Method to perform a measurement of an analyte in a sample using an automatic analyzer

Patent metadata

Field	Value
Publication number	US-10241124-B2
Application number	US-201615092806-A
Country	US
Kind code	B2
Filing date	Apr 7, 2016
Priority date	Oct 8, 2013
Publication date	Mar 26, 2019
Grant date	Mar 26, 2019

Abstract

A method of performing a measurement of an analyte in a sample using an automatic analyzer is provided. The automatic analyzer comprises: a cartridge for dispensing a fluid, a measurement unit for performing the measurement, a sample holder for receiving the sample, and a pump for pumping the fluid out of the cartridge and into the sample holder. The cartridge comprises: a rigid portion, a flexible bladder, and an outlet. The rigid portion comprises an opening, which is connected to an inner cavity. The flexible bladder seals the opening to form a fluid chamber from the inner cavity. The fluid chamber is at least partially filled with the fluid. The pump is connected to the outlet. The method comprises: placing the sample into the sample holder, controlling the pumping of the fluid from the cartridge into the sample holder, and performing the measurement of the analyte using the measurement unit.

First claim

What is claimed is:

1. A method of performing a measurement of an analyte in a sample using an automatic analyzer, wherein the automatic analyzer comprises:
a cartridge for dispensing a fluid,
a measurement unit for performing the measurement,
a sample holder for receiving the sample, and
a pump for pumping the fluid out of the cartridge and into the sample holder;

wherein the cartridge comprises:
a rigid portion,
a flexible bladder, and
an outlet; wherein the outlet is attached to the rigid portion; wherein the rigid portion comprises an inner cavity; wherein the outlet is connected to the inner cavity;
wherein the rigid portion comprises an opening; wherein the opening is connected to the inner cavity; wherein the flexible bladder seals the opening to form a fluid chamber from the inner cavity; wherein the fluid chamber is at least partially filled with the fluid; and

wherein the pump is connected to the outlet; and
wherein the method comprises:
placing the sample into the sample holder;
controlling the pumping of the fluid from the cartridge into the sample holder; and
performing the measurement of the analyte using the measurement unit.

2. The method of claim 1 , wherein the flexible bladder has an exterior surface, and wherein the exterior surface is exposed to constant pressure during pumping of the fluid from the cartridge into the sample holder.

3. An automatic analyzer for performing a measurement of an analyte in a sample, wherein the automatic analyzer comprises:
a cartridge for dispensing a fluid,
a measurement unit for performing the measurement,
a sample holder for receiving the sample,
a pump for pumping the fluid out of the cartridge to the sample holder,
a memory for storing machine executable instructions, and
a processor for controlling the automatic analyzer; wherein the cartridge comprises:
a rigid portion,
a flexible bladder, and

an outlet; wherein the outlet is attached to the rigid portion; wherein the rigid portion comprises an inner cavity; wherein the outlet is connected to the inner cavity; wherein the rigid portion comprises an opening; wherein the opening is connected to the inner cavity; wherein the flexible bladder seals the opening to form a fluid chamber from the inner cavity; wherein the fluid chamber is at least partially filled with the fluid; wherein the pump is connected to the outlet; and wherein execution of the instructions causes the processor to:
control the pump to pump the fluid from the cartridge to the sample holder; and
control the measurement unit to perform the measurement of the analyte.

4. The automatic analyzer of claim 3 , wherein when the fluid chamber is filled with the fluid the flexible bladder is configured to expand out of the inner cavity to form a bladder volume; and wherein the bladder volume is defined by the opening and the flexible bladder.

5. The automatic analyzer of claim 4 , wherein the flexible bladder is inelastic, and wherein when the fluid chamber is fully filled with the fluid the bladder volume is approximately equal to the volume of the inner cavity.

6. The automatic analyzer of claim 4 , wherein the flexible bladder is elastic.

7. The automatic analyzer of claim 4 , wherein the inner cavity has an inner cavity surface, and wherein when the fluid chamber is in an empty condition the flexible bladder is configured to cover the inner cavity surface.

8. The automatic analyzer of claim 4 , wherein the cartridge further comprises a cap, wherein the cap forms a cap cavity, wherein the cap cavity is positioned about the opening, and wherein the cap cavity is configured to receive the flexible bladder when filled with the fluid.

9. The automatic analyzer of claim 8 , wherein the cap is attached to the rigid portion to seal the cap cavity, and wherein the cap comprises a gas inlet that's configured for pressurizing and/or ventilating the cap cavity.

10. The automatic analyzer of claim 3 , wherein the cartridge comprises a fluid inlet on the rigid portion, and wherein the fluid inlet is sealable with a seal.

11. The automatic analyzer of claim 3 , wherein the outlet is circular, wherein the outlet has an outlet diameter, wherein the cavity is symmetric about an axis of symmetry, wherein the cavity has a cavity radius about the axis of symmetry, wherein the axis of symmetry passes through the opening and the outlet, and wherein the cavity radius is monotonically decreasing from the opening to the outlet.

12. The automatic analyzer of claim 3 , wherein the cartridge further comprises a valve for sealing and unsealing the outlet and/or wherein the pump comprises a nozzle for dispensing the fluid.

13. The automatic analyzer of claim 3 , wherein the cartridge is detachable from the pump.

14. The automatic analyzer of claim 3 , wherein the pump has an inlet, and wherein the automatic analyzer further comprises a sealed coupling for attaching the pump inlet to the cartridge outlet.

15. The automatic analyzer of claim 3 , wherein the measurement unit is any one of the following: a test strip analyzer, a urine test strip analyzer, a fluorescence spectrometer, a photospectrometer, a spectrometer, a scattered light spectrometer, a Chemiluminescence system, and electrochemiluminescense (ECL) measurement system, mass spectrometer, cell counter, optical imaging system, a staining system, a tissue diagnostics system, a turbidimetric measurement system, or a nephelometric measurement system.

16. The automatic analyzer of claim 3 , wherein the automatic analyzer comprises a cartridge assembly, and wherein the cartridge assembly comprises the cartridge and the pump.

17. The automatic analyzer of claim 16 , wherein the pump is permanently attached to the cartridge.

18. The automatic analyzer of claim 3 , wherein the automatic analyzer further comprises multiple cartridges, wherein the automatic analyzer further comprises a translation mechanism for moving the pump between the multiple cartridges, wherein the translation mechanism is configured to attach the pump to any one of the multiple cartridges, and wherein the translation mechanism is configured to detach the pump from any one of the multiple cartridges.

19. The automatic analyzer of claim 3 , wherein the automatic analyzer further comprises multiple cartridges, wherein the automatic analyzer comprises multiple pumps, wherein the automatic analyzer further comprises a translation mechanism for either moving the sample holder to each of the multiple cartridges or for moving each of the multiple cartridges to the sample holder.

20. A cartridge assembly, wherein the cartridge assembly comprises:
a cartridge for dispensing a fluid through an outlet, and
a pump for pumping the fluid in the cartridge through the outlet;

wherein the cartridge comprises:
a rigid portion, and
a flexible bladder;

wherein the outlet is attached to the rigid portion; wherein the rigid portion comprises an inner cavity; wherein the outlet is connected to the inner cavity; wherein the rigid portion comprises an opening; wherein the opening is connected to the inner cavity; wherein the flexible bladder seals the opening to form a fluid chamber from the inner cavity; wherein the pump is connected to the outlet; wherein when the fluid chamber is filled with the fluid the flexible bladder is configured to expand out of the inner cavity to form a bladder volume; and wherein the bladder volume is defined by the opening and the flexible bladder.

Assignees

• Roche Diagnostics Operations Inc

Inventors

• Augstein Manfred
• Losleben Nadine
• Brueckner Thorsten
• Boehm Christoph
• Spinke Juergen

Classifications

• B01L2300/123

  Flexible; Elastomeric · CPC title

• G01F11/086

  using an auxiliary pressure to cooperate with the diaphragm or bellows · CPC title

• B01L2300/042

  Caps; Plugs · CPC title

• B01L2200/04

  Exchange or ejection of cartridges, containers or reservoirs · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title