Wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan, preparation method and use thereof

Patent metadata

Field	Value
Publication number	US-10238768-B2
Application number	US-201715704673-A
Country	US
Kind code	B2
Filing date	Sep 14, 2017
Priority date	Oct 28, 2016
Publication date	Mar 26, 2019
Grant date	Mar 26, 2019

Abstract

A wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan, a preparation method and use thereof are provided. The sponge comprises squid ink polysaccharide and chitosan as carriers and calcium chloride as an initiator for blood coagulation, and the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan is achieved via a lyophilization technology. Particularly, the preparation method comprises slowly adding a squid ink polysaccharide solution to a chitosan solution to form a uniformly mixed solution, adding a calcium chloride solution to the uniformly mixed solution, forming a gel followed by the gel being frozen and then lyophilized, and achieving the product. The wound-healing and hemostatic sponge has a good absorbing-errhysis effect, a good pro-coagulant effect, fast hemostasis and a complete hemostatic effect, without secondary re-hemorrhage. Moreover, the wound-healing and hemostatic sponge can promote wound healing, re-epithelialization and repair of epidermis and dermis, with strong wound-healing ability, good biocompatibility, weak toxic side effects and irritation, belonging to a novel wound-healing and hemostatic material. Moreover, the preparation method is reliable, fast, and low cost.

First claim

What is claimed:

1. A preparation method for a wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan, comprising steps of:
preparing a squid ink polysaccharide and a chitosan as carriers and an initiator for blood coagulation, wherein the initiator for blood coagulation is a calcium chloride solution;
dissolving the squid ink polysaccharide in a first solvent to obtain a squid ink polysaccharide solution, and dissolving the chitosan in a second solvent to obtain a chitosan solution;
mixing and combining the squid ink polysaccharide solution, the chitosan solution, and the calcium chloride solution;
and applying a lyophilization technology to obtain the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan,
wherein the squid ink polysaccharide solution has a concentration of 0.4 to 0.8 mg/mL.

2. The preparation method according to claim 1 , wherein the steps comprising
slowly adding the squid ink polysaccharide solution to the chitosan solution, to form a uniformly mixed solution,
adding the calcium chloride solution to the uniformly mixed solution, to obtain a homogenous solution,
forming a gel from the homogenous solution, freezing the gel, and
applying the lyophilization technology to lyophilize the gel to obtain the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan.

3. The preparation method according to claim 1 , wherein the chitosan solution has a mass concentration of 1.5 to 3.5%, and the calcium chloride solution has a mass concentration of 2 to 6%.

4. The preparation method according to claim 1 , wherein a volume ratio of the squid ink polysaccharide solution, the chitosan solution and the calcium chloride solution is 4-6:13-17:1-3.

5. The preparation method according to claim 1 , wherein the squid ink polysaccharide solution has a concentration of 0.55 mg/mL, the chitosan solution has a mass concentration of 2.29%, the calcium chloride solution has a mass concentration of 2.82%, and a volume ratio of the squid ink polysaccharide solution, the chitosan solution and the calcium chloride solution is 5:15:2.

6. The preparation method according to claim 2 , wherein a condition for the step of freezing the gel is frozen at −30° C. to −10° C. for 10 to 15 hours.

7. A wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan achieved by the preparation method according to claim 1 .

8. A method for hemostasis and/or wound healing, comprising
applying the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan according to claim 7 onto a wound,
compressing the wound until hemostasis is achieved,
dressing the wound, and
replacing the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan regularly.

9. A wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan, obtained by the preparation method according to claim 2 .

10. A wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan, obtained by the preparation method according to claim 3 .

11. A wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan, obtained by the preparation method according to claim 4 .

12. A wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan, obtained by the preparation method according to claim 5 .

13. A wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan, obtained by the preparation method according to claim 6 .

14. A method for hemostasis and/or wound healing, comprising
applying the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan according to claim 9 onto a wound,
compressing the wound until hemostasis is achieved,
dressing the wound, and
replacing the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan regularly.

15. A method for hemostasis and/or wound healing, comprising
applying the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan according to claim 10 onto a wound,
compressing the wound until hemostasis is achieved,
dressing the wound, and
replacing the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan regularly.

16. A method for hemostasis and/or wound healing, comprising
applying the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan according to claim 11 onto a wound,
compressing the wound until hemostasis is achieved,
dressing the wound, and
replacing the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan regularly.

17. A method for hemostasis and/or wound healing, comprising
applying the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan according to claim 12 onto a wound,
compressing the wound until hemostasis is achieved,
dressing the wound, and
replacing the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan regularly.

18. A method for hemostasis and/or wound healing, comprising
applying the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan according to claim 13 onto a wound,
compressing the wound until hemostasis is achieved,
dressing the wound, and
replacing the wound-healing and hemostatic sponge of squid ink polysaccharide/chitosan regularly.

Assignees

• Univ Guangdong Ocean

Inventors

• Huang Na
• Li Sidong
• Hu Zhang
• Li Chengpeng
• Kong Songzhi
• Cheng Yu
• Hong Pengzhi
• Luo Rongqiong
• Ouyang Qianqian
• Li Gaorong

Classifications

• A61L24/0005

  Ingredients of undetermined constitution or reaction products thereof · CPC title

• A61L2400/04

  Materials for stopping bleeding · CPC title

• C08B37/003 Primary

  Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof · CPC title

• C08L5/08

  Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof · CPC title

• A61L24/02

  containing inorganic materials · CPC title