Methods and nucleic acid molecules for aav vector selection
US-2024417717-A1 · Dec 19, 2024 · US
Modified parvovirus useful for gene silencing
US10227609B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10227609-B2 |
| Application number | US-201314355691-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 25, 2013 |
| Priority date | Jan 27, 2012 |
| Publication date | Mar 12, 2019 |
| Grant date | Mar 12, 2019 |
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Abstract
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Described are methods for efficiently down regulating the expression of a gene of interest in a cell by use of a modified rodent parvovirus that contains an expressible target specific nucleic acid, preferably an shRNA expression cassette. Also described are cells or organisms comprising said parvovirus.
First claim
Opening claim text (preview).
The invention claimed is: 1. A modified parvovirus having a modified H-1 parvoviral genome comprising a non-coding region downstream of a parvovirus VP gene, wherein the modified H-1 parvoviral genome comprises the nucleotide sequence of SEQ ID NO:15 comprising the following mutations: (i) a deletion of nucleotides 2022-2135 of SEQ ID NO:15; and (ii) an insertion within the non-coding region downstream of the parvovirus VP gene, wherein the insertion is a nucleic acid molecule encoding a short hairpin RNA (shRNA) transcript under the control of a RNA polymerase III H1 promoter, wherein the shRNA is specific to a target gene, and wherein the modified parvovirus is capable of replicating and propagating in a cell autonomously. 2. The modified parvovirus of claim 1 , wherein the target gene is a disease-causing gene. 3. The modified parvovirus of claim 2 , wherein the disease-causing gene is a pathogenic animal virus gene, a cancer-related gene, an oncogene, anti-apoptotic gene, a gene critical for tumour cell growth, metastasis, angiogenesis or chemioresistance, an immunomodulatory gene, or a gene encoding a cytokine, growth factor, enzyme or transcription factor. 4. A composition comprising the modified parvovirus of claim 1 , further comprising a solvent suitable for intravenous (i.v.), intratumoral or endobronchial administration. 5. A cell containing the modified parvovirus of claim 1 .
Assignees
Inventors
Classifications
viral genome or elements thereof as genetic vector · CPC title
Specially adapted vectors · CPC title
Special delivery means, e.g. tissue-specific · CPC title
Stem-loop; Hairpin · CPC title
- C12N15/86Primary
Viral vectors · CPC title
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- What does patent US10227609B2 cover?
- Described are methods for efficiently down regulating the expression of a gene of interest in a cell by use of a modified rodent parvovirus that contains an expressible target specific nucleic acid, preferably an shRNA expression cassette. Also described are cells or organisms comprising said parvovirus.
- Who is the assignee on this patent?
- Deutsches Krebsforsch
- What technology area does this patent fall under?
- Primary CPC classification C12N15/86. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 12 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).