Porous membrane having immobilized enzyme, porous membrane composite including the same, and preparation method thereof

Patent metadata

Field	Value
Publication number	US-10202596-B2
Application number	US-201414508414-A
Country	US
Kind code	B2
Filing date	Oct 7, 2014
Priority date	Apr 10, 2013
Publication date	Feb 12, 2019
Grant date	Feb 12, 2019

Abstract

Disclosed herein is a porous membrane having an immobilized enzyme wherein the enzyme is immobilized within pores which are three-dimensionally connected to each other. The porous membrane having the immobilized enzyme is three-dimensionally crosslinked in a molecular level wherein nanopores of 5 to 100 nm are interconnected, so that the immobilized enzyme may be in contact with a reactant in all directions, and the reaction solution may be easily diffused, thereby proceeding with the catalytic reaction fast and conveniently without deterioration of material transport.

First claim

What is claimed is:

1. A porous membrane having an immobilized enzyme, wherein
the porous membrane is three-dimensionally interconnected by pores, the pores have a size of 5 nm to 100 nm,
a framework of the porous membrane is a three-dimensional network formed by polymerization of a first monomer and a second monomer, wherein the first monomer is tetrakis(4-aminophenyl) methane (TAPM), and the second monomer is hexamethylene diisocyanate (HDI), and
the three-dimensional network is formed by polymerization occurring by reacting the first monomer with the second monomer.

2. The porous membrane of claim 1 , wherein the porous membrane has a thickness of 10 μm to 100 μm.

3. The porous membrane of claim 1 , wherein the porous membrane has a structure of a flat membrane or a hollow fiber membrane.

4. The porous membrane of claim 1 , wherein the enzyme is one or more selected from the group consisting of lipase, amylase, protease, trypsin, papain, brinase, peroxidase, horseradish peroxidase (HRP), carbonic anhydrase, aquaporin, chymotrypsin, subtilisin, soybean peroxidase, chloroperoxidase, manganese peroxidase, tyrosinase, laccase, cellulase, xylanase, lactase, sucrase, organophosphohydrolase, chlorinesterase, glucose oxidase, alcohol dehydrogenase, glucose dehydrogenase, hydrogenase, and glucose isomerase.

5. A multiple layered membrane having a structure wherein two or more of the porous membranes of claim 1 are overlaid.

Assignees

• Gwangju Inst Science & Tech

Inventors

• Park Ji-Woong
• Han Sun-Young
• Bae Jae-Sung

Classifications

• C12N11/08

  the carrier being a synthetic polymer · CPC title

• B01D71/56

  Polyamides, e.g. polyester-amides · CPC title

• B01D71/64

  Polyimides; Polyamide-imides; Polyester-imides; Polyamide acids or similar polyimide precursors · CPC title

• C12P13/00

  Preparation of nitrogen-containing organic compounds · CPC title

• C12P7/649

  Biodiesel, i.e. fatty acid alkyl esters · CPC title