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Method and compositions for improved lignocellulosic material hydrolysis
US10214758B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10214758-B2 |
| Application number | US-201213709971-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 10, 2012 |
| Priority date | Dec 22, 2011 |
| Publication date | Feb 26, 2019 |
| Grant date | Feb 26, 2019 |
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- Title
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Abstract
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A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of Streptomyces sp. ActE secretome such that at least partial lignocellulosic digestion occurs.
First claim
Opening claim text (preview).
We claim: 1. A method for digesting a non-wood biomass lignocellulosic material, wherein the method comprises: recombinantly expressing in a non-native microbial host cell SActE_0237 (GH6) (SEQ ID NO: 1), SActE_0265 (GH10) (SEQ ID NO: 5) and SActE_0236 GHQ48) (SEQ ID NO: 2), isolating SActE_0237 (GH6) (SEQ ID NO: 1), SActE_0265 (GH10) (SEQ ID NO: 5) and SActE_0236 (GH48) (SEQ ID NO: 2) from the host cell, and exposing the non-wood biomass lignocellulosic material to a sufficient amount of a composition comprising the isolated SActE_0237, SActE_0265, and SActE_0236, wherein the exposed lignocellulosic material is at least partially digested. 2. The method of claim 1 , wherein the microbial host is selected from the group consisting of Streptomyces lividans, Trichoderma reesei , and Escherichia coli. 3. The method of claim 1 , wherein the method additionally comprises recombinantly expressing in a microbial host at least one member selected from the group consisting of SActE_0357 (CE4) (SEQ ID NO:7), SActE_0358 (GH11) (SEQ ID NO:8), SActE_1310 (PL3) (SEQ ID NO:9), SActE_3717 (GH9) (SEQ ID NO:10), SActE_4638 (SEQ ID NO:11), SActE_4738 (GH16) (SEQ ID NO:12), SActE_4755 (GH64) (SEQ ID NO:13), SActE_5457 (GH46) (SEQ ID NO:14), SActE_5647 (GH87) (SEQ ID NO:15), and SActE_5978 (PL1) (SEQ ID NO:16) to produce a composition comprising isolated SActE 0237, SActE_0265, SActE_0236, and the at least one member, and exposing the at least 85% non-wood biomass lignocellulosic material to a sufficient amount of the composition comprising isolated SActE_0237, SActE_2065, SActE_0236, and the at least one member. 4. A method for digesting a non-wood biomass lignocellulosic material, wherein the method comprises: genetically modifying an ActE strain to overexpress SActE_0237 (GH6) (SEQ ID NO:1). SActE_0265 (GH10) (SEQ ID NO:5), and SActE_0236 (GH48) (SEQ ID NO:2), and exposing the non-wood biomass lignocellulosic material to the genetically modified ActE strain, wherein the exposed lignocellulosic material is at least partially digested. 5. The method of claim 4 , wherein the ActE strain is further genetically modified to overexpress least one member selected from the group consisting of SActE_0357 (CE4) (SEQ ID NO:7), SActE_0358 (GH11) (SEQ ID NO:8), SActE_1310 (PL3) (SEQ ID NO:9), SActE_3717 (GH9) (SEQ ID NO:10), SActE_4638 (SEQ ID NO:11), SActE_4738 (GH16) (SEQ ID NO:12), SActE_4755 (GH64) (SEQ ID NO:13), SActE_5457 (GH46) (SEQ ID NO:14), SActE_5647 (GH87) (SEQ ID NO:15), and SActE_5978 (PL1) (SEQ ID NO:16). 6. A method for enzymatically pretreating agricultural crop materials for consumption by ruminant animals, wherein the method comprises: recombinantly expressing in a non-native microbial host cell SActE_0237 (GH6) (SEQ ID NO:1), SActE_0265 (GH10) (SEQ ID NO:5) and SActE_0236 (GH48) (SEQ ID NO:2), harvesting said host cells, and exposing agricultural crop materials for consumption by ruminant animals to the host cells, wherein the agricultural crop materials are at least partially digested. 7. The method of claim 6 , wherein the host cell is Saccharomyces cerevisiae or Escherichia coli.
Assignees
Inventors
Classifications
Bacteria; Culture media therefor · CPC title
acting on beta-1,4-glucosidic bonds · CPC title
by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass · CPC title
Beta-mannosidase (3.2.1.25), i.e. mannanase · CPC title
Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title
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Frequently asked questions
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- What does patent US10214758B2 cover?
- A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of Streptomyces sp. ActE secretome such that at least partial lignocellulosic digestion occurs.
- Who is the assignee on this patent?
- Fox Brian Grant, Takasuka Taichi, Book Adam Joel, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12P19/14. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 26 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).