Active-ester-group-containing composition for producing fibers, and cell culture scaffold material using fibers produced from active-ester-group-containing composition

Patent metadata

Field	Value
Publication number	US-10240122-B2
Application number	US-201515118481-A
Country	US
Kind code	B2
Filing date	Feb 13, 2015
Priority date	Feb 14, 2014
Publication date	Mar 26, 2019
Grant date	Mar 26, 2019

Abstract

A composition for producing a fiber, containing (A) a polymer compound containing a unit structure represented by the formula (1) and a unit structure represented by the formula (2), (B) a crosslinking agent, (C) an acid compound, and (D) a solvent

wherein each symbol in the formulas (1) and (2) is as described in the DESCRIPTION.

First claim

The invention claimed is:

1. A composition for producing a fiber, comprising
(A) a polymer compound comprising a unit structure represented by the formula (1) and a unit structure represented by the formula (2),
(B) a crosslinking agent,
(C) an acid compound that promotes a crosslinking reaction of hydroxyl groups of polymer compound (A) with crosslinking agent (B), and
(D) a solvent

wherein
R 1 is a hydrogen atom or a methyl group,
Q 1 is an ester bond or amide bond,
R 2 is an alkyl group having 1-10 carbon atoms or an aromatic hydrocarbon group having 6-10 carbon atoms, wherein at least one hydrogen atom is substituted by a hydroxy group,

wherein
R 3 is a hydrogen atom or a methyl group, and
Q 2 is an active ester group.

2. The composition according to claim 1 , wherein the above-mentioned Q 2 is represented by the formula (5):

wherein Q 3 is an N-succinimide group, a p-nitrophenyl group or a pentafluorophenyl group.

3. The composition according to claim 1 , wherein the above-mentioned polymer compound has a weight average molecular weight of 1,000-1,000,000.

4. The composition according to claim 1 , wherein the above-mentioned solvent is a polar solvent.

5. A production method of a fiber, comprising a step of spinning the composition according to claim 1 to produce a spun fiber.

6. The method according to claim 5 , wherein the above-mentioned spinning is electrospinning.

7. The method according to claim 5 , comprising a step of heating the spun fiber at 70-300° C.

8. The method according to claim 5 , further comprising a step for immobilizing a cell adhesion substance on the fiber by reaction between the active ester group in the fiber and the cell adhesion substance.

9. A fiber produced by the method according to claim 5 .

10. A cell culture scaffold material comprising the fiber according to claim 9 .

Assignees

• Nissan Chemical Ind Ltd
• Univ Kyoto

Inventors

• Umezaki Makiko
• Kishioka Takahiro
• Nishino Taito
• Otani Ayako
• Kamei Kenichiro
• Liu Li
• Chen Yong

Classifications

• C08L33/14

  of esters containing halogen, nitrogen, sulfur, or oxygen atoms in addition to the carboxy oxygen · CPC title

• C12N2533/40

  Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers · CPC title

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C08L33/12

  Homopolymers or copolymers of methyl methacrylate · CPC title

• C08L2203/12

  used for fibers · CPC title