Culture medium for microorganisms including para-aminobenzoic acid as a selective agent

Patent metadata

Field	Value
Publication number	US-10233477-B2
Application number	US-201213978590-A
Country	US
Kind code	B2
Filing date	Jan 31, 2012
Priority date	Feb 1, 2011
Publication date	Mar 19, 2019
Grant date	Mar 19, 2019

Abstract

The field of the invention is the analysis of target microorganisms in a complex sample. The present invention more particularly relates to a culture medium for the detection of at least one target microorganism including: at least one natural or synthetic fermentation or enzymatic activity substrate, and at least one selective agent, which inhibits non-target microorganisms, constituted by para-amino benzoic acid, one of its derivatives or one of their salts, at a concentration of between 0.05 and 1 g/L.

First claim

The invention claimed is:

1. A culture medium for the detection of at least one target microorganism comprising:
at least one natural or synthetic fermentation or enzymatic substrate of the at least one target microorganism; and
selective agents, wherein the selective agents consist of:
bile salts,
at least one agent selected from the group consisting of para-amino benzoic acid, a derivative of para-amino benzoic acid, a salt thereof, and combinations thereof, and
optionally at least one of Novobiocin, Vancomycin, Amphotericin, and Cefsulodin;

wherein a concentration of the para-amino benzoic acid, a derivative of para-amino benzoic acid, and/or a salt thereof ranges from 0.05 to 1 g/L;
the derivative of para-amino benzoic acid comprises a para-amino benzoic skeleton;
the at least one target microorganism is a Salmonella genus bacterium;
the at least one natural or synthetic fermentation or enzymatic substrate comprises at least one chromogenic or fluorogenic substrate; and
a hydrolysation of the at least one natural or synthetic fermentation or enzymatic substrate distinguishes the at least one target microorganism from non-target microorganisms in the culture medium by causing a change in color or fluorescence, respectively, in the target microorganism.

2. The culture medium according to claim 1 , wherein the culture medium contains at least one component that promotes a culturing of the Salmonella genus bacterium.

3. The culture medium according to claim 1 , wherein the non-target microorganisms are selected from the group consisting of enterobacteria, except a Salmonella genus bacterium, and the culture medium contains at least one component that inhibits or slows a culturing of the non-target microorganisms.

4. The culture medium according to claim 1 , wherein the derivative of para-amino benzoic acid is selected from the group consisting of 4-(methylamino) benzoic acid, 4-amino-2-chlorobenzoic acid, 4-amino-3-nitro benzoic acid, and 4-aminosalicylic acid.

5. The culture medium according to claim 1 , wherein the bile salts comprise at least sodium deoxycholate.

6. The culture medium according to claim 1 , wherein the selective agents include the at least one of Novobiocin, Vancomycin, Amphotericin, and Cefsulodin.

7. The culture medium according to claim 1 , wherein the at least one natural or synthetic fermentation or enzymatic substrate is a metabolic substrate.

8. The culture medium according to claim 7 , wherein a degradation product of the metabolic substrate varies a pH of the culture medium.

9. The culture medium according to claim 1 , wherein the concentration of the para-amino benzoic acid, a derivative of para amino benzoic acid, and/or a salt thereof ranges from 0.3 to 1 g/L.

10. The culture medium according to claim 1 , wherein the concentration of the para-amino benzoic acid, a derivative of para-amino benzoic acid, and/or a salt thereof ranges from 0.5 to 1 g/L.

11. A culture medium for the detection of at least one target microorganism comprising:
at least three chromogenic substrates; and
selective agents, wherein the selective agents consist of:
bile salts,
at least one agent selected from the group consisting of para-amino benzoic acid, a derivative of para-amino benzoic acid, a salt thereof, and combinations thereof, and
optionally at least one of Novobiocin, Vancomycin, Amphotericin, and Cefsulodin;

wherein a concentration of the at least one agent ranges from 0.05 to 1 g/L;
the derivative of para-amino benzoic acid comprises a para-amino benzoic skeleton;
the at least one target microorganism is a Salmonella genus bacterium; and
a hydrolysation of one or more of the at least three chromogenic substrates distinguishes the at least one target microorganism from non-target microorganisms in the culture medium by causing a change in color in the target microorganism.

12. A process for detecting a target microorganism, in a mixed population of target and non-target microorganisms, comprising:
placing a sample, in which it is desired to detect the target microorganism in the sample, in contact with the culture medium according to claim 1 ;
incubating the culture medium at a temperature that promotes reproduction of the target microorganism; and
observing the microorganisms on the culture medium, wherein the target microorganism is a Salmonella genus bacterium.

13. A method comprising: detecting in vitro at least one target microorganism with the culture medium according to claim 1 , wherein the at least one target microorganism is a Salmonella genus bacterium.

14. A method comprising:
detecting at least one target microorganism with the culture medium of claim 1 .

Assignees

• Roche Jean Marc
• Biomerieux Sa

Inventors

• Roche Jean-Marc

Classifications

• C12Q1/02

  involving viable microorganisms · CPC title

• G01N2333/255

  Salmonella (G) · CPC title

• C12Q1/045 Primary

  Culture media therefor · CPC title

• C12Q1/10

  Enterobacteria · CPC title

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title