Method for preparing a chitosan-based matrix comprising a fiber reinforcement member

Patent metadata

Field	Value
Publication number	US-10184032-B2
Application number	US-201615003975-A
Country	US
Kind code	B2
Filing date	Jan 22, 2016
Priority date	Feb 17, 2015
Publication date	Jan 22, 2019
Grant date	Jan 22, 2019

Abstract

The present invention relates to a method for preparing a chitosan-based matrix comprising the following steps:
a) two separate chitosan porous layers obtained from lyophilization of layers of one or more solutions of chitosan are provided, b) the chitosan porous layers of a) are neutralized and washed, c) a face of each chitosan porous layer is acidified with an acid, d) a fiber reinforcement member is provided and positioned between the two acidified faces of the two chitosan porous layers, e) the two chitosan porous layers and the fiber reinforcement member are let to dry to obtain a matrix having the fiber reinforcement member embedded therein. The invention also relates to an implant comprising a matrix obtained according to such a method.

First claim

What is claimed is:

1. A method for preparing a chitosan-based matrix comprising a fiber reinforcement member embedded in the matrix comprising:
a) providing one or more solutions including chitosan,
b) obtaining two separate chitosan porous layers from lyophilisation of the one or more solutions of chitosan,
c) neutralizing the chitosan porous layers of b),
d) washing the chitosan porous layers of c),
e) applying an acid to a face of each of the chitosan porous layers of d),
f) positioning a fiber reinforcement member between the two acidified faces of the two chitosan porous layers of e),
g) drying the two chitosan porous layers and the fiber reinforcement member of f) to obtain a matrix having the fiber reinforcement member embedded therein.

2. The method according to claim 1 , wherein providing one or more solutions including chitosan comprises a solution of chitosan in water, the chitosan including a concentration in the solution ranging from 0.1% to 10% by weight, relative to a total weight of the solution.

3. The method according to claim 1 , wherein providing one or more solutions including chitosan comprises a solution of chitosan in water, the chitosan including a concentration in the solution ranging from 0.5% to 3% by weight, relative to a total weight of the solution.

4. The method according to claim 1 , wherein providing one or more solutions including chitosan comprises a solution of chitosan in water, the chitosan including a concentration in the solution ranging from 0.8% to 1% by weight, relative to a total weight of the solution.

5. The method according to claim 1 , further comprising adjusting a pH of the one or more solutions in a) to a value ranging from 3 to 5.

6. The method according to claim 1 , further comprising adjusting a pH of the one or more solutions in a) to a value of 3.5.

7. The method according to claim 5 , wherein adjusting a pH of the one or more solutions in a) comprises adding acetic acid to the one or more of solutions.

8. The method according to claim 1 , wherein applying an acid according to e) comprises spraying a solution of the acid on the face.

9. The method according to claim 1 , wherein the acid according to e) is selected from the group consisting of acetic acid, hydrochloric acid, citric acid, sulphuric acid, lactic acid, and mixtures thereof.

10. The method according to claim 1 , wherein the acid according to e) comprises acetic acid.

11. The method according to claim 10 , wherein applying the acid according to e) comprises spraying a solution of acetic acid at 0.1 M on the face of each of the chitosan porous layers.

12. The method according to claim 11 , wherein the spraying is repeated twice.

13. The method of claim 1 , further comprising impregnating the fiber reinforcement member with a solution of an acid before being positioned between the two acidified faces of the two chitosan porous layers of e).

14. The method according to claim 13 , wherein the solution of the acid comprises an acid selected from the group consisting of acetic acid, hydrochloric acid, citric acid, sulphuric acid, lactic acid, and mixtures thereof.

15. The method according to claim 13 , wherein the solution of the acid comprises acetic acid.

16. The method according to claim 15 , wherein the solution of the acid comprises a solution of acetic acid at 0.1 M.

17. The method according to claim 1 , wherein the chitosan porous layers and the fiber reinforcement member have substantially bidimensional structures, and an area of the fiber reinforcement member is smaller than an area of each chitosan porous layer.

18. The method according to claim 1 , wherein the fiber reinforcement member is a porous mesh.

19. The method according to claim 1 , further comprising applying a pressure on the chitosan porous layers in a direction of the fiber reinforcement member during and/or after the positioning a fiber reinforcement member between the two acidified faces of the two chitosan porous layers of e).

20. The method according to claim 1 , wherein neutralizing the chitosan porous layers of b) comprises immersing the chitosan porous layers of a) in a basic solution.

21. The method according to claim 20 , wherein the basic solution comprises NaOH or NH 4 OH in water or an alcohol.

22. The method according to claim 1 , wherein washing the chitosan porous layers of c) comprises dipping the chitosan porous layers of c) in one or more baths of sterile water until a washing water with a pH close to 7 is obtained.

23. The method according to claim 1 , wherein applying an acid to a face of each of the chitosan porous layers of d) further comprises obtaining a superficial layer on the face of each of the chitosan porous layers so as to cause a resolubilization of the superficial layer of each face.

24. The method according to claim 23 , wherein the superficial layer comprises a thickness up to 5% of a total thickness of each chitosan porous layer.

Assignees

• Sofradim Production

Inventors

• Serrero Aurelie
• Desorme Mylene

Classifications

• C08J2305/08

  Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof · CPC title

• C08B37/003

  Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof · CPC title

• A61L27/56

  Porous materials, {e.g. foams or sponges} · CPC title

• C08J5/046 Primary

  with synthetic macromolecular fibrous material · CPC title

• A61L27/20

  Polysaccharides · CPC title