Device and method for wetting a sample with an aerosol

Patent metadata

Field	Value
Publication number	US-10233418-B2
Application number	US-201514861336-A
Country	US
Kind code	B2
Filing date	Sep 22, 2015
Priority date	Mar 22, 2013
Publication date	Mar 19, 2019
Grant date	Mar 19, 2019

Abstract

A device for wetting at least one sample by means of an aerosol, the device has: side walls, a base region which is bounded by the side walls, the base region adapted for receiving or interacting with a positioning device for positioning the sample, a cover region, which sits vertically opposite the base region and is bounded by the side walls. The device forms an exposure chamber and an aerosolizing device for producing an aerosol, which is arranged in the vicinity of the cover region. The aerosolizing device is arranged above the base region in such a way that the aerosolizing device at least partially covers the base region in a vertical plan view. A method for wetting at least one sample by an aerosol is also provided that includes the steps of: providing at least one sample, producing a cloud above the at least one sample in a position which at least partially covers the base region in a vertical plan view, and allowing the cloud to descend in the direction of the sample.

First claim

What is claimed is:

1. A device for wetting at least one sample by an aerosol, the device comprising:
side walls;
a base region bounded by the side walls; the base region being adapted for receiving or for interacting with a positioning device that positions the at least one sample;
a cover region that is vertically opposite the base region and delimited by the side walls; and
an aerosolizing device generating an aerosol is arranged in a vicinity of the cover region, the aerosolizing device being arranged above the base region such that the aerosolizing device at least partially covers the base region in a vertical plan view,
wherein the device forms an exposure chamber,
wherein the device is configured to operate without external air flow into or out of the device, and
wherein the aerosolizing device is arranged approximately 5 cm to 50 cm above the base region or the positioning device.

2. The device according to claim 1 , wherein the aerosolizing device has at least one nebulizer.

3. The device according to claim 1 , wherein the device and/or the aerosolizing device are designed to form an aerosol cloud.

4. The device according to claim 1 , wherein the device is configured to be airflow-free and to operate the nebulizer airflow-free.

5. The device according to claim 1 , wherein the aerosolizing device is oriented so that the initial direction of movement of the cloud forms an angle smaller than 30° with the vertical and is essentially parallel to a vertical.

6. The device according to claim 1 , wherein in the vertical plan view, the aerosolizing device is arranged approximately centrically above the base region or the positioning device.

7. The device according to claim 1 , wherein the device is configured such that a cloud initially generated by the aerosolizing device is such, that an average rate of fall of the cloud generated at the beginning of the exposure is greater than 1 cm/s, greater than 3 cm/s, or greater than 10 cm/s.

8. The device according to claim 1 , wherein the device is configured such that an aerosol cloud generated by the aerosolizing device is such that the aerosol cloud descends unobstructed and thus guarantees a rapid transport of the aerosol to the base region.

9. The device according to claim 1 , wherein the exposure chamber is dimensioned such that a horizontal cross-sectional area of the exposure chamber in the upper half of the chamber is more than 2-fold, 2.5-fold, or more than 3-fold as large as a cross-sectional area of an aerosol cloud initially generated.

10. The device according to claim 1 , further comprising a positioning device for positioning the at least one sample along the base region.

11. The device according to claim 1 , wherein the device and/or the positioning device is/are formed to receive or to wet a sample.

12. The device according to claim 1 , wherein the sample has biological material and/or material samples.

13. The device according to claim 1 , wherein the positioning device comprises elements for measuring and/or regulating temperature, elements for supplying the sample, or elements for measuring the weight of the substance deposited on one or more samples.

14. A method for wetting at least one sample by an aerosol with the device according to claim 1 , the method comprising:
providing the at least one sample in the exposure chamber;
generating an aerosol cloud above the at least one sample at a position that at least partially covers the base region in the vertical plan view; and
allowing the aerosol cloud to descend in a direction of the sample.

15. The method according to claim 14 , wherein as the aerosol cloud descends in the direction of the at least one sample, the aerosol cloud forms vortices that transform the cloud into a mist that uniformly fills the exposure chamber in a horizontal direction.

16. The method according to claim 15 , wherein the mist descends gravitationally in the direction of the sample.

17. The method according to claim 14 , further comprising the step of formation of vortices in the aerosol cloud by impingement of the aerosol cloud on the base region and/or the positioning device that provides the sample.

18. The method according to claim 14 , further comprising the step of forming a mist, wherein the mist descends gravitationally onto the at least one sample.

19. The method according to claim 14 , further comprising the step of providing an airflow-free aerosolizing device and/or an airflow-free nebulizer as the aerosolizing device to generate the aerosol.

20. The method according to claim 14 , further comprising a spatial uniform wetting of the at least one sample.

21. The device according to claim 13 , wherein the element for measuring and/or regulating temperature is a heater or an electrical temperature control device operated by a water circuit,
wherein the element for supplying the sample is a culture medium, and
wherein the elements for measuring the weight of the substance deposited on one or more samples are one or more quartz crystal microbalances.

22. The device according to claim 1 , wherein the aerosolizing device is arranged approximately 7 cm to 50 cm above the base region or the positioning device.

23. The device according to claim 1 , wherein the aerosolizing device is arranged approximately 9 cm to 20 cm above the base region or the positioning device.

Assignees

• Helmholtz Zentrum Muenchen Deutsches Forschungszentrum Gesundheit & Umwelt Gmbh
• Helmholtz Zentrum Muenchen Deutsche Forschungszentrum Fuer Gesundheit Und Umwelt Gmbh

Inventors

• Schmid Otmar
• Lenz Anke-G
• Lentner Bernd
• Eickelberg Oliver

Classifications

• G01N33/5014

  for testing toxicity · CPC title

• C12M25/06

  Plates; Walls; Drawers; Multilayer plates · CPC title

• C12M41/46

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• B05B16/00

  Spray booths (arrangements for collecting, re-using or eliminating excess spraying material in spray booths B05B14/40) · CPC title

• G01N33/5044

  involving specific cell types · CPC title