Method of stabilizing molecules without refrigeration using water soluble polymers and applications thereof in performing chemical reactions

Patent metadata

Field	Value
Publication number	US-10241109-B2
Application number	US-201415034914-A
Country	US
Kind code	B2
Filing date	Nov 10, 2014
Priority date	Nov 8, 2013
Publication date	Mar 26, 2019
Grant date	Mar 26, 2019

Abstract

The present application is directed to methods of performing chemical reactions, including multi-step chemical reactions in which two or more of the reagents in the chemical reaction are incorporated or entrapped in a solid polymeric structure comprising pullulan. In certain embodiments, the chemical reaction or multi-step reaction serves as a sensor. Accordingly the present application is also directed to sensors for performing the methods of the application. In certain embodiments, at least one of the reagents is a biomolecule and the sensor is a biosensor. In certain other embodiments, the solid polymeric structure comprising pullulan and the reagents for performing a chemical reaction form a convenient device for performing a chemical reaction.

First claim

The invention claimed is:

1. A method of performing a single step or multi-step chemical reaction comprising:
a) combining two or more reagents for the reaction, either separately or together, with an aqueous pullulan solution to provide reagent pullulan solutions or a reagent pullulan solution, respectively;
b) drying the reagent pullulan solutions or the reagent pullulan solution to provide solid polymeric structures or a solid polymeric structure, respectively; and
c) if the two or more reagents are in separate solid polymeric structures in b), then treating the solid polymeric structures under conditions to dissolve the solid polymeric structures and for the reagents to interact in a chemical reaction; or
d) if the two or more reagents are together in the solid polymeric structure in b), then treating the solid polymeric structure under conditions to dissolve the solid polymeric structure and for the reagents to interact in a chemical reaction,
wherein the solid polymeric structures are or the solid polymeric structure is the components(s) of a sensor comprising two or more reagents for performing a chemical reaction entrapped in the same solid polymeric structure or in different solid polymeric structures wherein the solid polymeric structures are prepared from an aqueous solution comprising about 5% (w/v) to about 25% (w/v) of pullulan and at least one of the two or more reagents is an enzyme.

2. The method of claim 1 , wherein the conditions to dissolve the solid polymeric structure(s) comprise contacting the solid polymeric structure(s) with water or an aqueous buffer.

3. The method of claim 1 , wherein, the two or more reagents are in separate solid polymeric structures and the solid polymeric structures are stacked in layers on top of each other.

4. The method of claim 3 , wherein the layers are stacked in an order that corresponds to an order required to perform the chemical reaction.

5. The method of claim 1 , wherein the two or more reagents are in separate solid polymeric structures and the solid polymeric structures are in the shape of a pill, tablet or capsule, with each separate polymeric structure forming separate layers surrounding each other and the outer layer of the pill, tablet or capsule comprises reagents that must react first in the chemical reaction and the remaining layers are arranged internally to the outer layer in an order that corresponds to an order required to perform the chemical reaction.

6. The method of claim 3 , wherein timing of dissolution of the layers is controlled to allow sufficient reaction time for each step of the chemical reaction.

7. The method of claim 6 , wherein the timing is controlled by one or more of thickness of the layers and pullulan concentration.

8. The method of claim 1 , wherein the two or more reagents are in the same solid polymeric structure.

9. The method of claim 1 , wherein the two or more reagents are in separate solid polymeric structures and the solid polymeric structures are each dissolved in a single water or buffer solution to release the reagents for interaction in the chemical reaction.

10. A sensor comprising two or more reagents for performing a chemical reaction entrapped in the same solid polymeric structure or in different solid polymeric structures wherein the solid polymeric structures are prepared from an aqueous solution comprising about 5% (w/v) to about 25% (w/v) of pullulan and at least one of the two or more reagents is an enzyme.

11. The sensor of claim 10 , wherein the two or more reagents are entrapped in separate solid polymeric structures and the solid polymeric structures are stacked in layers on top of each other.

12. The sensor of claim 10 , wherein the two or more reagents are entrapped in separate solid polymeric structures and the solid polymeric structures are in the shape of a pill, tablet or capsule, with separate polymeric structures forming separate layers surrounding each other wherein a layer on the outside of the pill, tablet or capsule comprises reagents that must react first in the chemical reaction and the remaining layers are arranged internally to the outside layer in an order that corresponds to an order required to perform the chemical reaction.

13. The sensor of claim 10 , wherein the two or more reagents are entrapped in separate polymeric structures and the two or more reagents that are in separate polymeric structures comprise an enzyme and a substrate for the enzyme.

14. The sensor of claim 10 , wherein the two or more reagents are entrapped in the same solid polymeric structure.

15. The sensor of claim 10 , wherein one or more of the solid polymeric structures are cast onto a substrate comprising at least one of the two or more reagents for the chemical reaction.

16. The sensor of claim 10 , wherein the enzyme is DNA polymerases, restriction enzymes, DNA ligases, RNA ligases, luciferase, DNases, RNases, acetylcholine esterase, β-glucuronidase, β-galactosidase or lactate dehydrogenase.

17. The sensor of claim 10 , wherein at least one of the two or more reagents is an inorganic molecule selected from one or more of an inorganic acid, an inorganic base, an alkaline earth metal carbonate, an alkaline earth metal sulfate, an alkali metal carbonate, an alkali metal sulfate and a metal complex.

18. The sensor of claim 13 , wherein the substrate for the enzyme is 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-GLUC), 5-bromo-4-chloro-3-indolyl-R-D-galactopyranoside (X-GAL), 5-bromo-3-indolyl β-D-galactopyranoside (Bluo-Gla), 5-bromo-6-chloro-3-indolyl β-D-galactopryaniside (Magenta-Gal), 6-chloro-3-indolyl β-D-galactopyranoside (Salmon-Gal), 2-nitrophenyl β-D-galactopyranoside (ONPG) or 4-nitro β-D-galactopyranoside (PNPG).

19. The sensor of claim 10 , wherein the solid polymeric structures are prepared from an aqueous solution comprising about 10% (w/v) to about 20% (w/v) of pullulan.

20. The sensor of claim 10 , wherein the two or more reagents that are entrapped in different solid polymeric structures comprise acetylcholine esterase (AChE) and indoxyl acetate.

21. The sensor of claim 10 , wherein the two or more reagents in the same solid polymeric structure comprise reagents for detection of adenosine triphosphate (ATP).

22. The sensor of claim 10 , wherein the amount of the two or more reagents is premeasured in an amount needed to perform the chemical reaction.

23. The sensor of claim 10 , wherein the chemical reaction produces a product that is detectable.

24. The sensor of claim 23 , wherein the product is detectable using colorimetry, optical spectrometry and/or fluorescence.

25. The sensor of claim 24 , wherein the two or more reagents in the same solid polymeric structure comprise reagents for detection of adenosine triphosphate (ATP).

26. The sensor of claim 25 , wherein the reagents for ATP detection comprise luciferin and luciferase.

27. The sensor of claim 25 , wherein the reagents for ATP detection comprise luciferin, luciferase, coenzyme A (CoA), dithiothreitol (DTT), a chelating reagent, MgCO 3 and MgSO 4 .

Assignees

• Univ Mcmaster

Inventors

• Filipe Carlos
• Brennan John D
• Pelton Robert
• Jahanshahi-Anbuhi Sana
• Li Yingfu

Classifications

• G01N2333/922

  Ribonucleases (RNAses); Deoxyribonucleases (DNAses) · CPC title

• G01N33/548

  Carbohydrates, e.g. dextran · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• G01N21/78

  producing a change of colour · CPC title

• G01N2021/7786

  Fluorescence · CPC title