Self-assembling protein nanostructures

Patent metadata

Field	Value
Publication number	US-10248758-B2
Application number	US-201414759308-A
Country	US
Kind code	B2
Filing date	Feb 7, 2014
Priority date	Feb 7, 2013
Publication date	Apr 2, 2019
Grant date	Apr 2, 2019

Abstract

Synthetic nanostructures, proteins that are useful, for example, in making synthetic nanostructures, and methods for designing such synthetic nanostructures are disclosed herein.

First claim

The invention claimed is:

1. An isolated nanostructure, comprising
(a) a plurality of first proteins that self-interact to form a first multimeric substructure comprising at least one axis of rotational symmetry; and
(b) a plurality of second proteins that self-interact to form a second multimeric substructure comprising at least one axis of rotational symmetry;
wherein multiple copies of the first multimeric substructure and the second multimeric substructure interact with each other at one or more symmetrically repeated, non-natural, non-covalent protein-protein interfaces that orient the first multimeric substructures and the second multimeric substructures such that their symmetry axes are aligned with symmetry axes of the same kind in a designated mathematical symmetry group;
wherein the first protein and the second protein comprise proteins selected from the following pairs of first and second proteins:
(a) T32-28A (SEQ ID NO: 11) and T32-28B (SEQ ID NO: 12);
(b) T33-09A (SEQ ID NO: 13) and T33-09B (SEQ ID NO: 14);
(c) T33-15A (SEQ ID NO: 15) and T33-15B (SEQ ID NO: 16);
(d) T33-21A (SEQ ID NO: 17) and T33-21B (SEQ ID NO: 18);
(e) T33-28A (SEQ ID NO: 19) and T33-28B (SEQ ID NO: 20);
(f) T32-28A (SEQ ID NO: 21) and T32-28B (SEQ ID NO: 22);
(g) T33-09A (SEQ ID NO: 23) and T33-09B (SEQ ID NO: 24);
(h) T33-15A (SEQ ID NO: 25) and T33-15B (SEQ ID NO: 26);
(i) T33-21A (SEQ ID NO: 27) and T33-21B (SEQ ID NO: 28);
(i) T33-28A (SEQ ID NO: 29) and T33-28B (SEQ ID NO: 30);
(k) T32-28A (SEQ ID NO: 31) and T32-28B (SEQ ID NO: 32);
(l) T33-09A (SEQ ID NO: 33) and T33-09B (SEQ ID NO: 34);
(m) T33-15A (SEQ ID NO: 35) and T33-15B (SEQ ID NO: 36);
(n) T33-21A (SEQ ID NO: 37) and T33-21B (SEQ ID NO: 38); and
(o) T33-28A (SEQ ID NO: 39) and T33-28B (SEQ ID NO: 40).

2. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T32-28A (SEQ ID NO: 11) and T32-28B (SEQ ID NO: 12).

3. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-09A (SEQ ID NO: 13) and T33-09B (SEQ ID NO: 14).

4. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-15A (SEQ ID NO: 15) and T33-15B (SEQ ID NO: 16).

5. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-21A (SEQ ID NO: 17) and T33-21B (SEQ ID NO: 18).

6. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-28A (SEQ ID NO: 19) and T33-28B (SEQ ID NO: 20).

7. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T32-28A (SEQ ID NO: 21) and T32-28B (SEQ ID NO: 22).

8. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-09A (SEQ ID NO: 23) and T33-09B (SEQ ID NO: 24).

9. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-15A (SEQ ID NO: 25) and T33-15B (SEQ ID NO: 26).

10. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-21A (SEQ ID NO: 27) and T33-21B (SEQ ID NO: 28).

11. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-28A (SEQ ID NO: 29) and T33-28B (SEQ ID NO: 30).

12. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T32-28A (SEQ ID NO: 31) and T32-28B (SEQ ID NO: 32).

13. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-09A (SEQ ID NO: 33) and T33-09B (SEQ ID NO: 34).

14. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-15A (SEQ ID NO: 35) and T33-15B (SEQ ID NO: 36).

15. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-21A (SEQ ID NO: 37) and T33-21B (SEQ ID NO: 38).

16. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-28A (SEQ ID NO: 39) and T33-28B (SEQ ID NO: 40).

17. An isolated nanostructure, comprising
(a) a plurality of first proteins that self-interact to form a first multimeric substructure comprising at least one axis of rotational symmetry; and
(b) a plurality of second proteins that self-interact to form a second multimeric substructure comprising at least one axis of rotational symmetry;
wherein multiple copies of the first multimeric substructure and the second multimeric substructure interact with each other at one or more symmetrically repeated, non-natural, non-covalent protein-protein interferences that orient the first multimeric substructures and the second multimeric substructures such that their symmetry axes are aligned with symmetry axes of the same kind in a designated mathematical symmetry group;
wherein the first protein and the second protein comprise proteins selected from the following pairs of first and second proteins:
(a) SEQ ID NOs: 1 and 2;
(b) SEQ ID NOs: 3 and 4;
(c) SEQ ID NOs: 5 and 6;
(d) SEQ ID NOs: 7 and 8; and
(e) SEQ ID NOs: 9 and 10.

18. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 1-2.

19. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 3-4.

20. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 5-6.

21. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 7-8.

22. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 9-10.

Assignees

• Univ Washington Through Its Center For Commercialization

Inventors

• Baker David
• King Neil
• Bale Jacob
• Sheffler William

Classifications

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• C07K14/00

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• C07K2319/735

  containing a domain for self-assembly, e.g. a viral coat protein (includes phage display) · CPC title

• C07K14/195

  from bacteria · CPC title

• G01N2570/00

  Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes · CPC title