Artificial ovary
US-2017369851-A1 · Dec 28, 2017 · US
Artificial oocyte activation
US10190093B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10190093-B2 |
| Application number | US-201715726672-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 6, 2017 |
| Priority date | Nov 27, 2013 |
| Publication date | Jan 29, 2019 |
| Grant date | Jan 29, 2019 |
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Abstract
Official abstract text for this publication.
The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn 2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca 2+ in the oocyte prior to reduction of the intracellular Zn 2+ concentration. The invention further provides unfertilized oocytes activated by the disclosed methods and viable mammalian animals produced from unfertilized oocytes activated by the disclosed methods.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of activating an unfertilized porcine oocyte comprising decreasing intracellular Zn 2+ concentration of the oocyte by contacting the oocyte with a Zn 2+ binding moiety comprising approximately 200 mM TPEN (N,N,N′,N′-tetrakis(2-pyridylmethyl)ethane-1,2-diamine), and increasing intracellular Ca 2+ concentration of the oocyte prior to decreasing the intracellular Zn 2+ concentration of the oocyte, wherein the intracellular Ca 2+ concentration of the oocyte is not increased in an amount sufficient to induce oocyte activation, and wherein said contacting results in activating the oocyte. 2. The method of claim 1 , wherein the intracellular Ca 2+ concentration of the oocyte is increased approximately ten times less than the amount sufficient to induce oocyte activation. 3. The method of claim 1 , wherein the Zn 2+ binding moiety contacts the oocyte for a period of time comprising between approximately 30 minutes to approximately 2.5 hours. 4. The method of claim 3 , wherein the Zn 2+ binding moiety contacts the oocyte for a period of time comprising approximately 30 minutes. 5. The method of claim 1 , wherein the intracellular Ca 2+ concentration of the oocyte is increased by chemical or physical means or a combination thereof that induce calcium entry into the oocyte or release of internal calcium stores. 6. The method of claim 1 , wherein the unfertilized livestock porcine oocyte is a nuclear transfer oocyte.
Assignees
Inventors
Classifications
Cell cycle regulated proteins, e.g. cyclins, cyclin-dependant kinases · CPC title
Cells produced using nuclear transfer · CPC title
Swine · CPC title
- C12N5/0609Primary
Oocytes, oogonia (fertilised oocytes C12N5/0604) · CPC title
Cloned vertebrates · CPC title
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- What does patent US10190093B2 cover?
- The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn 2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca 2+ in the oocyte prior to reduction of the intracellular Zn 2+ concen…
- Who is the assignee on this patent?
- Lee Kiho, Prather Randall S, Univ Missouri
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0609. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 29 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).