Three-component-multistage malaria vaccine

Patent metadata

Field	Value
Publication number	US-10213501-B2
Application number	US-201515509409-A
Country	US
Kind code	B2
Filing date	Sep 2, 2015
Priority date	Sep 8, 2014
Publication date	Feb 26, 2019
Grant date	Feb 26, 2019

Abstract

The technology provided herein relates to novel malaria vaccines composed of different recombinant proteins, in particular recombinant fusion proteins comprising several different Plasmodium falciparum antigens from the pre-erythrocytic, the blood, and the sexual parasite main stages. The proteins may be used in a mixture vaccine formulation to elicit protective immune responses in humans. Nucleic acid molecules encoding said recombinant proteins, vectors and host cells containing the nucleic acids and methods for preparation and producing such proteins are also disclosed, as well as antibodies induced or generated by the use of said malaria vaccines and the use of such antibodies or recombinant derivatives for passive immunotherapy.

First claim

What is claimed is:

1. A mixture of recombinant proteins as an immunogenic composition for inducing an immune response in a human against the parasite Plasmodium falciparum , wherein the mixture comprises:
a) a first recombinant fusion protein (fusion protein 1) comprising PfAMA1-DICO1, Pfs25 and PfCSP_TSR;
b) a second recombinant fusion protein (fusion protein 2) comprising PfAMA1-DICO2, Pfs25 and PfMSP1-19; and
c) a third recombinant fusion protein (fusion protein 3) comprising PfAMA1-DICO3, Pfs25 and a PfRh5 peptide.

2. The mixture according to claim 1 , wherein the recombinant fusion proteins are in equimolar ratios in the mixture.

3. The mixture according to claim 1 , further comprising an adjuvant.

4. The mixture according to claim 1 , wherein:
a) fusion protein 1 comprises the amino acid sequence of SEQ ID NO. 9;
b) fusion protein 2 comprises the amino acid sequence of SEQ ID NO. 10; and
c) fusion protein 3 comprises the amino acid sequence of SEQ ID NO. 11.

5. The mixture according to claim 1 , wherein:
a) fusion protein 1 comprises the amino acid sequence of SEQ ID NO. 9;
b) fusion protein 2 comprises the amino acid sequence of SEQ ID NO. 10; and
c) fusion protein 3 comprises the amino acid sequence of SEQ ID NO. 12.

Assignees

• Fraunhofer Ges Forschung

Inventors

• Spiegel Holger
• Boes Alexander
• Edgue Gueven
• Beiss Veronique
• Sack Markus
• Reimann Andreas
• Fischer Rainer

Classifications

• C12N15/62

  DNA sequences coding for fusion proteins · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title

• C07K14/445

  Plasmodium · CPC title

• A61K2039/70

  Multivalent vaccine · CPC title

• A61K2039/64

  characterised by the architecture of the carrier-antigen complex, e.g. repetition of carrier-antigen units · CPC title