Fluid transport system and method for treating one or more tissue samples on a slide

Patent metadata

Field	Value
Publication number	US-10228382-B2
Application number	US-201314439808-A
Country	US
Kind code	B2
Filing date	Nov 1, 2013
Priority date	Nov 1, 2012
Publication date	Mar 12, 2019
Grant date	Mar 12, 2019

Abstract

A fluid transport system for an automated slide treatment apparatus having slide treatment modules is disclosed. The fluid transport system including a fluid dispensing robot configured by a controller to dispense a plurality of reagents to slides received in the slide treatment modules. The fluid dispensing robot includes pumping means configured by the controller to pump the reagents to be dispensed from a plurality of corresponding reagent containers; a probe having a body arranged to store one or more of the reagents pumped via the pumping means so as to prime the probe with said one or more of the reagents to be dispensed; and a well disposed on the body of the probe to store further of the reagents primed to be dispensed.

First claim

The invention claimed is:

1. A fluid transport system for treating one or more tissue samples disposed on slides, comprising:
an automated slide treatment apparatus including a plurality of slide treatment modules arranged to each receive one of the plurality of slides;
a fluid dispensing robot configured to dispense a plurality of reagents to each one of the slides received in the slide treatment modules to treat said one or more tissue samples respectively,
wherein the fluid dispensing robot includes:
a pumping means configured to pump the reagents to be dispensed from a plurality of corresponding reagent containers;
a probe having a body arranged to store one or more of the reagents pumped via the pumping means so as to prime the probe with said one or more of the reagents to be dispensed;
a well disposed on the body of the probe and arranged to store further of said one or more of the reagents so as to increase a volume of the one or more of the reagents primed to be dispensed; and

a driver means for the probe; wherein
the probe is arranged to dispense said one or more of the reagents primed to be dispensed to each one of the slides received in the slide treatment modules,
the fluid dispensing robot includes a nozzle disposed at an end of the probe and arranged to dispense said one or more of the reagents primed to be dispensed,
the slide includes a cover member with an inlet port,
the nozzle of the probe is arranged to couple with a chamfered opening of the cover member in said one of the slide treatment modules and substantially sealingly mate with the inlet port of said cover member while said one or more reagents are being dispensed,
the driver means continuously urges the nozzle of the probe towards the inlet port of said one of the slide treatment modules while the one or more reagents are being dispensed to maintain the seal with the inlet port, and
the probe and an interior of the slide treatment module are exposed to an ambient atmosphere prior to being sealingly mated.

2. A system as claimed in claim 1 , wherein the well and the body of the probe are removably attached to a reagent line connected to the pumping means at a resealable connector.

3. A system as claimed in claim 1 , wherein the body of the probe is removably attached to the fluid dispensing robot at one or more probe collars.

4. A system as claimed in claim 1 , wherein the fluid dispensing robot further includes a pressure sensor disposed between the well and the pumping means for sensing an amount of dispensed reagent from the probe.

5. A system as claimed in claim 1 , wherein the fluid transport system further includes a wash station for washing the probe when the probe is inserted in a wash drum thereof.

6. A system as claimed in claim 5 , wherein the wash drum includes a wash fluid injection port connected to a wash pump of the fluid transport system, wherein the wash pump is configured to pump wash fluid into the wash drum from one or more wash fluid containers.

7. A system as claimed in claim 6 , wherein the wash drum has a textured surface to create a turbulent flow of said wash fluid over the probe when inserted into the wash drum.

8. A system as claimed in claim 6 , wherein the wash drum further includes a collection chamber and scavenging port to collect and remove said wash fluid from the wash drum.

9. A system as claimed in claim 5 , wherein the wash drum includes an inlet port and an outlet port whereby a gas enters the wash drum through the inlet port to mix with the wash fluid around the probe and the gas exits through the outlet port.

10. A system as claimed in claim 9 , further including a vacuum means arranged to apply a vacuum force to the outlet port to draw gas into the inlet port and out of the outlet port.

11. A system as claimed in claim 1 , wherein the pumping means is further configured to pump and/or aspirate more than one of the reagents from different reagent containers successively with an air gap between successive reagents so as to prime the probe with a plurality of different reagents.

12. A system as claimed in claim 1 , wherein the driver means includes a stepper motor having a constant torque mode.

13. A method of transporting fluid for treatment of one or more tissue samples disposed on slides whereby each one of the slides are received in a plurality of slide treatment modules, the plurality of slide treatment modules each including a cover member having a chamfered opening, and a plurality of reagents are dispensed by at least one fluid dispensing robot to each one of the slides received in the slide treatment modules to treat said one or more tissue samples respectively, the method comprising:
pumping the reagents to be dispensed from a plurality of corresponding reagent containers;
storing one or more of the reagents pumped from the corresponding reagent containers in a body of a probe so as to prime the probe with said one or more of the reagents to be dispensed;
further storing said one or more of the reagents in a well disposed on the body of the probe so as to increase a volume of the one or more of the reagents primed to be dispensed; and
dispensing said one or more of the reagents primed to be dispensed to each one of the slides received in the slide treatment modules via a nozzle disposed at an end of the probe, whereby the nozzle of the probe is arranged to couple with the chamfered opening of the cover member in said one of the slide treatment modules and substantially sealingly mate with an inlet port of said cover member while said one or more reagents are being dispensed, wherein
the nozzle of the probe is continuously urged towards the inlet port of said one of the slide treatment modules while the one or more reagents are being dispensed to maintain the seal with the inlet port, and
the probe and an interior of the slide treatment module are exposed to an ambient atmosphere prior to being sealingly mated.

14. Computer program code which when executed by a controller implements the method of claim 13 .

15. A tangible computer readable medium comprising the program code of claim 14 .

Assignees

• Leica Biosystems Melbourne Pty

Inventors

• Dockrill Mark Brian
• Limon Martin
• Drummond Michael Houston
• Wilcock Mark
• Rodgers Brendyn

Classifications

• G01N35/1009

  Characterised by arrangements for controlling the aspiration or dispense of liquids · CPC title

• G01N35/0099

  comprising robots or similar manipulators (robots per se B25J) · CPC title

• G01N2035/00138

  Slides · CPC title

• G01N2035/1025

  Fluid level sensing · CPC title

• G01N35/1002

  Reagent dispensers · CPC title