Method and device for optically detecting nanoparticles in a fluid sample

Patent metadata

Field	Value
Publication number	US-10222319-B2
Application number	US-201515517723-A
Country	US
Kind code	B2
Filing date	Sep 29, 2015
Priority date	Oct 9, 2014
Publication date	Mar 5, 2019
Grant date	Mar 5, 2019

Abstract

A device for optically detecting in transmission nanoparticles moving in a fluid sample includes a light source for emitting a spatially incoherent beam for illuminating the sample; an imaging optical system; and a two-dimensional optical detector. The imaging optical system includes a microscope objective. The two-dimensional optical detector includes a detection plane conjugated with an object focal plane of the microscope objective by said imaging optical system. The two-dimensional optical detector allows a sequence of images of an analysis volume of the sample to be acquired, each image resulting from optical interferences between the illuminating beam incident on the sample and the beams scattered by each of the nanoparticles present in the analysis volume during a preset duration shorter than one millisecond. The device further includes an image processor that allows an average of a sequence of said images to be taken and said average to be subtracted from each image in order to determine, for each nanoparticle of the analysis volume, the amplitude of the scattered beam.

First claim

The invention claimed is:

1. A device for optically detecting in transmission nanoparticles moving in a fluid sample, comprising:
a light source for emitting a spatially incoherent beam for illuminating the sample;
an imaging optical system comprising a microscope objective that collects beams scattered by each of the nanoparticles and the incoherent beam passed through the sample in transmission;
a two-dimensional optical detector comprising a detection plane conjugated with an object focal plane of the microscope objective by said imaging optical system, and allowing a sequence of images of an analysis volume of the sample to be acquired, each image resulting from optical interferences between the incoherent beam passed through the sample and the beams scattered by each of the nanoparticles present in the analysis volume during a preset duration shorter than one millisecond; and
a processor that averages a sequence of said images to be taken and said average to be subtracted from each image in order to determine, for each nanoparticle of the analysis volume, the amplitude of the scattered beam.

2. The device according to claim 1 , wherein the light source is a pulsed source, enabling sequential emitting of light pulses of said preset duration, and the processor synchronizes the two-dimensional optical detector and the pulsed light source for the acquisition of said sequence of images.

3. The device according to claim 1 , wherein the microscope objective used has a numerical aperture greater than or equal to 1.

4. A method for optically detecting in transmission nanoparticles moving in a fluid sample, comprising:
emitting a spatially incoherent light beam for illuminating the sample;
using an imaging optical system comprising a microscope objective, that collects beams scattered by each of the nanoparticles and the incoherent beam passed through the sample in transmission, to form, on a detection plane of a two-dimensional optical detector, images of an analysis volume of the sample located in the vicinity of an object focal plane of the microscope objective;
acquiring, from the two-dimensional detector, a sequence of images of the analysis volume of the sample, each image resulting from optical interferences between the incoherent beam passed through the sample and the beams scattered by each of the nanoparticles present in the analysis volume during a preset duration shorter than one millisecond; and
processing the images to take an average of a sequence of said images and subtract said average from each image in order to determine, for each nanoparticle of the analysis volume, the amplitude of the scattered beam.

5. The method according to claim 4 , wherein:
emitting the light beam comprises sequentially emitting light pulses of said preset duration; and
acquiring the images is synchronized with the emission of the light pulses.

6. The method according to claim 4 , further comprising:
determining trajectories of the nanoparticles starting from the sequence of processed images.

7. The device according to claim 2 , wherein the microscope objective used has a numerical aperture greater than or equal to 1.

8. The method according to claim 5 , further comprising:
determining trajectories of the nanoparticles starting from the sequence of processed images.

Assignees

• Ecole Superieure De Physique Et De Chimie Ind De La Ville De Paris—Espci Paristech
• Ecole Superieure Physique & Chimie Ind Ville De Paris
• Centre Nat Rech Scient
• Univ De Pierre Et Marie Curie
• Ecole Normale Superieure
• Inst Nat Sante Rech Med

Inventors

• Boccara Albert Claude
• Boccara Martine

Classifications

• G02B21/0056

  based on optical coherence, e.g. phase-contrast arrangements, interference arrangements · CPC title

• G01N2015/0038

  Investigating nanoparticles · CPC title

• G02B21/14

  affording illumination for phase-contrast observation · CPC title

• G06T2207/10056

  Microscopic image · CPC title

• G01N15/1434

  Optical arrangements · CPC title