Maize plants with improved pathogen resistance

Patent metadata

Field	Value
Publication number	US-10188052-B2
Application number	US-201514811697-A
Country	US
Kind code	B2
Filing date	Jul 28, 2015
Priority date	Aug 28, 2014
Publication date	Jan 29, 2019
Grant date	Jan 29, 2019

Abstract

The present invention provides methods and compositions for producing elite lines of corn exhibiting improved resistance to TARSC. Also provided in the present invention are corn plants exhibiting TARSC resistance resulting from such methods, and methods for breeding corn such that the TARSC resistance traits may be transferred to a desired genetic background.

First claim

What is claimed is:

1. A method of obtaining a corn plant with improved tar spot complex (TARSC) resistance, said method comprising:
a) providing a population of corn plants;
b) obtaining at least one DNA sample from at least one plant within said population;
c) detecting in the DNA sample the presence of a TARSC resistance allele comprising a “T” at nucleotide position 61 of SEQ ID NO: 6 in, or genetically linked to, a chromosomal segment between about 3.99 cM and about 17.7 cM on chromosome 10;
d) selecting one or more plants from said population based on the presence of said allele;
e) crossing at least one selected plant comprising said allele with a second corn plant that comprises one or zero TARSC resistance alleles to produce one or more progeny plants that produce seeds;
f) collecting the seeds produced by the one or more progeny plants; and
g) growing from said seeds at least one plant having said allele and having improved TARSC resistance.

2. The method of claim 1 , wherein said segment is flanked by loci SEQ ID NO: 1 and SEQ ID NO: 7 on chromosome 10.

3. The method of claim 2 , wherein said segment is flanked by loci SEQ ID NO: 4 and SEQ ID NO: 6 on chromosome 10.

4. The method of claim 1 , wherein said segment is located between about 9.4 cM and about 13.7 cM, or between about 8.3 cM and about 11.9 cM on chromosome 10.

5. The method of claim 1 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 1.

6. The method of claim 1 , wherein said plant exhibits increased yield relative to a control plant not comprising said TARSC resistance allele.

7. The method of claim 1 , wherein step (a) of providing comprises crossing a first corn plant comprising a TARSC resistance allele with a second corn plant to produce a population of corn plants.

8. The method of claim 7 , wherein producing the population of corn plants comprises backcrossing.

9. The method of claim 1 , wherein step (c) of detecting comprises the use of an oligonucleotide probe.

10. A method of producing a corn plant with improved tar spot complex (TARSC) resistance, said method comprising:
a) crossing a first corn plant comprising a TARSC resistance allele with a second corn plant of a different genotype to produce one or more progeny plants;
b) obtaining at least one DNA sample from at least one progeny plant;
c) detecting the presence of said allele comprising a “T” at nucleotide position 61 of SEQ ID NO: 6 in, or genetically linked to, a chromosomal segment between about 3.99 cM and about 17.7 cM on chromosome 10 in the DNA sample;
d) selecting one or more progeny plants based on the presence of said allele; wherein said allele confers improved TARSC resistance compared to a plant lacking said allele e) collecting seeds produced by the one or more progeny plants; and
f) growing from said seeds at least one plant having said allele and having improved TARSC resistance.

11. The method of claim 10 , wherein said segment is flanked by loci SEQ ID NO: 1 and SEQ ID NO: 7 on chromosome 10.

12. The method of claim 11 , wherein said segment is flanked by loci SEQ ID NO: 4 and SEQ ID NO: 6 on chromosome 10.

13. The method of claim 10 , wherein said segment is located between about between about 9.4 cM and about 13.7 cM, or between about 8.3 cM and about 11.9 cM on chromosome 10.

14. The method of claim 10 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 1.

15. The method of claim 10 , wherein said plant exhibits increased yield relative to a control plant not comprising said TARSC resistance allele.

16. The method of claim 10 , further comprising:
g) crossing said progeny plant with itself or a second plant to produce one or more further progeny plants; and
h) selecting a further progeny plant comprising said allele.

17. The method of claim 16 , wherein step (h) of selecting comprises marker-assisted selection.

18. The method of claim 17 , wherein said marker-assisted selection comprises selecting a progeny plant based on the presence of said allele in the sequence of SEQ ID NO:6.

19. The method of claim 16 , wherein said further progeny plant is an F2-F7 progeny plant.

20. The method of claim 19 , wherein producing the progeny plant comprises backcrossing.

21. The method of claim 20 , wherein backcrossing comprises from 2-7 generations of backcrosses.

22. The method of claim 20 , wherein backcrossing comprises marker-assisted selection.

23. The method of claim 22 , wherein backcrossing comprises marker-assisted selection in at least two generations.

24. The method of claim 23 , wherein backcrossing comprises marker-assisted selection in all generations.

25. The method of claim 22 , wherein said marker-assisted selection comprises selecting a progeny plant based on the presence of said allele in a polymorphic locus that comprises the sequence of SEQ ID NO: 1.

26. The method of claim 10 , wherein said first corn plant is an inbred or a hybrid.

27. The method of claim 10 , wherein said second corn plant is an agronomically elite corn plant.

28. The method of claim 27 , wherein said agronomically elite corn plant is an inbred or a hybrid.

29. The method of claim 1 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 2.

30. The method of claim 1 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 3.

31. The method of claim 1 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 4.

32. The method of claim 1 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 5.

33. The method of claim 1 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 6.

34. The method of claim 1 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 7.

35. The method of claim 1 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 36.

36. The method of claim 10 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 2.

37. The method of claim 10 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 3.

38. The method of claim 10 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 4.

39. The method of claim 10 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 5.

40. The method of claim 10 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 6.

41. The method of claim 10 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 7.

42. The method of claim 10 , wherein said chromosomal segment comprises the sequence of SEQ ID NO: 36.

43. The method of claim 22 , wherein said marker-assisted selection comprises selecting a progeny plant based on the presence of said allele in a polymorphic locus that comprises the sequence of SEQ ID NO: 2.

44. The method of claim 22 , wherein said marker-assisted selection comprises selecting a progeny plant based on the presence of said allele in a polymorphic locus that comprises the sequence of SEQ ID NO: 3.

45. The method of claim 22 , wherein said marker-assisted

Assignees

• Monsanto Technology Llc

Inventors

• Garza Isidro Alvarez
• Fouquet Romain Jean-Baptiste
• Jia Hongwu
• Pan Yule

Classifications

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q1/68

  involving nucleic acids · CPC title

• A01H5/00

  Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy · CPC title

• C12Q2600/13

  Plant traits · CPC title