Modular instrumentation for analyzing biological fluids

Patent metadata

Field	Value
Publication number	US-10220387-B2
Application number	US-201415101061-A
Country	US
Kind code	B2
Filing date	Dec 5, 2014
Priority date	Dec 5, 2013
Publication date	Mar 5, 2019
Grant date	Mar 5, 2019

Abstract

A modular analytic system includes a base, at least one fluid sample processing module configured to be removably attached to the base, at least one fluid sample analysis module configured to be removably attached to the base, a fluid actuation module positioned on the base, a fluidic network comprising multiple fluidic channels, in which the fluid actuation module is arranged to control transport of a fluid sample between the at least one sample processing module and the at least one sample analysis module through the fluidic network, and an electronic processor, in which the electronic processor is configured to control operation of the fluid actuation module and receive measurement data from the at least one fluid sample analysis module.

First claim

We claim:

1. A modular analytic system comprising:
a base;
at least one fluid sample processing module configured to be removably attached to the base;
at least one fluid sample analysis module configured to be removably attached to the base;
a fluid actuation module positioned on the base;
a fluidic network comprising a plurality of fluidic channels, wherein the fluid actuation module is arranged to control transport of a fluid sample between the at least one sample processing module and the at least one sample analysis module through the fluidic network,
wherein each fluid sample processing module and each fluid sample analysis module comprises a corresponding internal fluidic chip,
wherein each fluid sample processing module and each fluid sample analysis module comprises a corresponding plurality of tubes extending from the internal fluidic chip and out of the module to the base, at least one tube of the plurality of tubes for each module is arranged to receive a fluid sample from the fluidic network to the internal fluidic chip and at least one other tube of the plurality of tubes for each module is arranged to deliver a fluid sample from the internal fluidic chip to the fluidic network when the module is attached to the base,
wherein an underside of each fluid sample processing module and each fluid sample analysis module comprises a plurality of recesses facing the base, each recess comprising a hole through which a respective tube from the plurality of tubes extends,
wherein each fluid sample processing module and each fluid sample analysis module comprises
a corresponding first fluidic seal that forms a sealed pathway between the plurality of tubes extending out of the module and the fluidic channels of the fluidic network, each first fluidic seal comprising a gasket having a plurality of sealing rings that seat within the plurality of recesses, respectively, wherein each sealing ring of the plurality of sealing rings comprises a thickness that is greater than a depth of the recess within which the sealing ring sits, and
a corresponding second fluidic seal that is internal to the module and provides a fluidic seal between the internal fluidic chip and the plurality of tubes; and

an electronic processor, wherein the electronic processor is configured to control operation of the fluid actuation module and receive measurement data from the at least one fluid sample analysis module.

2. The modular analytic system of claim 1 , further comprising a cartridge, wherein the cartridge comprises:
one or more first receptacles configured to store a fluid sample;
one or more second receptacles configured to store one or more reagents; and
a third receptacle, wherein
the fluid sample processing module comprises an opening to receive the cartridge,
wherein the sample processing module further comprises an actuation input port and a fluid output port, wherein the actuation input port and the fluid output port are arranged to couple to the third receptacle of the cartridge when the cartridge is positioned in the opening of the sample processing module.

3. The modular analytic system of claim 2 , wherein the base comprises a first connector interface coupled to the fluid actuation module, and wherein the actuation input port of the sample processing module is configured to mate with the first connector interface.

4. The modular analytic system of claim 3 , wherein the board comprises a fluidic connector interface coupled to the fluidic network, and wherein the fluidic output port of the sample processing module is configured to mate with the fluidic connector interface.

5. The modular analytic system of claim 1 , wherein the at least one fluid sample analysis module comprises a light detector module, an electrochemistry module, a cytometry module, or a Coulter counter module.

6. The modular analytic system of claim 1 , comprising a plurality of the fluid sample analysis modules removably attached to the base, wherein at least one fluid sample analysis module is arranged to deliver a fluid sample through the fluidic network to at least one other fluid sample analysis module.

7. The modular analytic system of claim 1 , wherein the fluid actuation module comprises a compressed gas or hydraulic source to supply pressure to the fluidic network, wherein the fluid actuation module comprises a manifold that separates the pressure supplied by the fluid actuation module into a plurality of independent channels, wherein the fluid actuation module comprises a plurality of valves, and wherein the electronic processor is operable to control the operation of the plurality of valves.

8. The modular analytic system of claim 1 , wherein the fluid actuation module is removably attached to the base, wherein the fluid actuation module comprises one or more protrusions or openings, and wherein, for each protrusion or opening on the fluid actuation module, the base comprises a corresponding opening or protrusion that frictionally fits to the protrusion or opening.

9. The modular analytic system of claim 1 , further comprising a waste module configured to be removably attached to the base, wherein the waste module comprises a fluidic connector interface that mates with one or more of the fluidic channels, wherein the waste module comprises one or more protrusions or openings, wherein, for each protrusion or opening on the waste module, the base comprises a corresponding opening or protrusion that frictionally fits to the protrusion or opening, and wherein the fluidic actuation module is operable to control the flow of fluid samples from the at least one analytic module to the waste module.

10. The modular analytic system of claim 1 , wherein the fluidic channels are formed in the base or comprise a plurality of tubes.

11. The modular analytic system of claim 1 ,
wherein each sealing ring of the plurality of sealing rings comprises an outer diameter that is as wide as a diameter of the recess within which the sealing ring sits,
wherein each sealing ring of the plurality of sealing rings comprises an inner diameter corresponding to a hole within the sealing ring, and
wherein the inner diameter is as wide as an outer diameter of a tube that extends through the hole within the sealing ring.

12. The modular analytic system of claim 11 , wherein the plurality of sealing rings are connected by a membrane having a thickness that is less than the thickness of the sealing rings.

Assignees

• Massachusetts Gen Hospital

Inventors

• Granier Robert
• Haghgooie Ramin
• Kotz Ken
• Petrofsky Anne C
• Tompkins Ronald G

Classifications

• G01N2021/6493

  by alternating fluorescence/transmission or fluorescence/reflection · CPC title

• B01L2300/0832

  cylindrical, tube shaped · CPC title

• B01L2200/028

  Modular arrangements · CPC title

• G01N2015/1006

  for cytology · CPC title

• G01N21/359

  using near infrared light · CPC title