Flexible bioreactor and support structure system

Patent metadata

Field	Value
Publication number	US-10184099-B2
Application number	US-201615142811-A
Country	US
Kind code	B2
Filing date	Apr 29, 2016
Priority date	Mar 31, 2015
Publication date	Jan 22, 2019
Grant date	Jan 22, 2019

Abstract

Some embodiments include a system having a support structure configured to mechanically support a first bioreactor. The support structure can have a first frame and a second frame together being configured to mechanically support the first bioreactor in interposition between the first frame and the second frame. The first frame can maintain a first set point temperature of the first bioreactor through an exchange of thermal energy between the first frame and the first bioreactor when the first bioreactor is vitally supporting one or more first microorganisms and when the support structure is mechanically supporting the first bioreactor. Other embodiments of related systems and methods are also disclosed.

First claim

What is claimed is:

1. A system comprising:
a support structure configured to mechanically support a first bioreactor, the support structure comprising:
a first frame; and
a second frame, together with the first frame, being configured to mechanically support the first bioreactor in interposition between the first frame and the second frame;

wherein:
the first frame is configured to maintain a first set point temperature of the first bioreactor through an exchange of thermal energy between the first frame and the first bioreactor when the first bioreactor is vitally supporting one or more first microorganisms and when the support structure is mechanically supporting the first bioreactor; and
the second frame is configured to maintain the first set point temperature of the first bioreactor through an exchange of thermal energy between the second frame and the first bioreactor when the first bioreactor is vitally supporting the one or more first microorganisms and when the support structure is mechanically supporting the first bioreactor;
the first bioreactor comprises:
a first bioreactor cavity configured to contain the one or more first microorganisms and a first fluidic support medium; and
one or more first bioreactor walls at least partially enclosing the first bioreactor cavity, the one or more first bioreactor walls comprising at least one first bioreactor wall material and the at least one first bioreactor wall material being flexible;

the first frame comprises two or more discrete first frame rails, each first frame rail of the two or more first frame rails comprising a first frame rail conduit, wherein each first frame rail is vertical and parallel with the other first frame rails;
each first frame rail conduit of the two or more first frame rail conduits containing a temperature maintenance fluid configured to exchange thermal energy between the first bioreactor and the temperature maintenance fluid as it flows in an upward direction through each first frame rail conduit in order to maintain the first set point temperature of the first bioreactor when the first bioreactor is vitally supporting the one or more first microorganisms; and
the two or more first frame rails are operable to simultaneously mechanically support the first bioreactor and exchange thermal energy between the first bioreactor and the temperature maintenance fluid contained in the two or more first frame rail conduits;
the second frame of the support structure comprises two or more discrete second frame rails, each second frame rail of the two or more second frame rails comprising a second frame rail conduit, wherein each second frame rail is vertical and parallel with the other second frame rails;
each second frame rail conduit of the two or more second frame rail conduits containing the temperature maintenance fluid configured to exchange thermal energy between the first bioreactor and the temperature maintenance fluid as it flows in an upward direction through each second frame rail conduit in order to maintain the first set point temperature of the first bioreactor when the first bioreactor is vitally supporting the one or more first microorganisms;
a floor gap disposed beneath at least one of the first frame and the second frame between a floor and a lowest horizontal member of the at least one of the first frame and the second frame; and
at least part of the one or more first bioreactor walls of the first bioreactor bulges to dispose at least part of the one or more first bioreactor walls into the floor gap when the support structure is mechanically supporting the first bioreactor.

2. The system of claim 1 wherein:
the first bioreactor further comprises:
one or more bioreactor fittings in communication with the first bioreactor cavity, the one or more bioreactor fittings comprising at least one gas delivery fitting;
one or more gas delivery devices located within the first bioreactor cavity, the one or more gas delivery devices being operable to inject gas into the first bioreactor cavity to mix the one or more first microorganisms; and
one or more flexible tubes located within the first bioreactor cavity, the one or more flexible tubes comprising at least one gas delivery tube coupling the one or more gas delivery devices to the at least one gas delivery fitting.

3. The system of claim 2 wherein:
the two or more first frame rails and the two or more second frame rails are configured to receive the temperature maintenance fluid from only a single source of the temperature maintenance fluid in parallel in order to reduce a total path length of the temperature maintenance fluid upwardly through the two or more vertical first frame rails and through the two or more vertical second frame rails and in order to minimize temperature fluctuation.

4. The system of claim 1 wherein:
the support structure further comprises one or more adjustable coupling mechanisms coupling the first frame to the second frame; and
the second frame is configured to selectively move relative to the first frame in a direction orthogonal to the first frame when the one or more adjustable coupling mechanisms are adjusted.

5. The system of claim 2 further comprising:
at least one light source mechanically supported by the support structure and operable to illuminate the one or more first microorganisms when the first bioreactor is vitally supporting the one or more first microorganisms and when the support structure is mechanically supporting the first bioreactor.

6. The system of claim 5 wherein:
the support structure is further configured to mechanically support a second bioreactor;
the support structure comprises a third frame and a fourth frame together being configured to mechanically support the second bioreactor in interposition between the third frame and the fourth frame, the third frame further being configured to maintain a second set point temperature of the second bioreactor through an exchange of thermal energy between the third frame and the second bioreactor when the second bioreactor is vitally supporting one or more second microorganisms and when the support structure is mechanically supporting the second bioreactor;
and
the second bioreactor comprises:
a second bioreactor cavity configured to contain the one or more second microorganisms and a second fluidic support medium; and
one or more second bioreactor walls at least partially enclosing the second bioreactor cavity, the one or more second bioreactor walls comprising at least one second bioreactor wall material and the at least one second bioreactor wall material being flexible.

Assignees

• Heliae Dev Llc
• Heliae Dev Llc

Inventors

• Mazur Richard
• Cizek Luke
• Oelschlager Mason
• Mccarty Mason
• Tram Minh Duc
• Hayden Justin
• Bair Candyce

Classifications

• C12M23/34

  Internal compartments or partitions · CPC title

• C12M21/02 Primary

  Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12) · CPC title

• C12M23/40

  Manifolds; Distribution pieces (fluid transfer means B01L3/563) · CPC title

• C12M31/00

  Means for providing, directing, scattering or concentrating light (C12M41/06 takes precedence) · CPC title

• C12M29/06

  Nozzles; Sprayers; Spargers; Diffusers (per se B01F23/231, B01J19/26) · CPC title