Aspiration tip

Patent metadata

Field	Value
Publication number	US-10232364-B2
Application number	US-201315038883-A
Country	US
Kind code	B2
Filing date	Nov 29, 2013
Priority date	Nov 29, 2013
Publication date	Mar 19, 2019
Grant date	Mar 19, 2019

Abstract

An aspiration tip is provided with: a syringe section including an inner tubular passage defining an aspiration path for aspirating an object; and a plunger movable in the tubular passage while coming in contact with an inner wall surface of the tubular passage. The syringe section includes an aspiration opening formed in a distal end of the tubular passage for aspirating the object, and the plunger includes a plunger leading end portion configured to protrude from the aspiration opening before the aspiration of the object and at the time of discharge of the object, and retract in the syringe section at the time of the aspiration of the object.

First claim

The invention claimed is:

1. An aspiration tip, comprising:
a syringe section including an inner tubular passage defining an aspiration path for aspirating an object; and
a plunger movable in the tubular passage while coming in contact with an inner wall surface of the tubular passage, wherein
the syringe section includes an aspiration opening formed in a distal end of the tubular passage for aspirating the object, and
the plunger includes a plunger leading end portion configured to protrude from the aspiration opening at the time of starting an aspiration of the object and at the time of finishing a discharge of the object, and retract in the syringe section at the time of the aspiration of the object, wherein
the syringe section includes a syringe base end portion and a syringe main body portion;
the plunger includes a plunger base end portion and a plunger main body portion;
the syringe base end portion includes a receiving part disposed on an inner wall surface of the syringe base end portion;
the plunger base end portion includes a contacting part disposed on an outer peripheral surface of the plunger base end portion; and
the contacting part comes to engage with the receiving part when the plunger leading end portion protrudes from the aspiration opening in the movement of the plunger in the tubular passage at the time of starting the aspiration of the object and at the time of finishing the discharge of the object; wherein
the syringe base end portion includes:
a syringe large diameter part in the form of a cylinder,
a syringe connecting part connecting the syringe large diameter part and the syringe main body portion, and
the receiving part disposed on an inner wall surface of the syringe connecting part;

the plunger base end portion includes:
a plunger large diameter part in the form of a cylinder and placed in the syringe large diameter part,
a plunger connecting part connecting the plunger large diameter part and the plunger main body portion, and

the contacting part defined by a plunger step part formed at a connecting position between the plunger large diameter part and the plunger connecting part; and
the receiving part is defined by a bulging part on the inner wall surface of the syringe connecting part that bulges toward an axial center of the syringe connecting part; and wherein
the plunger connecting part directly attached to the plunger large diameter part and directly attached to the plunger main body portion, the plunger step part being a first contact surface formed on the one end;
the bulging part includes a second contact surface facing to the first contact surface; and
at the time of starting the aspiration of the object and at the time of finishing the discharge of the object, the plunger is located at a position where the first contact surface of the plunger comes into contact with the second contact surface of the bulging part so that the plunger leading end portion protrudes from the aspiration opening by a predetermined length.

2. The aspiration tip according to claim 1 , wherein
the syringe section is made of a material having a higher rigidity than the plunger.

3. The aspiration tip according to claim 1 , wherein:
the syringe main body portion has a linear shape with one end connecting with the syringe base end portion and the other end opposite to the one end, the syringe main body portion having an outer periphery diametrically decreasing from the one end to the other end;
the plunger base end portion is placed in the syringe base end portion;
the plunger main body portion has one end connecting with the plunger base end portion and the other end opposite to the one end;
the tubular passage is disposed in the syringe main body portion and is in the form of a cylinder having a constant inner diameter; and
the plunger main body portion includes a first main body part in the form of a cylinder having a constant outer diameter, the first main body part being movable in the tubular passage of the plunger main body portion with a circumferential surface of the cylinder coming in contact with an inner wall surface of the syringe main body portion.

4. The aspiration tip according to claim 1 , wherein:
the syringe section has the other end opposite to the distal end bearing the aspiration opening; and
the plunger has the other end opposite to the plunger leading end portion, the aspiration tip further comprising:
a holder section attached to the other end of the syringe section and including an internal passage communicating with the tubular passage of the syringe section; and
a piston member attached to the other end of the plunger and movable in the holder section.

5. The aspiration tip according to claim 4 , wherein:
the syringe section is substantially oriented in a vertical direction; and
the holder section includes
a bent portion formed in an L-shape;
a first side of the bent portion being attached to the other end of the syringe section; and
a second side of the bent portion opposing to the first side extending in a horizontal direction.

6. The aspiration tip according to claim 1 , wherein
the object is a biological cell.

7. The aspiration tip according to claim 6 , wherein
the object is a biological cell aggregation.

8. The aspiration tip according to claim 1 , wherein:
the second conical part includes a peripheral part with a tapered surface, and
the tapered surface is tapered at the range of 30 to 80 degrees with respect to a leading end surface of the syringe section where the aspiration opening is formed.

Assignees

• Yamaha Motor Co Ltd

Inventors

• Ito Saburo

Classifications

• B01L3/022

  Capillary pipettes, i.e. having very small bore (B01L3/0224 - B01L3/0237 take precedence) · CPC title

• B01L2400/0478

  pistons · CPC title

• B01L3/0275 Primary

  Interchangeable or disposable dispensing tips · CPC title

• C12M33/04

  by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02) · CPC title

• B01L2200/0647

  Handling flowable solids, e.g. microscopic beads, cells, particles · CPC title