Portable efficient magnetic solid phase extraction device and extraction method thereof

Patent metadata

Field	Value
Publication number	US-10247642-B2
Application number	US-201715619456-A
Country	US
Kind code	B2
Filing date	Jun 10, 2017
Priority date	Aug 29, 2016
Publication date	Apr 2, 2019
Grant date	Apr 2, 2019

Abstract

A device comprises a contact reaction chamber, a circulating water inlet, a clear water basin, a circulating pump, a circulating water outlet, a solid phase extractant collecting tank, a magnetic holder, an electromagnet, a solid-liquid separation area, a drain valve, a wall sprinkling water inlet, and a wall sprinkling pipe, wherein, the contact reaction chamber is in a conical shape, and utilizes hydraulic power to perform stir to ensure no dead corner exists during contact stir; the obconical solid-liquid separation area increases the action area between a magnetic solid phase extractant and the electromagnet; the solid phase extractant collecting tank is in a downwards protruding dish shape to prevent the solid phase extractant from losing.

First claim

What is claimed is:

1. A portable efficient magnetic solid phase extraction device, characterized in that: the device mainly comprises a contact reaction chamber ( 1 ), a circulating water inlet ( 2 ), a clear water basin ( 3 ), a three-way valve c( 4 ), a water delivery pipe a- 1 ( 5 - 1 ), a water delivery pipe a- 2 ( 5 - 2 ), a circulating pump ( 6 ), a circulating water outlet ( 7 ), a solid phase extractant collecting tank ( 8 ), a magnetic holder ( 9 ), an electromagnet ( 10 ), a solid-liquid separation area ( 11 ), a water outlet ( 12 ), a three-way valve d( 13 ), a water delivery pipe b- 1 ( 14 - 1 ), a water delivery pipe b- 2 ( 14 - 2 ), a drain pipe ( 15 ), a drain valve ( 16 ), a wall sprinkling water inlet ( 17 ), and a wall sprinkling pipe ( 18 ),
wherein, the circulating water inlet ( 2 ) and the circulating water outlet ( 7 ) are respectively located at the upper part and lower part of the contact reaction chamber ( 1 ); the circulating water inlet ( 2 ) is connected to the three-way valve c( 4 ) via the water delivery pipe a- 1 ( 5 - 1 ); the circulating water outlet ( 7 ) is connected to the three-way valve c( 4 ) via a pipe; the clear water basin ( 3 ) is connected to the pipe between the circulating water outlet ( 7 ) and the three-way valve c( 4 ) via a pipe; the lower part of the contact reaction chamber ( 1 ) is connected to the solid-liquid separation area ( 11 ) via the water outlet ( 12 ); the solid phase extractant collecting tank ( 8 ), the electromagnet ( 10 ), and the magnetic holder ( 9 ) are sequentially disposed at the lower part of the solid-liquid separation area ( 11 );
the solid phase extractant collecting tank ( 8 ) is connected to the drain valve ( 16 ) via a pipe; the drain valve ( 16 ) is connected to the drain pipe ( 15 ); the sprinkling pipe ( 18 ) is located at the upper part inside the contact reaction chamber ( 1 ), and is connected to the wall sprinkling water inlet ( 17 ); the wall sprinkling water inlet ( 17 ) is connected to the three-way valve d( 13 ) via the water delivery pipe b- 1 ( 14 - 1 ); the three-way valve d( 13 ) is connected to the pipe between the solid phase extractant collecting tank ( 8 ) and the drain valve ( 16 ) via a pipe; the three-way valve d( 13 ) is connected to the circulating pump ( 6 ) via the water delivery pipe b- 2 ( 14 - 2 ); the circulating pump ( 6 ) is connected to three-way valve c( 4 ) via the water delivery pipe a- 2 ( 5 - 2 ); and the entire device is automatically controlled by a circuit.

2. The portable efficient magnetic solid phase extraction device as claimed in claim 1 , characterized in that: the contact reaction chamber ( 1 ) is made from stainless steel, and is in a conical shape; the circulating water inlet ( 2 ) is located 5-8 cm away from the upper edge of the contact reaction chamber ( 1 ); the diameters of the circulating water outlet ( 7 ) and the circulating water inlet ( 2 ) are 2-5 cm.

3. The portable efficient magnetic solid phase extraction device as claimed in claim 1 , characterized in that: the circulating pump ( 6 ) is located at the lower part of the device, and has a pump discharge rate of 16 L/min and a lift of 6 m; water enters the water delivery pipe a- 2 ( 5 - 2 ) from the circulating water inlet ( 2 ), flows through the circulating pump ( 6 ), and is sprayed upwards from the circulating water outlet ( 7 ) to perform hydraulic stir for 5-30 min.

4. The portable efficient magnetic solid phase extraction device as claimed in claim 1 , characterized in that: the solid phase extractant collecting tank ( 8 ), the magnetic holder ( 9 ), the electromagnet ( 10 ), and the solid-liquid separation area ( 11 ) are located 5-10 cm under the water outlet ( 12 ) of the contact reaction chamber ( 1 ); the magnetic holder ( 9 ) is located undermost; the electromagnet ( 10 ) and the solid phase extractant collecting tank ( 8 ) are sequentially disposed on the magnetic holder ( 9 ), the three of which closely press against each other.

5. The portable efficient magnetic solid phase extraction device as claimed in claim 1 , characterized in that: the solid-liquid separation area ( 11 ) is in an obconical shape, and has a diameter of 8-12 cm.

6. The portable efficient magnetic solid phase extraction device as claimed in claim 1 , characterized in that: the electromagnet ( 10 ) is in a circular shape, and has a diameter of 8-12 cm and a magnetic field strength of 300-1000 Gs.

7. The portable efficient magnetic solid phase extraction device as claimed in claim 1 , characterized in that: the solid phase extractant collecting tank ( 8 ) is in a downwards protruding dish shape; and the left side of the solid phase extractant collecting tank ( 8 ) is higher than the right side with a height difference of 1-2 cm.

8. The portable efficient magnetic solid phase extraction device as claimed in claim 1 , characterized in that: an auxiliary wall sprinkling water inlet ( 17 ) is located 2-4 cm away from the upper edge of the contact reaction chamber ( 1 ); the wall sprinkling pipe ( 18 ) is located on a circumference as high as the wall sprinkling water inlet ( 17 ); and 6-10 number of sprinkler heads are uniformly distributed on the wall sprinkling pipe.

9. The portable efficient magnetic solid phase extraction device as claimed in claim 1 , characterized in that: the clear water basin ( 3 ) is located in front of the three-way valve c( 4 ), and has a volume of 100-300 mL.

10. An extraction method of the portable efficient magnetic solid phase extraction device as claimed in claim 1 , characterized in that: the method adopts the portable efficient magnetic solid phase extraction device, and comprises the steps of:
(a) placing a to-be-treated water sample and a magnetic solid phase extractant in the contact reaction chamber ( 1 ), respectively;
(b) setting the operating time and drain time of the circulating pump ( 6 ), switching on a power supply to start up the circulating pump ( 6 ), and enabling mixed solution to flow through the three-way valve c( 4 ) to fully and completely mix the magnetic solid phase extractant with the to-be-treated water sample, respectively;
(c) closing the three-way valve c( 4 ), opening the water outlet ( 12 ) and the three-way valve d( 13 ), at the stage of collecting the solid phase extractant, in the solid-liquid separation area ( 11 ), collecting the solid phase extractant into the solid phase extractant collecting tank ( 8 ) under the action of the electromagnet ( 10 ), enabling the treated water sample to flow through the three-way valve d( 13 ) and arrive at the wall sprinkling pipe ( 18 ), and flushing a small amount of remaining solid phase extractant into the solid phase extractant collecting tank ( 8 );
(d) closing the three-way valve d( 13 ), opening the drain valve ( 16 ) to discharge the water sample, simultaneously opening the clear water basin ( 3 ) and the three-way valve c( 4 ), and utilizing clear water to flush the residual solid phase extractant in the pipe into the solid phase extractant collecting tank ( 8 ) for collection; and
(e) after the collection is completed, removing the solid phase extractant collecting tank ( 8 ), taking out and storing the magnetic solid phase extractant for subsequent elution.

Assignees

• Nanjing University Of Technology

Inventors

• Zhou Qing
• Wang Wei
• Li Aimin
• Zhao Qingqing
• Wang Minglu
• Gao Rui
• Ma Xiaowen
• Shi Peng
• Chen Xun

Classifications

• B03C2201/18

  Magnetic separation whereby the particles are suspended in a liquid · CPC title

• G01N1/18 Primary

  with provision for splitting samples into portions (G01N1/12, G01N1/14 take precedence; fraction-collection apparatus for chromatography B01D15/08) · CPC title

• B03C1/288

  disposed at the outer circumference of a recipient · CPC title

• G01N1/405 Primary

  by adsorption or absorption · CPC title

• B03C1/0335

  using coils · CPC title