Segmented multi-use automated assay cartridge

Patent metadata

Field	Value
Publication number	US-10228367-B2
Application number	US-201615366963-A
Country	US
Kind code	B2
Filing date	Dec 1, 2016
Priority date	Dec 1, 2015
Publication date	Mar 12, 2019
Grant date	Mar 12, 2019

Abstract

A segmented multi-use automated assay cartridge for conducting a fluid assay includes a plurality of microfluidic assay circuits having the fluid flow channels configured to perform an assay on a sample, each circuit is configured to receive fluids for performing the assay (e.g., sample, buffer, detection analyte, dye, etc.) and each circuit is also configured to dispense waste fluid of the assay to a corresponding fluidic waste channel. A waste tower is configured to received waste fluid from the fluidic waste channel and to dispense the waste fluid to a common waste reservoir, whereby the assay waste fluid from each circuit comingles in the common waste reservoir and does not comingle with waste fluid from other circuits in the device in the fluidic waste channel, which allows multiple different assays to be run by the cartridge at different times.

First claim

At least the following is claimed:

1. A microfluidic device for conducting a fluid assay, having at least one fluid flow channel configured to flow fluids to perform the assay, comprising:
a plurality of microfluidic assay circuits having the fluid flow channels configured to perform an assay on a sample, each circuit configured to receive sample fluid from a corresponding sample well, each circuit configured to receive a detection analyte associated with the assay being performed, each circuit configured to receive buffer fluid from a corresponding buffer bank, and each circuit configured to dispense waste fluid of the assay to a corresponding fluidic waste channel; and
at least one waste flow feature configured to receive the waste fluid from the corresponding fluidic waste channel, to dispense waste fluid to a common waste reservoir, and to prevent waste fluid in the common waste reservoir from flowing into the fluidic waste channels; and
whereby the waste fluid from the fluidic waste channels comingles in the common waste reservoir and waste fluid in the common waste reservoir does not comingle with waste fluid in the fluidic waste channels.

2. The device of claim 1 , wherein a predetermined number of the plurality of assay circuits share a common waste channel and a common buffer bank.

3. The device of claim 1 , wherein a first set of the plurality of assay circuits share a first common waste channel and a first common buffer bank, and a second set, different from the first set, of the plurality of assay circuits share a second common waste channel and a second common buffer bank.

4. The device of claim 1 , wherein at least one circuit has at least one element disposed in the fluid flow channel, the element being positioned for exposure to fluid flows within the circuit for conducting the assay.

5. The device of claim 4 , wherein the element comprises a tubular element having a length less than 500 microns.

6. The device of claim 1 , further comprising a removable strip covering a predetermined number of the sample wells.

7. A microfluidic device for conducting a fluid assay, having at least one fluid flow channel configured to flow fluids to perform the assay, comprising:
a first set of microfluidic assay circuits each having the fluid flow channels configured to perform an assay on a first sample, each circuit in the first set configured to receive the first sample from a corresponding first sample well, each circuit in the first set configured to receive a first detection analyte associated with the assay being performed by that circuit, each circuit in the first set configured to receive buffer fluid from a common first buffer bank, and each circuit in the first set configured to dispense waste fluid of the assay to a common first fluidic waste channel;
a first waste flow feature fluidically connected to the first fluidic waste channel, the first waste flow feature configured to receive waste fluid from the first fluidic waste channel, to dispense waste fluid to a common waste reservoir, and to prevent waste fluid in the common waste reservoir from flowing into the first fluidic waste channel;
a second set of microfluidic assay circuits each having the fluid flow channels configured to perform an assay on a second sample, each circuit in the second set configured to receive the second sample from a corresponding second sample well, each circuit in the second set configured to receive a second detection analyte associated with the assay being performed by that circuit, each circuit in the second set configured to receive the buffer fluid from a common second buffer bank, and each circuit in the second set configured to dispense waste fluid of the assay to a common second fluidic waste channel;
a second waste flow feature fluidically connected to the second fluidic waste channel, the second waste flow feature configured to receive waste fluid from the common second fluidic waste channel, to dispense waste fluid to the common waste reservoir, and to prevent waste fluid in the common waste reservoir from flowing into the second fluidic waste channels; and
whereby the waste fluid from the first and second fluidic waste channels comingles in the common waste reservoir and waste fluid in the common waste reservoir does not comingle with waste fluid in the first and second fluidic waste channels.

8. The device of claim 7 , wherein at least one circuit has at least one element disposed in the fluid flow channel, the element being positioned for exposure to fluid flows within the circuit for conducting the assay.

9. The device of claim 8 , wherein the element comprises a tubular element having a length less than 500 microns.

10. The device of claim 7 , further comprising a first removable strip covering the sample wells corresponding to the first set of circuits and a second removable strip covering the sample wells corresponding to the second set of circuits.

11. The device of claim 7 wherein the assay is a protein-based assay.

12. The device of claim 1 , wherein the waste flow feature is configured to prevent waste fluid below a predetermined level in the common waste reservoir from entering the flow feature.

13. The device of claim 1 , wherein the waste flow feature comprises a waste tower.

14. The device of claim 1 , wherein each circuit is configured to receive a dye which binds to the detection analyte to optically measure assay results.

15. The device of claim 1 , wherein each circuit has at least one pneumatic actuated valve that controls fluid flow in the circuit and wherein a wall of one of the fluid flow channels is used as one side of a valve seat for the valve.

16. The device of claim 15 , wherein the wall is used as one side of a valve seat for at least one of the valves in a plurality of the assay circuits.

17. The device of claim 1 , wherein the fluid flow channels are made from injection-molded silicone.

18. The device of claim 7 , wherein at least one of the first and second waste flow features comprises a waste tower having a tower height and configured to prevent waste fluid below the tower height in the common waste reservoir from entering the tower.

19. The device of claim 7 , wherein each set of assay circuits comprises an “assay strip” configured to perform an assay independent of other assay strips in the device.

20. A microfluidic device for conducting a fluid assay, having at least one fluid flow channel configured to flow fluids to perform the assay, comprising:
a plurality of microfluidic assay circuits having the fluid flow channels configured to perform an assay on a sample, each circuit configured to receive sample fluid from a corresponding sample well, each circuit configured to receive a detection analyte associated with the assay being performed, each circuit configured to receive buffer fluid from a corresponding buffer bank, and each circuit configured to dispense waste fluid of the assay to a corresponding fluidic waste channel; and
at least one waste flow feature configured to receive the waste fluid from the corresponding fluidic waste channel, to dispense waste fluid to a common waste reservoir, and to prevent waste fluid in the common waste reservoir from flowing into the fluidic waste channels;
whereby the waste fluid from the fluidic waste channels comingles in the common waste reservoir and waste fluid in the common waste reservoir does not comingle with waste fluid in the fluidic waste channels; and
wherein a predetermined number of the assay circuits comprises an “assay str

Assignees

• ProteinSimple

Inventors

• Stanwood Charles
• Daigneault Noel
• Branciforte Jeffrey

Classifications

• B01L2300/021

  Identification, e.g. bar codes · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• B01L2300/0867

  Multiple inlets and one sample wells, e.g. mixing, dilution · CPC title

• B01L2300/0887

  Laminated structure · CPC title