Soybean event SYHT0H2 and compositions and methods for detection thereof

Patent metadata

Field	Value
Publication number	US-10184134-B2
Application number	US-201113994145-A
Country	US
Kind code	B2
Filing date	Dec 9, 2011
Priority date	Dec 15, 2010
Publication date	Jan 22, 2019
Grant date	Jan 22, 2019

Abstract

Soybean plants comprising event SYHT0H2, methods of detecting and using the same, and soybean plants comprising a heterologous insert at the same site as SYHT0H2.

First claim

What is claimed is:

1. A seed of a transgenic soybean plant comprising herbicide tolerance event SYHT0H2, wherein said seed comprises herbicide tolerance event SYHT0H2 and a representative sample of said seed comprising said event has been deposited under ATCC Accession No. PTA-11226.

2. A transgenic herbicide tolerance event SYHT0H2 soybean plant, cells or tissues thereof comprising a nucleic acid molecule comprising nucleotides 793-8764 of SEQ ID NO: 10.

3. A seed of a transgenic soybean plant of claim 2 , wherein said seed comprises herbicide tolerance event SYHT0H2 and further comprises one or more additional regions encoding a polypeptide providing the plant with resistance or tolerance to one or more additional herbicides, or one or more insects, fungal, bacterial and/or viral infections.

4. The transgenic soybean seed of claim 3 , wherein the one or more additional regions encode a polypeptide providing the plant with resistance or tolerance to one or more additional herbicides selected from group consisting of: glyphosate, glufosinate, dicamba, 2,4-D, phenoxy auxin herbicides, PPO herbicides, aryloxyphenoxypropionate herbicides, imidazolinone, sulfonyl urea, ametryne, triazine herbicides and metribuzin.

5. The transgenic soybean seed of claim 3 , wherein the one or more additional regions encode a polypeptide providing the plant with resistance or tolerance to one or more additional herbicides, the polypeptide being selected from the group consisting of: a glyphosate tolerant 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), glyphosate N-acetyl transferase (GAT), herbicide tolerant 4-hydroxypyruvyldioxgenase (HPPD), phosphinothricin actetyl transferase (PAT), cytochrome P450, glutathione S-transferase (GST), herbicide tolerant acetyl-COA-carboxylase (ACCase), herbicide tolerant acetolactate synthase (ALS), herbicide tolerant protoporphyrinogen oxidase (PPO), bromoxynil nitrilase, herbicide tolerant phytoene desaturase, aryloxyalkanoate dioxygenase, homogentisate solanesyltransferase (HST) and dicamba degrading enzymes.

6. The transgenic soybean seed of claim 3 , wherein the plant comprises resistance to a combination of an HPPD inhibitor, glyphosate, glufosinate, and dicamba herbicides, wherein the HPPD inhibitor is selected from the group consisting of isoxaflutole, bicyclopyrone, mesotrione, sulcotrione, tembotrione, topramezone pyrasulfatole; and wherein the ALS inhibitor is selected from the group consisting of prosulfuron, primisulfuron, triasulfuron, bensulfuron, nicosulfuron, rimsulfuron, primisulfuron, thifensulfuron, foramsulfuron, chlorsulfuron, halosulfuron, imazaquin, imazapic, imazapyr, imazethapyr, imazamox, iodosulfuron, metsulfuron, mesosulfuron sulfosulfuron trifloxysulfuron tribenuron methyl, thiazopyr, diclosulam, cloransulam-methyl, flucarbazone, flumetsulam, thiencarbazone, and chlorimuron-ethyl.

7. The soybean plant of claim 2 , wherein the plant further comprise one or more additional regions encoding a polypeptide providing the plant with resistance or tolerance to one or more additional herbicides, one or more insects, or one or more fungal, bacterial and/or viral infections.

8. A method of weed control comprising applying a combination of at least two herbicides corresponding to the regions encoded by a polypeptide capable of providing the plant with resistance or tolerance to one or more additional herbicides on a field of transgenic soybean plants of claim 2 , wherein the transgenic soybean plants comprise herbicide tolerance event SYHT0H2, wherein a representative sample of seed comprising said event has been deposited under ATCC Accession No. PTA-11226, or further comprise one or more additional regions encoding a polypeptide capable of providing the plant with resistance or tolerance to one or more additional herbicides, or one or more insects, fungal, bacterial, and/or viral infections.

9. The method of claim 8 , wherein the application is (i) tank mix application, (ii) subsequent sprays, or (iii) pre-mix application.

10. A method of producing a soybean plant resistant to one or both of: an HPPD inhibitor and glufosinate, comprising the steps of: a. crossing a first soybean plant comprising event SYHT0H2, wherein a representative seed comprising said event has been deposited under ATCC Accession No. PTA-11226; and b. selecting at least a first progeny plant that comprises event SYHT0H2 and is resistant to one or both of the following: an HPPD inhibitor and glufosinate.

11. The method of claim 10 , further comprising the steps of:
a. selfing the first progeny plant to produce second generation progeny plants; and
b. selecting at least a first plant homozygous for event SYHT0H2.

12. A method of controlling weeds at a location comprising soybean plants and weeds, wherein the soybean plants comprise event SYHT0H2, wherein a representative seed comprising said event has been deposited under ATCC Accession No. PTA-11226, and wherein the method comprises applying to the location a weed controlling amount of an herbicidal composition comprising one or more HPPD inhibitors.

13. The method of claim 12 , wherein the one or more HPPD inhibitors is selected from the group consisting of bicyclopyrone, mesotrione, sulcotrione, topramezone, tembotrione, pyrosulfatole and isoxaflutole.

14. A method of controlling weeds at a location comprising soybean plants and weeds, said method comprising: a. growing soybean plants comprising event SYHT0H2, wherein a representative seed comprising said event has been deposited under ATCC Accession No. PTA-11226, or further comprising one or more additional regions encoding a polypeptide providing the plant with resistance or tolerance to one or more additional herbicides, the polypeptide being selected from the group consisting of glyphosate tolerant 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), glyphosate N-acetyl transferase (GAT), herbicide tolerant 4-hydroxypyruvyldioxgenase (HPPD), phosphinothricin actetyl transferase (PAT), cytochrome P450, glutathione S-transferase (GST), herbicide tolerant acetyl-COA-carboxylase (ACCase), herbicide tolerant acetolactate synthase (ALS), herbicide tolerant protoporphyrinogen oxidase (PPGO), bromoxynil nitrilase, herbicide tolerant phytoene desaturase, aryloxyalkanoate dioxygenase, and dicamba degrading enzymes; b. applying to the location a weed controlling amount of an herbicidal composition comprising one or more HPPD inhibitors, and c. further applying one or more additional fungicide, nematicide, insecticide, or herbicide.

15. The method of claim 14 , wherein the one or more additional herbicide from step (c) is an herbicide selected from the group consisting of glyphosate, glufosinate, fomesafen, saflufenacil, lactofen, aciflurofen, cafentrazone-ethyl fluthiacet, oxyfluorfen, flumiclorac sulfentrazone, flumioxazin, metolachlor, S-metolachlor, acetochlor, alachlor, pyroxasulfone, flufenacet, dimethenamid, dimethenamid-P, bromoxynil, bentazon, metribuzin, atrazine, terbuthylazine, diuron, Linuron, ametryn, fluazifop, clethodim, fenoxaprop, haloxyfop, quizalofop, bensulfuron, nicosulfuron, rimsulfuron, primisulfuron, thifensulfuron, foramsulfuron, chlorsulfuron, halosulfuron, imazaquin, imazapic, imazapyr imazethapyr, imazamox, iodosulfuron, metsulfuron, mesosulfuron sulfosulfuron trifloxysulfuron tribenuron methyl, thiazopyr, tebuthiuron, diclosulam, cloransulam-methyl, flucarbazone, flumetsulam amicarbazone, thiencarbazone, chlorimuron-ethyl, dicamba, 2,4-D, 2,4-DB, fluroxypyr, diflufenzopyr, tirclopyr, picloram, quinclorac, clopyralid and aminopyralid; and agrochemically acceptable salts thereof.

16. A method o

Assignees

• Hipskind John Daniel
• Burgin Kristina
• Jain Rakesh
• Terpstra Karolyn
• Sigareva Marina
• De Framond Annick Jeanne
• Breitinger Becky
• Kramer Vance Cary
• Gu Weining
• Syngenta Participations Ag

Inventors

• Hipskind John Daniel
• Burgin Kristina
• Jain Rakesh
• Terpstra Karolyn
• Sigareva Marina
• De Framond Annick Jeanne
• Breitinger Becky
• Kramer Vance Cary
• Gu Weining

Classifications

• C12Q1/6813

  Hybridisation assays · CPC title

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title

• C12N15/8286

  for insect resistance · CPC title

• C12N15/8283

  for virus resistance · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title