Methods of preconditioning pretreated cellulosic material

Patent metadata

Field	Value
Publication number	US-10233471-B2
Application number	US-201414891186-A
Country	US
Kind code	B2
Filing date	May 15, 2014
Priority date	May 16, 2013
Publication date	Mar 19, 2019
Grant date	Mar 19, 2019

Abstract

The invention relates to methods of preconditioning pretreated cellulosic material in the presence of a combination of phenol oxidizing enzyme and glucoamylase. The invention also relates to processes of producing sugars and fermentation products including a preconditioning step. Finally the invention relates to a composition suitable for preconditioning.

First claim

The invention claimed is:

1. A process of recovering oil from pretreated cellulosic material comprising the steps of:
preconditioning the pretreated cellulosic material with a phenol oxidizing enzyme and a glucoamylase;
(ii) saccharifying the preconditioned material with a cellulolytic enzyme preparation;
(iii) recovering oil from saccharified material in step (ii).

2. A process of producing a sugar from pretreated cellulosic material comprising the steps of:
preconditioning the pretreated cellulosic material with a phenol oxidizing enzyme and a glucoamylase;
(ii) saccharifying the preconditioned material with a cellulolytic enzyme preparation; and
(iii) recovering oil from saccharified material in step (ii).

3. The process of claim 2 , wherein the phenol oxidizing enzyme is a laccase.

4. The process of claim 2 , further comprising recovering sugars from the saccharified material from step (ii).

5. The process of claim 2 , wherein the glucoamylase is derived from a strain of Aspergillus.

6. The process of claim 2 , wherein the preconditioning further comprises addition of beta-glucosidase, alpha amylase, hemicellulase or combination thereof.

7. The process of claim 2 , wherein the pretreated cellulosic material is dilute acid pretreated or auto-hydrolyzed.

8. The process of claim 2 , wherein the pretreated cellulosic material is unwashed, un-detoxified or washed.

9. The process of claim 2 , wherein the sugar produced is increased as compared to when no preconditioning is done.

10. The process of claim 2 , wherein the oil is recovered and an increased amount of oil is released compared to when no preconditioning is done.

11. The process of claim 2 , wherein the glucoamylase is derived from a strain of Aspergillus niger or Aspergillus oryzae.

12. The process of claim 2 , wherein the glucoamylase comprises a polypeptide having the sequence of SEQ ID NO: 13.

Assignees

• Novozymes As
• Novozyme As

Inventors

• Li Xin
• Emme Brandon
• Putnam Lorraine
• Smith Mads Torry

Classifications

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12N9/0061

  Laccase (1.10.3.2) · CPC title

• C12Y302/01003

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12Y110/03002

  Laccase (1.10.3.2) · CPC title

• C12P7/16

  Butanols · CPC title