Sorting flow cytometer

Patent metadata

Field	Value
Publication number	US-10190959-B2
Application number	US-201314377623-A
Country	US
Kind code	B2
Filing date	Feb 8, 2013
Priority date	Feb 9, 2012
Publication date	Jan 29, 2019
Grant date	Jan 29, 2019

Abstract

A sorting flow cytometer identifies an undesirable drop charge sequence that is preassigned to adjacent drops before the drops have separated from a fluid stream. An example of an undesirable drop charge sequence is a sequence of adjacent drops that are charged with sufficiently high opposing charges that, after the drops are formed, would result in merging of the adjacent drops. The sorting flow cytometer adjusts the assignment of drop charges to avoid the undesired drop charge sequence.

First claim

What is claimed is:

1. A sorting flow cytometer comprising:
a fluid nozzle configured to receive a fluid input and generate a fluid stream along a fluid path;
a laser configured to generate a laser beam and positioned to direct the laser beam at the fluid stream;
acquisition electronics including a sensor analyzer arranged and configured to detect light from the laser after the laser beam has intersected the fluid stream;
a computing device in data communication with the acquisition electronics;
a computer readable data storage device storing data instructions that, when executed by the computing device, cause the computing device to perform the following steps:
identify segments of the fluid stream, including a first segment and a second segment that are directly adjacent to each other;
generate preliminary sort decisions that assign preliminary charges to segments of the fluid stream including a first preliminary sort decision that assigns a first preliminary charge to the first segment and a second preliminary sort decision that assigns a second preliminary charge to the second segment;
evaluate the first preliminary sort decision and the second preliminary sort decision;
generate final sort decisions that include a modification to the first preliminary sort decision or the second preliminary sort decision to assign a neutral charge for at least one of the first segment or the second segment; and
generate a sort control signal according to the final sort decisions;

sort control electronics in data communication with the computing device, configured to receive the sort control signal from the computing device and selectively apply charges to the segments of the fluid stream prior to a drop break off point; and
deflection plates arranged adjacent to the fluid path to sort drops formed from the segments of the fluid stream into a plurality of containers.

2. The sorting flow cytometer of claim 1 , wherein the computer readable data storage device stores data instructions that, when executed by the computing device, cause the computing device to determine if a sequence of preliminary charges assigned to adjacent segments of the fluid stream matches a sequence of charges in a lookup table.

3. The sorting flow cytometer of claim 1 ,
wherein said sorting flow cytometer is a six-way sorting cytometer,
wherein the preliminary sort decisions are associated with relative charges of +/−a, +/−b, or +/−c, wherein a, b, and c represent magnitudes, a<b<c, and +/− represent polarities; and
wherein the computer readable data storage device stores data instructions that, when executed by the computing device, cause the computing device to generate final sort decisions by modifying the preliminary sort decisions to assign a neutral charge for at least one of the adjacent segments of the fluid stream if the combination of preliminary charges is one of +c and −b; +c and −c; and +b and −c.

4. The sorting flow cytometer of claim 1 , wherein the computer readable data storage device stores data instructions that, when executed by the computing device, cause the computing device to determine whether adjacent segments of the fluid stream are assigned preliminary charges of opposite polarities that exceed a predetermined threshold value.

5. The sorting flow cytometer of claim 1 , wherein the computer readable data storage device stores data instructions that, when executed by the computing device, cause the computing device to calculate the differential between preliminary charges assigned to adjacent segments of the fluid stream.

6. The sorting flow cytometer of claim 1 , wherein the computer readable data storage device stores data instructions that, when executed by the computing device, cause the computing device to evaluate the preliminary sort decisions of adjacent segments of the fluid stream by utilizing a sliding window to evaluate a portion of the preliminary sort decisions at a time.

7. The sorting flow cytometer of claim 1 , wherein the final sort decision is based on the relative locations of the containers within the plurality of containers.

Assignees

• Beckman Coulter Inc

Inventors

• Thrasher Thomas L
• Bailey Bruce G
• Von Seggern Eric
• Degeal Jeffrey W

Classifications

• G01N35/08

  using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis · CPC title

• G01N2015/1043

  Physics · mapped topic

• G01N2015/149

  Physics · mapped topic

• G01N2015/1481

  Optical analysis of particles within droplets (sorting particles within droplets G01N15/1492) · CPC title

• G01N15/1425 Primary

  using an analyser being characterised by its control arrangement · CPC title