Protein assay method specific to TRACP-5b (tartrate resistant acid phosphatase 5b)

Patent metadata

Field	Value
Publication number	US-10214592-B2
Application number	US-201515505762-A
Country	US
Kind code	B2
Filing date	Aug 7, 2015
Priority date	Aug 22, 2014
Publication date	Feb 26, 2019
Grant date	Feb 26, 2019

Abstract

The purpose of the present invention is to provide a monoclonal antibody that is useful in specifically assaying tartrate resistant acid phosphatase 5b (TRACP-5b). A hybridoma producing a monoclonal antibody against TRACP-5b, said monoclonal antibody showing higher reactivity with TRACP-5b than with tartrate resistant acid phosphatase 5a (TRACP-5a) and, therefore, being specific to TRACP-5b, is obtained by cell fusion using, as an antigen, human recombinant TRACP-5b purified from silkworm silk gland. By using this monoclonal antibody, TRACP-5b in a specimen can be highly sensitively and specifically detected.

First claim

The invention claimed is:

1. A monoclonal antibody which recognizes an epitope based on a steric structure of TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b); which does not recognize any of epitopes formed of a primary structure of linear sequence of TRACP-5b; and which does not bind to TRACP-5a (Tartrate-Resistant Acid Phosphatase 5a), wherein said antibody is produced by Hybridoma TrK-126 of Accession number NITE BP-01866 or Hybridoma TrK-127 of Accession number NITE BP-01867.

2. Hybridoma TrK-126 of Accession number NITE BP-01866.

3. Hybridoma TrK-127 of Accession number NITE BP-01867.

4. A kit for use in detection of TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b), comprising the monoclonal antibody produced by Hybridoma TrK-126 of Accession number NITE BP-01866 and the monoclonal antibody produced by Hybridoma TrK-127 of Accession number NITE BP-01867; wherein each of these monoclonal antibodies recognizes an epi tope based on a steric structure of TRACP-5b;
does not recognize any of epitopes formed of a primary structure of linear sequence of TRACP-5b; and
does not bind to TRACP-5a (Tartrate-Resistant Acid Phosphatase 5a),
as constituents.

Assignees

• Nitto Boseki Co Ltd

Inventors

• Kikuchi Wataru
• Sasagawa Kumiko
• Noda Kenta

Classifications

• G01N33/53

  Immunoassay; Biospecific binding assay; Materials therefor · CPC title

• G01N33/577

  involving monoclonal antibodies {binding reaction mechanisms characterised by the use of monoclonal antibodies (G01N33/5302 - G01N33/576 take precedence)} · CPC title

• G01N33/545

  Synthetic resin · CPC title

• C07K16/30

  from tumour cells · CPC title

• C12N5/12

  Fused cells, e.g. hybridomas · CPC title