Systems and methods for sample preparation using sonication

Patent metadata

Field	Value
Publication number	US-10233479-B2
Application number	US-201615140902-A
Country	US
Kind code	B2
Filing date	Apr 28, 2016
Priority date	Mar 15, 2013
Publication date	Mar 19, 2019
Grant date	Mar 19, 2019

Abstract

Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens.

First claim

The invention claimed is:

1. A method of assaying a biological sample for the presence of pathogen-identifying material, comprising:
providing a device comprising a housing, and within said housing a sonicator having a sonicator tip; a vessel holder configured to hold a vessel having a vessel wall; a sample handling system configured to move said vessel within the housing, wherein the vessel is configured to hold said biological sample for sonication; a solenoid disposed to urge said sonicator tip towards the vessel wall when said solenoid is activated, wherein the sonicator tip is configured to contact the vessel wall effective to transfer ultrasonic energy from said sonicator to the vessel wall upon operation of the sonicator; a spring disposed so as to urge the sonicator tip away from the vessel wall; and a detector;
forming a mixture of the biological sample and at least one reagent that reacts with the pathogen-identifying material to generate a signal;
operating the sonicator to transfer the ultrasonic energy to the vessel holding the biological sample or the mixture; and
assaying for the presence of said pathogen-identifying material in said mixture by using the sample handling system to move the sonicated vessel to the detector and detecting the signal, wherein assaying for the presence of said pathogen-identifying material is selected from detecting the presence of said pathogen-identifying material in said biological sample, identifying said pathogen-identifying material in said biological sample, measuring an amount of said pathogen-identifying material in said biological sample, and combinations thereof.

2. The method of claim 1 , wherein said assaying comprises optical detection using said detector.

3. The method of claim 1 , wherein said biological sample comprises a sample selected from blood, urine, sputum, tears, material from a nasal swab, material from a throat swab, material from a cheek swab, and another bodily fluid, excretion, secretion, and tissue obtained from a subject.

4. The method of claim 1 , wherein said sonicator of said device comprises a sonicator horn comprising said sonicator tip.

5. The method of claim 1 , wherein said detector of said device comprises an optical detector.

6. The method of claim 1 , wherein said device further comprises a communication assembly configured to communicate with a user, a device, a laboratory, a network, or the cloud.

7. The method of claim 1 , wherein said device is configured to apply force from the sonicator to the vessel while applying said sonicator tip to a wall of said vessel held in said vessel holder.

8. A method of assaying a biological sample for the presence of pathogen-identifying material, comprising:
providing a system comprising a device configured to assay said sample for the presence of pathogen-identifying material, said device comprising a housing; a sonicator within said housing, said sonicator having a sonicator tip; a sample handling system configured to move a sample within the housing; a vessel holder configured to hold said vessel, the vessel having a vessel wall and being configured to hold said sample for sonication; a solenoid disposed to urge said sonicator tip towards said vessel wall when said solenoid is activated, wherein the sonicator tip is configured to contact the vessel wall effective to transfer ultrasonic energy from said sonicator to the vessel wall upon operation of the sonicator; a spring disposed so as to urge the sonicator away from the vessel wall; and a detector;
forming a mixture of the biological sample and at least one reagent that reacts with the pathogen-identifying material to generate a signal;
operating the sonicator to transfer the ultrasonic energy to the vessel holding the biological sample or the mixture; and
assaying for the presence of the pathogen-identifying material in said mixture by using the sample handling system to move the sonicated vessel to the detector and detecting the signal, wherein assaying for the presence of said pathogen-identifying material is selected from the group consisting of detecting the presence of the pathogen-identifying material in the biological sample, identifying the pathogen-identifying material in the biological sample, measuring an amount of the pathogen-identifying material in the biological sample, and combinations thereof.

9. The method of claim 8 , wherein said assaying comprises optical detection using said detector.

10. The method of claim 8 , wherein said biological sample comprises a sample selected from the group consisting of blood, urine, sputum, tears, material from a nasal swab, material from a throat swab, material from a cheek swab, and another bodily fluid, excretion, secretion, and tissue obtained from a subject.

11. The method of claim 8 , wherein said sonicator of said device comprises a sonicator horn comprising said sonicator tip.

12. The method of claim 8 , wherein said detector of said device comprises an optical detector.

13. The method of claim 8 , wherein said device further comprises a communication assembly configured to communicate with a user, a device, a laboratory, a network, or the cloud.

14. The method of claim 8 , wherein said device is configured to apply force from the sonicator to the vessel while applying said sonicator tip to a wall of said vessel held in said vessel holder.

Assignees

• Theranos Inc
• Theranos Ip Co Llc

Inventors

• Loo Alexander
• Holmes Elizabeth A

Classifications

• G01N2333/24

  from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia · CPC title

• G01N2333/31

  from Staphylococcus (G) · CPC title

• G01N1/38

  Diluting, dispersing or mixing samples · CPC title

• B01F11/0266

  Operations & Transport · mapped topic

• B01L2300/0609

  Holders integrated in container to position an object · CPC title