Combined thermal devices for thermal cycling

Patent metadata

Field	Value
Publication number	US-10226772-B2
Application number	US-201615056475-A
Country	US
Kind code	B2
Filing date	Feb 29, 2016
Priority date	Jun 30, 2006
Publication date	Mar 12, 2019
Grant date	Mar 12, 2019

Abstract

The invention relates to systems and methods including a combination of thermal generating device technologies to achieve more efficiency and accuracy in PCR temperature cycling of nucleic samples undergoing amplification.

First claim

What is claimed is:

1. A nucleic acid amplification system comprising:
a microfluidic device comprising at least one microfluidic channel,
means to flow a fluid sample through the at least one microfluidic channel,
a first heating device to heat and cool the fluid sample to achieve nucleic acid amplification,
a second heating device to heat and cool the fluid sample to achieve nucleic acid amplification,
a first temperature sensing device to sense the temperature of the fluid sample,
a second temperature sensing device to sense the temperature of the fluid sample, and
a controller to energize and deenergize the first and second heating devices based on readings provided by the first and second temperature sensing device, wherein the first and second heating devices have different temperature resolutions.

2. The system of claim 1 , wherein the fluid flow means causes a pressure differential.

3. The system of claim 2 , wherein the fluid flow means is a pump.

4. The system of claim 1 , wherein the first heat source is a contact heat source.

5. The system of claim 4 , wherein the first heat source is selected from the group comprising: a Peltier device, a resistive heater or a chemical heater.

6. The system of claim 1 , wherein the second heat source is a non-contact heat source.

7. The system of claim 6 , wherein the second heat source is a electromagnetic heat source.

8. The system of claim 7 , wherein the second heat source is selected from the group comprising an infra-red source, a tungsten filament bulb and laser.

9. The system of claim 1 , wherein the controller is configured to independently adjust the first and second heating device.

10. The system of claim 9 , wherein the controller is configured to increase heat from the second heating device when decreasing heat from the first heating device.

11. The system of claim 1 , wherein the first heating device is thermostatically controlled by the controller based on an output of the first temperature sensing device, which temperature sensing device is operable to sense a temperature of the sample.

Assignees

• Canon Us Life Sciences Inc

Inventors

• Dale Gregory A
• Zeng Shulin
• Hasson Kenton C

Classifications

• B01L7/52

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• B01L2300/0654

  Lenses; Optical fibres · CPC title

• Y10T436/117497

  with a continuously flowing sample or carrier stream · CPC title