Processing microtitre plates for covalent immobilization chemistries

Patent metadata

Field	Value
Publication number	US-10220364-B2
Application number	US-201815952462-A
Country	US
Kind code	B2
Filing date	Apr 13, 2018
Priority date	Nov 17, 2009
Publication date	Mar 5, 2019
Grant date	Mar 5, 2019

Abstract

Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.

First claim

What is claimed is:

1. An article made by a method comprising:
treating a microtitre plate comprising an organic polymer and comprising a plurality of wells with an electron beam-generated plasma;
exposing the treated polymer to air or an oxygen- and hydrogen-containing gas;
wherein the treating and exposing generates hydroxyl groups on the surface of the polymer;
reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and
covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.

2. The article of claim 1 ;
wherein the reaction product is made by reacting the organosilane compound with a crosslinker; and
wherein the biomolecule is covalenlly immobilized to the crosslinker.

3. The article of claim 2 , wherein the organosilane compounds in at least two different wells are each reacted with a different crosslinker.

Assignees

• Us Navy

Inventors

• North Stella H
• Lock Evgeniya H
• Walton Scott G
• Taitt Chris Rowe

Classifications

• C08J7/16

  with polymerisable compounds · CPC title

• B01J2219/00725

  Peptides · CPC title

• B01J2219/00587

  High throughput processes · CPC title

• B01J19/0046 Primary

  Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00) · CPC title

• C08J2325/06

  Polystyrene · CPC title