RNA-YY1 interactions

Patent metadata

Field	Value
Publication number	US-10208305-B2
Application number	US-201214130769-A
Country	US
Kind code	B2
Filing date	Jul 3, 2012
Priority date	Jul 5, 2011
Publication date	Feb 19, 2019
Grant date	Feb 19, 2019

Abstract

Methods relating to obtaining libraries of YY1-binding long non-coding RNAs, libraries obtained thereby, and methods of use thereof.

First claim

What is claimed is:

1. A method of identifying a compound that disrupts binding of a Yin-Yang 1 (YY1)-binding long non-coding RNA (lncRNA) to Yin-Yang 1 (YY1) protein, the method comprising:
(a) identifying an lncRNA that binds YY1 protein;
(b) providing a cell-free sample comprising a YY1-binding lncRNA and YY1 protein, wherein the YY1-binding lncRNA binds to the YY1 protein thereby forming lncRNA-YY1 protein complexes;
(c) contacting the cell-free sample with a test compound, wherein the test compound is a nucleic acid targeting the YY1-binding lncRNA;
(d) isolating the lncRNA-YY1 protein complexes from the cell-free sample by contacting the cell-free sample comprising the lncRNA-YY1 protein complexes with an anti-YY1 antibody, wherein the anti-YY1 antibody specifically binds to YY1 protein, and immunoprecipitating the lncRNA-YY1 protein complexes; and
(e) detecting formation of the lncRNA-YY1 protein complexes in presence and absence of the test compound, wherein a decrease in formation of lncRNA-YY1 protein complexes in the presence of the test compound as compared to formation of lncRNA-YY1 protein complexes in the absence of test compound indicates that the test compound disrupts the binding of the YY1-binding lncRNA to the YY1 protein.

2. The method of claim 1 , wherein the YY1 protein is labeled.

3. The method of claim 1 , further comprising wherein the YY1 protein not bound to YY1-binding lncRNA is isolated from the cell-free sample.

4. The method of claim 1 , wherein the YY1-binding lncRNA is labeled.

5. The method of claim 1 , wherein both the YY1 protein and the YY1-binding lncRNA are labeled.

Assignees

• Lee Jeannie T
• Jeon Yesu
• Massachusetts Gen Hospital

Inventors

• Lee Jeannie T
• Jeon Yesu

Classifications

• A61P35/00

  Antineoplastic agents · CPC title

• G01N33/5758

  involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites · CPC title

• G01N33/575

  for cancer · CPC title

• C12N15/1048 Primary

  SELEX · CPC title

• G01N33/5011

  for testing antineoplastic activity · CPC title