Microfluidic devices

Patent metadata

Field	Value
Publication number	US-10213782-B2
Application number	US-201615059098-A
Country	US
Kind code	B2
Filing date	Mar 2, 2016
Priority date	Mar 3, 2015
Publication date	Feb 26, 2019
Grant date	Feb 26, 2019

Abstract

In one representative embodiment, a device includes a main microchannel and at least two other microchannels. The main microchannel defines a main fluid flow path and has an opening, and first and second microchannels defining a first and second fluid flow paths, respectively. The first fluid flow path is in fluidic communication with the main fluid flow path via the opening and forms a first angle relative to the main microchannel less than 90 degrees. The second microchannel defines a second fluid flow path in fluidic communication with the main fluid flow path via the opening and in fluidic communication with the first fluid flow path. The second microchannel forms a second angle relative to the main microchannel less than 90 degrees. The first and second microchannels form a third angle relative to one another, with the third angle being between 60 and 135 degrees.

First claim

We claim:

1. A device comprising:
a main microchannel defining a main fluid flow path and having a first end portion, a second end portion, a first opening, and a second opening, wherein the first opening is disposed between the first end portion and the second opening, and wherein the second opening is disposed between the first opening and the second end portion;
a first microchannel defining a first fluid flow path, the first fluid flow path being in fluidic communication with the main fluid flow path via the second opening, the first microchannel forming a first angle relative to the main microchannel, the first angle being less than 90 degrees;
a second microchannel defining a second fluid flow path, the second fluid flow path being in fluidic communication with the main fluid flow path via the second opening and in fluidic communication with the first fluid flow path, the second microchannel forming a second angle relative to the main microchannel, the second angle being less than 90 degrees, wherein the first and second microchannel form a third angle relative to one another, the third angle being between 60 and 135 degrees;
an electric field generator having one or more microchannels positioned adjacent the main fluid flow path at the location of the second opening; and
a third microchannel defining a third fluid flow path, the third fluid flow path being in fluidic communication with the main fluid flow path via the first opening,
wherein the main fluid flow path has an oil flowing therein from the first end portion of the main microchannel toward the second end portion of the main microchannel,
wherein the main microchannel and the third microchannel are configured for forming one or more aqueous droplets in the oil flow at the first opening of the main microchannel, wherein the aqueous drops flow in the main fluid flow path from the first opening of the main microchannel toward the second end portion of the main microchannel, and
wherein the main microchannel, the first and second microchannels, and the electric field generator are configured for manipulating the volume of the aqueous droplets in the oil as the aqueous droplets and the oil pass by the second opening of the main microchannel and continue in the main fluid flow path toward the second end portion of the main microchannel.

2. The device of claim 1 , further comprising:
a main fluid control member configured to control the flow of the aqueous droplets and the oil in the main fluid flow path, wherein the oil and the aqueous droplets are a first fluid; and
at least one additional fluid control member configured to control the flow of a second fluid in the first and second fluid flow paths.

3. The device of claim 2 , wherein the main fluid control member is configured to cause the first fluid to flow along the main fluid flow path in a first direction, and wherein the additional fluid control member is configured to cause the second fluid to flow along the first and second fluid flow paths in a second direction, wherein at the second opening of the main microchannel, the second direction is generally parallel to the first direction.

4. The device of claim 2 , wherein the main fluid control member is configured to cause the first fluid to flow along the main fluid flow path in a first direction, and wherein the additional fluid control member is configured to cause the second fluid to flow along the first and second fluid flow paths in a second direction, wherein at the second opening of the main microchannel, the second direction is generally opposite to the first direction.

5. The device of claim 1 , wherein only the first and second microchannels are in fluidic communication with the second opening of the main microchannel, and wherein the main microchannel and the first and second microchannels form a K-junction at the second opening.

6. The device of claim 5 , wherein only the third microchannel is in fluidic communication with the first opening of the main microchannel, and wherein the main microchannel and the third microchannel form a T-junction at the first opening.

7. A method for manipulating droplets in a microfluidics system, the method comprising:
delivering a plurality of aqueous droplets and a first fluid along a main fluid flow path of a main microchannel, wherein one or more of the aqueous droplets has a first volume;
delivering a second fluid along a side flow path defined by intersecting first and second microchannels, the first and second microchannels forming an angle therebetween and intersecting with one another at an opening in the main microchannel; and
altering the first volume of the one or more aqueous droplets as respective aqueous droplets move along the main fluid flow path and pass the opening in the main microchannel, wherein after passing the opening, the one or more aqueous droplets continue along the main fluid flow path in the first fluid and have a second volume that is less than or greater than the first volume.

8. The method of claim 7 , wherein the act of altering the volume of the one or more aqueous droplets comprises:
removing a portion of the one or more aqueous droplets and directing the removed portion into the side flow path.

9. The method of claim 7 , wherein the act of altering the volume of the one or more aqueous droplets comprises:
increasing the volume of the one or more aqueous droplets by injecting a fluid from the side flow path into the main fluid flow path.

10. The method of claim 7 , wherein the first fluid is delivered along the main fluid flow path in a first direction and the second fluid is delivered along the side flow path in a second direction, and at the intersection of the first and second microchannels the second direction is generally parallel to the first direction.

11. The method of claim 7 , wherein the first fluid is delivered along the main fluid flow path in a first direction and the second fluid is delivered along the side flow path in a second direction, and at the intersection of the first and second microchannels the second direction is generally opposite of the first direction.

12. The method of claim 7 , further comprising:
applying an electric field to the aqueous droplets adjacent to the opening in the main microchannel.

13. A method for manipulating droplets in a microfluidics system, the method comprising:
delivering a plurality of droplets and a first fluid along a main fluid flow path of a main microchannel;
delivering a second fluid along a side flow path defined by intersecting first and second microchannels, the first and second microchannels forming an angle therebetween and intersecting with one another at an opening in the main microchannel; and
increasing the volume of one or more of the droplets by injecting a fluid from the side flow path into the main fluid flow path as respective droplets move along the main fluid flow path and pass the opening in the main microchannel.

14. A device comprising:
a main microchannel defining a main fluid flow path and having an opening;
a first microchannel defining a first fluid flow path, the first fluid flow path being in fluidic communication with the main fluid flow path via the opening, the first microchannel forming a first angle relative to the main microchannel, the first angle being less than 90 degrees;
a second microchannel defining a second fluid flow path, the second fluid flow path being in fluidic communication with the main fluid flow path via the opening and in fluidic communication with the first fluid flow path, the second microchannel forming a second angle relative to the main microchannel, the secon

Assignees

• Univ Illinois

Inventors

• Bailey Ryan C
• Doonan Steven R
• Xu Yi

Classifications

• B01L3/0241

  Drop counters; Drop formers · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title

• B01L3/502784 Primary

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• B01L2400/0415

  electrical forces, e.g. electrokinetic · CPC title

• B01L2200/0673

  Handling of plugs of fluid surrounded by immiscible fluid · CPC title