Method for treating fibrosis using siRNA and a retinoid-lipid drug carrier

Patent metadata

Field	Value
Publication number	US-10195145-B2
Application number	US-201615283179-A
Country	US
Kind code	B2
Filing date	Sep 30, 2016
Priority date	Jun 8, 2011
Publication date	Feb 5, 2019
Grant date	Feb 5, 2019

Abstract

What is described is a method for treating a fibrotic disease by administering a pharmaceutical composition comprising a drug carrier, which comprises a lipid and a retinoid, and a double-stranded nucleic acid molecule, which comprises an antisense sequence to mRNA encoding human hsp47.

First claim

What is claimed:

1. A method for treating fibrosis in a subject in need thereof, the method comprising administering an effective amount of a pharmaceutical composition to the subject, wherein the pharmaceutical composition comprises a drug carrier and a double-stranded nucleic acid molecule, wherein the drug carrier comprises a cationic lipid and a retinoid molecule, and wherein the double-stranded nucleic acid molecule is a modified nucleic acid molecule having a structure (A2) set forth below:

(A2)

5′ N 1 -(N) x -Z 3′
(antisense strand)

3′ Z′-N 2 -(N′) y -z″ 5′
(sense strand)

wherein each of (N) 2 and N′ is an unmodified or modified nucleotide, or an unconventional moiety;
wherein each of (N) x and (N′) y is an oligonucleotide in which each consecutive N or N′ is joined to the adjacent N or N′ by a covalent bond, and wherein (N) x comprises at least one modified ribonucleotide among a multiplicity of unmodified or modified ribonucleotides, or unconventional moieties, N;
wherein each of x and y is independently an integer between 17 and 39;
wherein the sequence of (N′) y has complementarity to the sequence of (N) x and (N) x comprises an antisense sequence to the mRNA coding sequence for human hsp47 exemplified by SEQ ID NO:1;
wherein N 1 is covalently bound to (N) x and is mismatched to the target RNA or is a complementary DNA moiety to the target RNA;
wherein N 2 is covalently bound to (N′)y and forms a base pair with N 1 ;
wherein each of Z and Z′ is independently present or absent, but if present is independently 1-5 consecutive nucleotides, consecutive non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present;
wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N 2 -(N′) y ; and
wherein N 1 is a moiety selected from the group consisting of natural or modified uridine, deoxyribouridine, ribothymidine, deoxyribothymidine, adenosine or deoxyadenosine.

2. The method of claim 1 , wherein the cationic lipid is a molecule with the structure

3. The method of claim 1 , wherein the cationic lipid is a molecule with the structure

4. The method according to claim 1 , wherein the pharmaceutical formulation is parenterally administered.

5. The method of claim 1 , wherein the fibrosis is liver fibrosis.

6. The method of claim 5 , wherein the liver fibrosis is selected from the group consisting of non-alcoholic steatohepatitis (NASH); hepatitis; hepatic fibrosis; chronic hepatitis C virus (HCV) infection; hepatic cirrhosis; chronic hepatic damage; and liver cancer.

7. The method of claim 1 , wherein the fibrosis is pulmonary fibrosis.

8. The method of claim 7 , wherein the fibrosis is selected from the group consisting of pulmonary fibrosis including idiopathic pulmonary fibrosis, interstitial lung disease, radiation pneumonitis leading to pulmonary fibrosis; and fibrotic lung disease.

9. The method of claim 1 , wherein the fibrosis is kidney fibrosis.

10. The method of claim 9 , wherein the kidney fibrosis is chronic renal failure or diabetic nephropathy.

11. The method of claim 1 , wherein the fibrosis is peritoneal fibrosis.

12. The method of claim 11 , wherein the peritoneal fibrosis is peritoneal sclerosis.

13. The method of claim 1 , wherein the fibrosis is pancreatic fibrosis.

14. The method of claim 13 , wherein the pancreatic fibrosis is pancreatitis, pancreatic fibrosis, or pancreatic cancer.

15. The method of claim 1 , wherein the fibrosis is cardiac fibrosis.

16. The method of claim 15 , wherein the cardiac fibrosis is myocardial fibrosis.

17. The method of claim 1 , wherein the fibrosis is intestinal fibrosis.

18. The method of claim 1 , wherein the fibrosis is fibrosis of the eye.

19. The method of claim 18 , wherein the fibrosis of the eye is selected from the group consisting of ocular scarring including proliferative vitreoretinopathy (PVR) and scarring resulting from surgery to treat cataract or macular degeneration, glaucoma, Graves' ophthalmopathy; ocular cicatricial pemphigoid, and drug induced ergotism.

20. The method of claim 1 , wherein the fibrosis is skin fibrosis.

21. The method of claim 20 , wherein the skin fibrosis including scleroderma, keloids and hypertrophic scars scleroderma; psoriasis; and Kaposi's sarcoma.

22. The method of claim 1 , wherein the fibrosis is fibrosis of bone.

23. The method of claim 1 , wherein the fibrosis is selected from the group consisting of myelofibrosis; myeloid leukemia; acute myelogenous leukemia; myelodysplastic syndrome; and myeloproliferative syndrome.

24. The method of claim 1 , wherein the fibrosis is inflammatory bowel disease of variable etiology.

25. The method of claim 1 , wherein the fibrosis is glioblastoma in Li-Fraumeni syndrome or sporadic glioblastoma.

26. The method of claim 1 , wherein the fibrosis is gynecological cancer.

27. The method of claim 1 , wherein the fibrosis is Hansen's disease.

28. The method of claim 1 , wherein the fibrosis is collagenous colitis.

29. The method of claim 1 , wherein the fibrosis is fibrillogenesis.

30. The method of claim 1 , wherein the fibrosis is fibrosis of vocal cords.

31. The method of claim 30 , wherein the fibrosis is selected from the group consisting of vocal cord scarring, vocal cord mucosal fibrosis, and laryngeal fibrosis.

Assignees

• Nitto Denko Corp

Inventors

• Niitsu Yoshiro
• Knopov Victor
• Payne Joseph E
• Witte Richard P
• Ahmadian Mohammad
• Perelman Loren A
• Akopian Violetta
• Tanaka Yasunobu
• Feinstein Elena
• Avkin-Nahum Sharon
• Kalinski Hagar
• Mett Igor
• Minomi Kenjiro
• Ying Wenbin
• Liu Yun

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• A61P31/14

  for RNA viruses · CPC title

• A61P27/02

  Ophthalmic agents · CPC title