Maize event DP-004114-3 and methods for detection thereof

Patent metadata

Field	Value
Publication number	US-10190179-B2
Application number	US-201715706103-A
Country	US
Kind code	B2
Filing date	Sep 15, 2017
Priority date	Dec 17, 2009
Publication date	Jan 29, 2019
Grant date	Jan 29, 2019

Abstract

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

First claim

What is claimed is:

1. A method of detecting the presence of DNA corresponding to the DP-004114-3 event in a sample, the method comprising:
(a) contacting the sample comprising maize DNA with a polynucleotide probe that hybridizes under stringent hybridization conditions with DNA from maize event DP-004114-3 and does not hybridize under said stringent hybridization conditions with a non-DP-004114-3 maize plant DNA;
(b) subjecting the sample and probe to stringent hybridization conditions; and
(c) detecting hybridization of the probe to the DNA;

wherein detection of hybridization indicates the presence of the DP-004114-3 event.

2. The method of claim 1 , wherein the probe comprises the 5′ junction sequence of event DP-004114-3.

3. The method of claim 1 , wherein the probe comprises the sequence of SEQ ID NO: 27.

4. The method of claim 1 , wherein the probe comprises the 3′ junction sequence of event DP-004114-3.

5. The method of claim 1 , wherein the probe comprises the sequence of SEQ ID NO: 28.

Assignees

• Pioneer Hi Bred Int
• Du Pont
• Du Pont

Inventors

• Diehn Scott
• Lu Albert L
• Nowatzki Timothy M
• Nubel Douglas S
• Pascual M Alejandra
• Register Iii James C
• Scelonge Christopher J
• Young Joshua K
• Zhong Cathy Xiaoyan
• Crowgey Erin L

Classifications

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• A01H1/02

  Methods or apparatus for hybridisation; Artificial pollination {; Fertility} · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title