Method of increasing photosynthesis and yield of plants

Patent metadata

Field	Value
Publication number	US-10227606-B2
Application number	US-201414775110-A
Country	US
Kind code	B2
Filing date	Mar 11, 2014
Priority date	Mar 12, 2013
Publication date	Mar 12, 2019
Grant date	Mar 12, 2019

Abstract

The present invention pertains to a method for increasing the photosynthesis and yield/growth of plants, and a transgenic plant which is used in the method.

First claim

The invention claimed is:

1. A transgenic plant that overexpresses a cell membrane H + -ATPase (AHA2) encoding gene under control of a promoter strongly expressed specifically in guard cells wherein the AHA2 encoding gene encodes a cell membrane H + -ATPase comprising the full length amino acid sequence encoded by the nucleotide sequence of SEQ ID NO:1 and wherein the promoter comprises a nucleotide sequence having a sequence identity of 95% or more to the nucleotide sequence of SEQ ID NO:2.

2. The transgenic plant of claim 1 , which is a dicotyledon.

3. The transgenic plant of claim 2 , which belongs to the Brassicaceae family.

4. The transgenic plant of claim 3 , which belongs to the Arabidopsis genus or the Brassica genus.

5. The transgenic plant of claim 3 , which is Arabidopsis thaliana, Brassica napus, Brassica rapa var. nippo - oleifera, Brassica oleracea var. capitata, Brassica oleracea var. italica, Brassica oleracea var. botytis or Brassica rapa var. pekinensis.

6. The transgenic plant of claim 1 , which is a monocotyledon.

7. The transgenic plant of claim 6 , which belongs to the Poaceae family.

8. The transgenic plant of claim 7 , which belongs to the Oryza genus, the Zea genus, the Saccharum genus or the Sorghum genus.

9. The transgenic plant of claim 7 , in which the plant is Sorghum bicolor, Oryza sativa, Oryza glaberrima, Saccharum officinarum, Zea mays, Hordeum vulgare or Triticum aestivum.

10. A method for increasing yield of a plant or for increasing photosynthesis or for increasing growth in a plant comprising introducing an AHA2 encoding gene under control of a promoter strongly expressed specifically in guard cells into the plant, wherein the AHA2 encoding gene encodes a cell membrane H + -ATPase comprising the full length amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1 and wherein the promoter comprises a nucleotide sequence having a sequence identity of 95% or more to the nucleotide sequence of SEQ ID NO: 2.

11. The method of claim 10 , in which the plant is a dicotyledon.

12. The method of claim 11 , in which the plant belongs to the Brassicaceae family.

13. The method of claim 12 , in which the plant belongs to the Arabidopsis genus or the Brassica genus.

14. The method of claim 12 , in which the plant is Arabidopsis thaliana, Brassica napus, Brassica rapa var. nippo - oleifera, Brassica oleracea var. capitata, Brassica oleracea var. italica, Brassica oleracea var. botrytis or Brassica rapa var. pekinensis.

15. The method of claim 10 , in which the plant is a monocotyledon.

16. The method of claim 15 , in which the plant belongs to the Poaceae family.

17. The method of claim 16 , in which the plant belongs to the Oryza genus, the Zea genus, the Saccharum genus or the Sorghum genus.

18. The method of claim 16 , in which the plant is Sorghum bicolor, Oryza sativa, Oryza glaberrima, Saccharum officinarum, Zea mays, Hordeum vulgare or Triticum aestivum.

19. The transgenic plant of claim 1 , wherein said promoter has a sequence identity of 99% or more to the nucleotide sequence of SEQ ID NO: 2.

20. The method of claim 10 , wherein said promoter has a sequence identity of 99% or more to the nucleotide sequence of SEQ ID NO: 2.

21. The transgenic plant of claim 1 , wherein said promoter comprises the nucleotide sequence of SEQ ID NO: 2.

22. The method of claim 10 , wherein said promoter comprises the nucleotide sequence of SEQ ID NO: 2.

23. The transgenic plant of claim 1 , wherein said AHA2 encoding gene comprises the nucleotide sequence of SEQ ID NO: 1.

24. The method of claim 10 , wherein said AHA2 encoding gene comprises the nucleotide sequence of SEQ ID NO: 1.

25. The transgenic plant of claim 23 , wherein said promoter comprises the nucleotide sequence of SEQ ID NO: 2.

26. The method of claim 24 , wherein said promoter comprises the nucleotide sequence of SEQ ID NO: 2.

Assignees

• Univ Nagoya Nat Univ Corp

Inventors

• Kinoshita Toshinori
• Wang Yin
• Inoue Shin-Ichiro
• Ono Natsuko

Classifications

• C12N9/00

  Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00) · CPC title

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8225

  Leaf-specific, e.g. including petioles, stomata · CPC title