Crop resistance to nematodes

Patent metadata

Field	Value
Publication number	US-10246722-B2
Application number	US-201214232041-A
Country	US
Kind code	B2
Filing date	Jul 13, 2012
Priority date	Jul 13, 2011
Publication date	Apr 2, 2019
Grant date	Apr 2, 2019

Abstract

Methods of inhibiting plant parasitic nematodes, methods of obtaining transgenic plants useful for inhibiting such nematodes, and transgenic plants that are resistant to plant parasitic nematodes through inhibition of plant nematode CLAVATA3/ESR (CLE) peptide receptor genes are provided. Methods for expressing genes at plant parasitic nematode feeding sites with plant nematode CLE peptide receptor gene promoters are also provided, along with nematode CLE peptide receptor gene promoters that are useful for expressing genes in nematode feeding sites as well as transgenic plants and nematode resistant transgenic plants comprising the promoters.

First claim

What is claimed is:

1. A method for inhibiting plant parasitic nematode damage to a soybean plant, said method comprising:
(1) reducing the expression of an endogenous plant RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2)-like gene by expressing in said soybean plant RNAi to silence said RPK2-like gene, wherein said RPK2-like gene is set forth in SEQ ID NO:17, SEQ ID NO: 18, SEQ ID NO: 20 or SEQ ID NO: 21; and
(2) growing said soybean plant expressing said RNAi,
thereby inhibiting plant parasitic nematode damage to said soybean plant.

2. The method of claim 1 , further comprising reducing the expression of an endogenous CLAVATA2 (CLV2) gene by introducing into said soybean plant one or more non-natural mutations in said CLV2 gene, or by expressing in said soybean plant RNAi to silence said CLV2 gene.

3. The method of claim 1 , further comprising the step of harvesting a product of said soybean plant.

4. The method of claim 3 , wherein said product is a leaf, stem, flower, seed, root, or tuber.

5. The method of claim 3 , wherein the yield and/or quality of said product is increased relative to a control plant not expressing said RNAi.

6. The method of claim 1 , wherein said plant parasitic nematode is a cyst nematode.

7. The method of claim 6 , wherein said cyst nematode is a Heterodera spp.

8. The method of claim 6 , wherein said plant parasitic nematode is Heterodera glycines.

9. A genetically modified nematode resistant soybean plant produced by the method of claim 1 , wherein said soybean plant expresses said RNAi.

Assignees

• Mitchum Melissa G
• Replogle Amy
• Wang Jianying
• Wang Xiaohong
• Chen Shiyan
• Lang Ping
• Univ Missouri
• Univ Cornell
• Us Agriculture

Inventors

• Mitchum Melissa G
• Replogle Amy
• Wang Jianying
• Wang Xiaohong
• Chen Shiyan
• Lang Ping

Classifications

• C12N15/8239

  pathogen inducible · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8285 Primary

  for nematode resistance · CPC title

• Y02A40/164

  Cross-Sectional Technologies · mapped topic

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title