Rationale, methods, and assays for identifying human and non-human primate taste specific genes and use thereof in taste modulator and therapeutic screening assays

Patent metadata

Field	Value
Publication number	US-10191050-B2
Application number	US-201715681755-A
Country	US
Kind code	B2
Filing date	Aug 21, 2017
Priority date	Jun 8, 2007
Publication date	Jan 29, 2019
Grant date	Jan 29, 2019

Abstract

This invention relates to novel rationale and methods for identifying human and primate taste-specific genes, including genes involved in salty taste perception, especially human salty taste perception, but also genes involved in sweet, bitter, umami, and sour taste perception, and genes involved in other taste cell or taste receptor related activities such as digestive function and digestive related diseases, taste cell turnover, immunoregulation of the oral and digestive tract, and metabolic regulation such as in diabetes and obesity, the genes identified using these methods, and assays for identifying taste modulators (enhancers or blockers) and potential therapeutics using these genes. These compounds have potential application in modulating (enhancing or blocking) taste perception, especially salty taste perception and as potential therapeutics. In addition, this invention relates to novel methods for identifying taste-specific genes that can be used as markers for different taste cell types, including sweet, bitter, umami, sour, salty, and other taste cells in mammals as well as assays that measure the activity of the sweet, bitter, umami, or sour receptor in the presence of these genes to identify modulators of sweet, bitter, umami, and sour taste and to identify therapeutics especially for treating digestive or metabolic disorders, taste loss, and oral infections. Particularly, the genes identified herein and antibodies or oligos thereto can be used as markers to identify and/or purify specific taste cells e.g., from taste cell suspensions by use of FACS or magnetic bead cell selection or other known cell purification and isolation procedures.

First claim

The invention claimed is:

1. A method of identifying from a population of T1R expressing cells those which only express T1R3 and not T1R2 or T1R3 comprising (i) detecting whether said T1R expressing cells express LOC285965 and (ii) if positive identifying said cells as cells which express T1R3 and not T1R2 or T1R3.

2. The method of claim 1 , wherein said identified cells which express T1R3 and not T1R2 or T1R3 are isolated.

3. The method of claim 1 , wherein LOC285965 expression is detected using an anti-LOC285965 antibody.

4. The method of claim 1 , wherein said T1R expressing cells are human or non-human primate.

Assignees

• Senomyx Inc

Inventors

• Moyer Bryan
• Zlotnik Albert
• Hevezi Peter
• Soto Hortensia
• Kalabat Dalia
• Lu Min
• Gao Na
• White Evan Carl

Classifications

• G01N2333/726

  G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH · CPC title

• C40B40/10

  Libraries containing peptides or polypeptides, or derivatives thereof · CPC title

• C12Q1/6876

  Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes · CPC title

• G01N33/566 Primary

  using specific carrier or receptor proteins as ligand binding reagents {where possible specific carrier or receptor proteins are classified with their target compounds} · CPC title

• C12Q2600/136

  Screening for pharmacological compounds · CPC title