Antidotes for factor Xa inhibitors and methods of using the same

Patent metadata

Field	Value
Publication number	US-10190111-B2
Application number	US-201715435086-A
Country	US
Kind code	B2
Filing date	Feb 16, 2017
Priority date	Sep 28, 2007
Publication date	Jan 29, 2019
Grant date	Jan 29, 2019

Abstract

The present invention relates antidotes to anticoagulants targeting factor Xa. The antidotes are factor X and factor Xa protein derivatives that bind to the factor Xa inhibitors thereby substantially neutralizing them but do not assemble into the prothrombinase complex. The derivatives describe herein lack or have reduced intrinsic coagulant activity. Disclosed herein are methods of reversing anticoagulation, stopping or preventing bleeding in a patient that is currently undergoing anticoagulant therapy with a factor Xa inhibitor.

First claim

The invention claimed is:

1. An isolated two-chain polypeptide comprising:
1) a first chain comprising amino acid residues 1-105 of SEQ ID NO: 22, and
2) a second chain comprising amino acid residues 112-365 of SEQ ID NO: 22,
wherein the two-chain polypeptide (a) has reduced catalytic activity as compared to the corresponding wild-type factor Xa protein, (b) is capable of binding to a factor Xa inhibitor and (c) cannot assemble into a prothrombinase complex.

2. The isolated two-chain polypeptide of claim 1 , comprising an amino acid residue that is modified to be different from natural amino acids.

3. A pharmaceutical composition comprising a carrier and a polypeptide of claim 1 .

4. A method of neutralizing or reversing anticoagulation in a subject undergoing anticoagulant therapy with a factor Xa inhibitor in need of cessation of anticoagulation or suffering from bleeding, comprising administering to the subject an effective amount of the polypeptide of claim 1 , wherein the factor Xa inhibitor inhibits factor Xa's catalytic activity.

5. The method of claim 4 , wherein the factor Xa inhibitor is a direct factor Xa inhibitor.

6. The method of claim 4 , wherein the factor Xa inhibitor is an indirect, antithrombin III-dependent, factor Xa inhibitor.

7. The method of claim 4 , wherein the factor Xa inhibitor is selected from the group consisting of fondaparinux, idraparinux, biotinylated idraparinux, enoxaparin, fragmin, NAP-5, rNAPc2, tissue factor pathway inhibitor, DX-9065a, YM-60828, YM-150, apixaban, rivaroxaban, PD-348292, otamixaban, DU-176b, LY517717, GSK913893, razaxaban, low molecular weight heparin, betrixaban or a pharmaceutically acceptable salt thereof, and combinations thereof.

8. The method of claim 4 , wherein the administration comprises an intravenous injection.

9. The method of claim 8 , wherein the administration comprises a bolus intravenous injection.

10. The method of claim 9 , wherein the administration further comprises a continuous infusion.

Assignees

• Portola Pharm Inc

Inventors

• Sinha Uma
• Lu Genmin
• Conley Pamela B

Classifications

• A61P7/02

  Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors · CPC title

• A61P7/04

  Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents · CPC title

• A61K38/482

  Serine endopeptidases (3.4.21) · CPC title

• C12N9/6432 Primary

  Coagulation factor Xa (3.4.21.6) · CPC title

• A61K47/60

  the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol · CPC title