Preparation of lactic acid and/or a lactate salt from lignocellulosic material by separate saccharification and fermentation steps

Patent metadata

Field	Value
Publication number	US-10240171-B2
Application number	US-201515329125-A
Country	US
Kind code	B2
Filing date	Jul 28, 2015
Priority date	Jul 28, 2014
Publication date	Mar 26, 2019
Grant date	Mar 26, 2019

Abstract

A process for preparing lactic acid and/or a lactate salt via the fermentation of carbohydrates obtained from lignocellulosic material. In particular the process includes: a) treating a lignocellulosic material with an alkaline agent including a caustic magnesium salt in the presence of water to provide a treated aqueous lignocellulosic material; b) saccharifying the treated aqueous lignocellulosic material in the presence of a hydrolytic enzyme to provide a saccharified aqueous lignocellulosic material including fermentable carbohydrates and a solid lignocellulosic fraction; c) fermenting the fermentable carbohydrates in the saccharified aqueous lignocellulosic material by way of lactic acid producing microorganism in the presence of an alkaline agent including a caustic magnesium salt to provide an aqueous fermentation broth including a magnesium lactate; and d) isolating lactic acid and/or lactate salt from the fermentation broth; wherein the saccharification step b) and the fermentation step c) are performed as two separate steps.

First claim

The invention claimed is:

1. A process for preparing lactic acid and/or a lactate salt comprising:
a) treating a lignocellulosic material with an alkaline agent comprising a caustic magnesium salt in the presence of water to provide a treated aqueous lignocellulosic material;
b) saccharifying the treated aqueous lignocellulosic material in the presence of a hydrolytic enzyme to provide a saccharified aqueous lignocellulosic material comprising fermentable carbohydrates and a solid lignocellulosic fraction;
c) fermenting the fermentable carbohydrates in the saccharified aqueous lignocellulosic material by means of a lactic acid producing microorganism in the presence of an alkaline agent comprising a caustic magnesium salt to provide an aqueous fermentation broth comprising a magnesium salt of lactic acid; and,
d) isolating lactic acid and/or a salt of lactic acid from the fermentation broth,
wherein the saccharification step b) and the fermentation step c) are performed as two separate steps.

2. The process according to claim 1 comprising subjecting the lignocellulosic material to at least one of acid treatment, solvent treatment and steam treatment prior to treating the lignocellulosic material with the alkaline agent of step a).

3. The process according to claim 1 comprising treating the lignocellulosic material with the alkaline agent of step a)
i) at a pH from 4.0 to 14.0;
ii) at a temperature from 15 to 250° C. and,
iii) for a time period of from 1 minute to 600 minutes.

4. The process according to claim 1 comprising subjecting the lignocellulosic material to acid treatment prior to treating said material with the alkaline agent of step a),
wherein said acid treatment comprises mixing said lignocellulosic material with an acidic aqueous solution having a concentration of 8 wt.% or less of acid selected from the group consisting of inorganic acid, organic acid, amino acid, mineral acid, Bronsted acid, Lewis acid and mixtures thereof, and
wherein said mixing takes place at a temperature of from 120° C. to 230° C.

5. The process according to claim 4 , wherein the treatment of step a) is performed
i) at a pH of from 2 to 10.
ii) at a temperature of from 15 to 100° C.
iii) for a period of from 1 to 30 minutes, to neutralize the acid treated lignocellulosic material.

6. The process according to claim 1 comprising performing the saccharification of step b) and/or the fermentation of step c)
i) at a pH from 2.0 to 10.0; and
ii) at a temperature from 30 to 80° C.

7. The process according to claim 1 comprising performing the saccharification of step b)
i) at a at a pH from 4.5 to 5.5; and
ii) at a temperature from 50 to 60° C.

8. The process according to claim 1 comprising adjusting the pH of the treated aqueous lignocellulosic material of step a) by acidification prior to the saccharification of step b), wherein acidification is performed with lactic acid.

9. The process according to claim 1 comprising subjecting the saccharified aqueous lignocellulosic material to a solid/liquid separation step prior to the fermentation of step c) to remove the solid lignocellulosic fraction from the saccharified lignocellulosic material comprising the fermentable carbohydrates.

10. The process according to claim 1 comprising subjecting the saccharified aqueous lignocellulosic material to a concentration step and/or a detoxification step prior to the fermentation of step c).

11. The process according to claim 1 comprising fermenting the saccharified aqueous lignocellulosic material
i) at a pH from 5.5 to 7.5, and
ii) at a temperature from 50 to 60° C.

12. The process of claim 1 , wherein in step d) magnesium lactate is isolated from the fermentation broth.

13. The process of claim 1 , wherein magnesium lactate is treated with an acid, selected from the group consisting of hydrochloric acid, sulphuric acid, and nitric acid.

14. The process of claim 13 , which comprises the steps of e) providing a feed comprising hydrogen chloride, said feed being either an aqueous solution comprising hydrogen chloride or a gas feed comprising gaseous hydrogen chloride; f) acidifying the recovered magnesium lactate to lactic acid by bringing said magnesium lactate into contact with said feed comprising hydrogen chloride, thereby forming a liquid effluent comprising lactic acid and magnesium chloride; g) separation of lignin from the liquid effluent product of step f); and, h) separation of the lactic acid and magnesium chloride present in the liquid effluent product of step g) to obtain a lactic acid product stream and a magnesium chloride solution or suspension.

15. The process of claim 1 , where the magnesium lactate is converted to a further lactate salt through a salt exchange process.

16. The process of claim 1 , wherein the magnesium lactate, further lactate salt, or lactic acid are subjected to one or more of liquid/liquid extraction, nanofiltration/active carbon treatment, distillation, and recrystallization.

17. The process of claim 1 , where prior to the fermentation step, the solid lignocellulosic fraction is removed.

Assignees

• Purac Biochem Bv

Inventors

• Baets Peter Johannes Marie
• Sanchez Garcia David
• Groot Willem Jacob
• De Haan André Banier

Classifications

• C12P7/56 Primary

  Lactic acid · CPC title

• D21C1/06

  with alkaline reacting compounds · CPC title

• C12P2201/00

  Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis · CPC title

• D21C11/0007

  Recovery of by-products, i.e. compounds other than those necessary for pulping, for multiple uses or not otherwise provided for (volatile compounds obtained by decomposition of spent liquors in order to regenerate them D21C11/125; compounds obtained by fractionating the liquors in order to regenerate them D21C11/0042) · CPC title

• D21C3/02

  with inorganic bases or alkaline reacting compounds, e.g. sulfate processes · CPC title