Methods and devices for sequencing nucleic acids in smaller batches

Patent metadata

Field	Value
Publication number	US-10222349-B2
Application number	US-201715656307-A
Country	US
Kind code	B2
Filing date	Jul 21, 2017
Priority date	Feb 5, 2007
Publication date	Mar 5, 2019
Grant date	Mar 5, 2019

Abstract

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.

First claim

The invention claimed is:

1. An automated nucleic acid sequencing system, comprising:
a) flow cells comprising nucleic acid to be sequenced, said flow cells mounted on
b) a moveable support controlled by a
c) software user interface, said support configured to move flow cells to
c) reagent delivery stations, each reagent delivery station in fluidic communication with
d) reagent reservoirs configured to supply extension reagents including fluorescent nucleotide analogues,
e) an imaging platform configured to detect said fluorescent nucleotide analogues, and
f) a transfer means configured for moving at least one of said flow cells from said moveable support to said imaging platform.

2. The system of claim 1 , wherein said imaging platform is associated with a camera, said camera positioned for imaging one or more flow cells.

3. The system of claim 2 , wherein said imaging platform is configured for x/y movement and said imaging platform is positioned under said camera.

4. The system of claim 1 , wherein said system is contained within the housing of an instrument, said software user interface mounted on said housing, said software user interface in communication with a programmable processor.

5. The system of claim 1 , wherein said moveable support is a carousel.

6. The system of claim 1 , further comprising an actuator configured for raising said moveable support so as to disconnect said flow cells from said stations.

Assignees

• Intelligent Biosystems Inc
• Qiagen Sciences Llc

Inventors

• Gordon Steven
• Hagerott Thomas
• Golaski Edmund
• Olejnik Jerzy

Classifications

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L2300/0877

  Flow chambers · CPC title

• G01N27/44791 Primary

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title