydrogel particles can be formed from a variety of linear polymers by physical cross-linking using polyelectrolytic light-emitting species, without the need for the phosphorescent complex to be entrapped in an existing microsphere or nanosphere polymer particle.

First claim

What is claimed is:

1. Phosphorescent hydrogel nanoparticles, comprising:
polyanionic metal phosphors having light emitting properties, wherein the polyanionic metal phosphors are “PtP,” wherein “PtP” is Pt II (ptp) 2 , and wherein ptp is 3,5-bis(pyridyl)-1,2,4-triazolate); and
biocompatible linear polymers, wherein the biocompatible linear polymers are cross-linked and complexed through electrostatic interactions with the polyanionic metal phosphors, and wherein the polyanionic metal phosphors emit light when complexed with the biocompatible linear polymers.

2. The phosphorescent hydrogel nanoparticles of claim 1 , wherein the biocompatible linear polymers are chitosan polymers, poly-L-lysine (PLL), poly-ethyleneimine (PEI), poly-diallyldimethylammonium chloride (PDADMAC), polyacrylo nitrile (PAN), polyacrylic acid (PAA), or a combination thereof.

3. The phosphorescent hydrogel nanoparticles of claim 1 , wherein the biocompatible linear polymers are chitosan polymers.

4. Phosphorescent hydrogel nanoparticles, comprising:
polyanionic metal phosphors having light emitting properties, wherein the polyanionic metal phosphors are “Pt-POP,” wherein “Pt-POP” is M 4 [Pt 2 (P 2 O 5 H 2 ) 4 ].2H 2 P, and wherein P 2 O 5 H 2 is dihydrotetrakis(pyrophosphite) and M is potassium or sodium; and
biocompatible linear polymers, wherein the biocompatible linear polymers are cross-linked and complexed through electrostatic interactions with the polyanionic metal phosphors, and wherein the polyanionic metal phosphors emit light when complexed with the biocompatible linear polymers.

5. The phosphorescent hydrogel nanoparticles of claim 4 , wherein the biocompatible linear polymers are chitosan polymers, poly-L-lysine (PLL), poly-ethyleneimine (PEI), poly-diallyldimethylammonium chloride (PDADMAC), polyacrylo nitrile (PAN), polyacrylic acid (PAA), or a combination thereof.

6. The phosphorescent hydrogel nanoparticles of claim 4 , wherein the biocompatible linear polymers are chitosan polymers.

Assignees

• Univ North Texas

Inventors

• Omary Mohammad A
• Marpu Sreekar

Classifications

• C09K11/70

  containing phosphorus · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• B82Y20/00

  Nanooptics, e.g. quantum optics or photonic crystals · CPC title

• B82Y30/00

  Nanotechnology for materials or surface science, e.g. nanocomposites · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title