Stable liquid lactase compositions

Patent metadata

Field	Value
Publication number	US-10201178-B2
Application number	US-201515122625-A
Country	US
Kind code	B2
Filing date	Mar 5, 2015
Priority date	Mar 5, 2014
Publication date	Feb 12, 2019
Grant date	Feb 12, 2019

Abstract

A stable aqueous liquid lactase formulation is provided, comprising lactase and further comprising sodium, calcium or potassium- L -lactate or a combination thereof and optionally a sugar, and/or optionally comprising sodium or potassium chloride or a combination thereof, preferably wherein the concentration of each of the components is such that the water activity A w is at most 0.82. The formulation is particularly suitable when using invertase-free lactase, allowing the use of sucrose as stabilizer. Also provided is a process to produce the liquid lactase formulation, an infant formula (e.g. as powder of granulate) comprising the liquid lactase formulation, a method to produce said infant formula, and the use of the formulation in the production of infant formula.

First claim

The invention claimed is:

1. A stable aqueous liquid lactase formulation comprising lactase and at least 20 wt % sodium- L -lactate, calcium- L -lactate, potassium- L -lactate, or a combination thereof, wherein the concentration of each of the components is such that the water activity A w is at most 0.82, and wherein said formulation is substantially free of polyols or diols, including glycerol.

2. The stable aqueous liquid lactase formulation according to claim 1 , further comprising a sugar.

3. The stable aqueous liquid lactase formulation according to claim 1 , further comprising sodium chloride, or potassium chloride, or a combination thereof.

4. The stable aqueous liquid lactase formulation according to claim 2 wherein said sugar is sucrose, fructose, glucose, lactose, or a combination thereof.

5. The stable aqueous liquid lactase formulation according to claim 1 wherein the lactase is a neutral lactase.

6. The stable aqueous liquid lactase formulation according to claim 1 wherein the aqueous liquid lactase formulation is substantially free of invertase.

7. The stable aqueous liquid lactase formulation according to claim 6 which further comprises sucrose.

8. The stable aqueous liquid lactase formulation of claim 1 , further comprising whey protein.

9. The stable aqueous liquid lactase formulation of claim 1 which is further substantially free of sorbitol.

10. An infant, follow-on and/or toddler formula as powder of granulate, comprising the stable aqueous liquid lactase formulation of claim 1 .

11. The infant, follow-on and/or toddler formula of claim 10 , which is a dry formulation.

12. The infant, follow-on and/or toddler formula of claim 10 , wherein lactose is present in an amount of less than 1 wt %, based on the total weight of the infant, follow-on or toddler formula.

13. A process to produce the stable aqueous liquid lactase formulation of claim 1 said process comprising adding and mixing:
(a) lactase,
(b) sodium- L -lactate, calcium- L -lactate, potassium- L -lactate, or a combination thereof, and
(c) water.

14. The process of claim 13 , further comprising adding and mixing:
(d) sugar, sodium chloride, potassium chloride, or a combination thereof.

15. A method to produce an infant, follow-on or toddler formula comprising:
(a) adding the stable aqueous liquid lactase formulation of claim 1 to a milk concentrate;
(b) mixing; or homogenizing;
(c) evaporating; and
(d) spray-drying.

16. The method of claim 15 , further comprising granulating.

17. The method of claim 15 , further comprising adding minerals, vitamins, fats, or combinations thereof to: the stable aqueous liquid lactase formulation; the milk concentrate; or the milk concentrate to which the stable aqueous liquid lactase formulation has been added.

18. A stable aqueous liquid lactase formulation consisting essentially of
(i) lactase and at least 20 wt % sodium- L -lactate, calcium- L -lactate,-potassium- L -lactate, or a combination thereof,
(ii) sugar; and
(iii) whey protein,
(iii) sodium chloride, or potassium chloride, or a combination thereof,
wherein the concentration of each of the components is such that the water activity A w is at most 0.82, wherein said formulation is substantially free of polyols or diols, including glycerol, and wherein the formulation is suitable for administration to infants and toddlers.

19. An infant, follow-on and/or toddler formula as powder of granulate, comprising the stable aqueous liquid lactase formulation of claim 18 .

20. The stable aqueous liquid lactase formulation according to claim 19 wherein said sugar is sucrose, fructose, glucose, lactose, or a combination thereof.

Assignees

• Dsm Ip Assetss B V
• Dsm Ip Assets Bv

Inventors

• Jolink Fenna Johanna Catharina
• Schaap Albert

Classifications

• C12Y302/01108

  Lactase (3.2.1.108) · CPC title

• A23L2/66 Primary

  Proteins · CPC title

• C12N9/2468

  acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81) · CPC title

• A23L33/40 Primary

  Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title