Bovine milk oligosaccharides

Patent metadata

Field	Value
Publication number	US-10238674-B2
Application number	US-201715655396-A
Country	US
Kind code	B2
Filing date	Jul 20, 2017
Priority date	Jul 12, 2010
Publication date	Mar 26, 2019
Grant date	Mar 26, 2019

Abstract

Oligosaccharides from bovine milk, whey and dairy products, and methods of producing bovine milk oligosaccharides are provided.

First claim

What is claimed is:

1. A composition comprising galacto-oligosaccharides (GOS) and/or fructooligosaccharides (FOS) and/or lactulose and/or lactitol and/or xylooligosaccharides and at least one oligosaccharide from bovine milk or milk product and an inoculum of Bifidobacterium longum subsp. infantis or B. breve , wherein the oligosaccharide is selected from the group consisting of:
an oligosaccharide consisting of 3 Hexose (Hex) moieties, 4 N acetyl hexosamine (HexNAc) moieties and 1 fucose (Fuc) moiety;
an oligosaccharide consisting of 4 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 3 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 5 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 4 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 3 Hex moieties, 6 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 3 Hex moieties and 6 HexNAc moieties,
an oligosaccharide consisting of 4 Hex moieties and 3 HexNAc moieties;
an oligosaccharide consisting of 3 Hex moieties and 4 HexNAc moieties;
an oligosaccharide consisting of 6 Hex moieties and 2 HexNAc moieties;
an oligosaccharide consisting of 4 Hex moieties and 4 HexNAc moieties;
an oligosaccharide consisting of 3 Hex moieties and 5 HexNAc moieties;
an oligosaccharide consisting of 5 Hex moieties and 4 HexNAc moieties;
an oligosaccharide consisting of 4 Hex moieties and 5 HexNAc moieties; and
a mixture thereof.

2. The composition of claim 1 comprising at least two oligosaccharides, wherein the at least two oligosaccharide are selected from the group consisting of:
an oligosaccharide consisting of 3 Hexose (Hex) moieties, 4 N acetyl hexosamine (HexNAc) moieties and 1 fucose (Fuc) moiety;
an oligosaccharide consisting of 4 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 3 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 5 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 4 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 3 Hex moieties, 6 HexNAc moieties, and 1 Fuc moiety;
an oligosaccharide consisting of 3 Hex moieties and 6 HexNAc moieties,
an oligosaccharide consisting of 4 Hex moieties and 3 HexNAc moieties;
an oligosaccharide consisting of 3 Hex moieties and 4 HexNAc moieties;
an oligosaccharide consisting of 6 Hex moieties and 2 HexNAc moieties;
an oligosaccharide consisting of 4 Hex moieties and 4 HexNAc moieties;
an oligosaccharide consisting of 3 Hex moieties and 5 HexNAc moieties;
an oligosaccharide consisting of 5 Hex moieties and 4 HexNAc moieties;
an oligosaccharide consisting of 4 Hex moieties and 5 HexNAc moieties.

3. The composition of claim 2 , further comprising a bovine milk protein, a soy protein, betalactoglobulin, whey, soybean oil or starch.

4. The composition of claim 2 , further comprising a non-milk lipid.

5. The composition of claim 1 , further comprising a bovine milk protein, a soy protein, betalactoglobulin, whey, soybean oil or starch.

6. The composition of claim 1 , further comprising a non-milk lipid.

7. The composition of claim 6 , wherein the composition is a liquid and the content of the at least one oligosaccharide in the composition is from 0.001-100 g/L.

8. The composition of claim 1 , wherein the composition is a solid.

9. A method of obtaining one or more of the oligosaccharides recited in claim 1 , comprising purifying the at least one oligosaccharide from bovine milk and further comprising mixing the oligosaccharide with GOS and/or FOS and/or lactulose and/or lactitol and/or xylooligosaccharides.

Assignees

• Univ California

Inventors

• German J Bruce
• Mills David
• Lebrilla Carlito B
• Barile Daniela
• Locascio Riccardo

Classifications

• A23L33/135

  Bacteria or derivatives thereof, e.g. probiotics · CPC title

• C08L5/00

  Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00 · CPC title

• C08B37/0003

  General processes for their isolation or fractionation, e.g. purification or extraction from biomass · CPC title

• A61K35/741

  Probiotics (probiotic yeast, e.g. saccharomyces A61K36/06) · CPC title

• A61K31/702 Primary

  Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages · CPC title