Processes for producing fermentation products

Patent metadata

Field	Value
Publication number	US-10227613-B2
Application number	US-201314648477-A
Country	US
Kind code	B2
Filing date	Nov 26, 2013
Priority date	Mar 30, 2012
Publication date	Mar 12, 2019
Grant date	Mar 12, 2019

Abstract

The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention.

First claim

The invention claimed is:

1. A process for producing fermentation products from starch-containing material comprising the steps of:
i) liquefying the starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase in the presence of a protease;
ii) saccharifying the liquefied starch-containing material using a carbohydrate-source generating enzyme; and
iii) fermenting using a fermenting organism;
wherein a cellulolytic composition comprising two or more enzymes selected from the group consisting of an endoglucanase, a beta-glucosidase, a cellobiohydrolase, and a polypeptide having cellulolytic enhancing activity is present or added during fermentation or simultaneous saccharification and fermentation.

2. The process of claim 1 , wherein the pH during liquefaction is in the range of 5.0-6.5.

3. The process of claim 1 , wherein the fermentation product is ethanol.

4. The process of claim 1 , wherein the alpha-amylase is from the genus Bacillus.

5. The process of claim 4 , wherein the Bacillus stearothermophilus alpha-amylase has a double deletion of positions I181+G182 and optionally a N193F substitution, or deletion of R179+G180 (using SEQ ID NO: 1 for numbering).

6. The process of claim 1 , further wherein a glucoamylase is present and/or added during saccharification and/or fermentation.

7. The process of claim 6 , wherein the glucoamylase present and/or added during saccharification and/or fermentation is of fungal origin.

8. A process for producing fermentation products from starch-containing material, comprising the steps of:
i) liquefying the starch-containing material at a temperature above the initial gelatinization temperature using:
an alpha-amylase derived from Bacillus stearothermophilus;
a protease having a thermostability value of more than 20% determined as Relative Activity at 80° C./70° C., wherein the protease is derived from Pyrococcus furiosus or Thermoascus aurantiacus ; and
a Penicillium oxalicum glucoamylase;

ii) saccharifying the liquefied starch-containing material using a glucoamylase enzyme; and
iii) fermenting using a fermenting organism;
wherein a cellulolytic composition comprising two or more enzymes selected from the group consisting of an endoglucanse, a beta-glucosidase, a cellobiohydrolase, and a polypeptide having cellulolytic enhancing activity is present or added during fermentation or simultaneous saccharification and fermentation.

9. The process of claim 1 , wherein the cellulolytic composition is derived from a strain of Trichoderma reesei, Humicola insolens , or Chrysosporium lucknowense.

10. The process of claim 1 , wherein the cellulolytic composition is a Trichoderma reesei cellulolytic enzyme composition further comprising Penicillium emersonii GH61A polypeptide having cellulolytic enhancing activity disclosed in SEQ ID NO: 23 herein and Aspergillus fumigatus beta-glucosidase disclosed in SEQ ID NO: 22 herein or a variant thereof with the following substitutions: F100D, S283G, N456E, F512Y.

11. The process of claim 1 , wherein the pH during liquefaction is in the range of 5.0-6.0.

12. The process of claim 1 , wherein the pH during liquefaction is around 5.2.

13. The process of claim 1 , wherein the pH during liquefaction is around 5.4.

14. The process of claim 1 , wherein the pH during liquefaction is around 5.6.

15. The process of claim 1 , wherein the pH during liquefaction is around 5.8.

16. The process of claim 1 , wherein the alpha-amylase is a Bacillus stearothermophilus alpha-amylase having the amino acid sequence of SEQ ID NO: 1 or a variant thereof having at least 90% amino acid sequence identity thereto.

17. The process of claim 16 , wherein the alpha-amylase has at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 1.

18. The process of claim 16 , wherein the alpha-amylase has at least 97% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 1.

19. The process of claim 1 , wherein the protease is a variant of the metallo protease derived from a strain of Thermoascus aurantiacus.

20. The process of claim 1 , wherein the protease is derived from a strain of Pyrococcus furiosus.

21. The process of claim 1 , wherein a carbohydrate source generating enzyme is present or added during liquefaction step (i).

22. The process of claim 21 , wherein the carbohydrate source generating enzyme is a glucoamylase.

23. The process of claim 21 , wherein the carbohydrate source generating enzyme is a Penicillium oxalicum glucoamylase.

24. The process of claim 1 , wherein the cellulolytic composition is a Trichoderma reesei cellulolytic composition comprising three or more enzymes selected from the group consisting of an endoglucanase, a beta-glucosidase, a cellobiohydrolase, and a polypeptide having cellulolytic enhancing activity.

25. The process of claim 1 , wherein the cellulolytic composition is a Trichoderma reesei cellulolytic composition comprising four or more enzymes selected from the group consisting of an endoglucanase, a beta-glucosidase, a cellobiohydrolase, and a polypeptide having cellulolytic enhancing activity.

26. The process of claim 1 , wherein the starch-containing material is corn.

27. The process of claim 1 , wherein the cellulolytic composition is derived from a strain of Trichoderma reesei.

28. The process of claim 8 , wherein the starch-containing material is corn.

29. The process of claim 8 , wherein the cellulolytic composition is derived from a strain of Trichoderma reesei.

30. The process of claim 8 , wherein the fermentation product is ethanol.

Assignees

• Novozymes As

Inventors

• Deinhammer Randall
• Craig Joyce
• Matsui Tomoko
• Takagi Shinobu
• Clark Suzanne
• Matthews John
• Hjulmand Anne Glud
• Soong Chee-Leong
• Kang Zhengfang

Classifications

• C12N9/2414 Primary

  Alpha-amylase (3.2.1.1.) · CPC title

• C12N9/52

  derived from bacteria {or Archaea} · CPC title

• C13K1/06

  obtained by saccharification of starch or raw materials containing starch · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12P7/06 Primary

  Ethanol, i.e. non-beverage · CPC title