Use of single dendritic wedge cell penetrating peptides to facilitate cellular delivery of nanoparticles and nanoparticles carrying cargos

Patent metadata

Field	Value
Publication number	US-10183080-B2
Application number	US-201715694114-A
Country	US
Kind code	B2
Filing date	Sep 1, 2017
Priority date	Sep 2, 2016
Publication date	Jan 22, 2019
Grant date	Jan 22, 2019

Abstract

Nanoparticles (and optionally a cargo such as a drug) can be delivered to cells by attaching just a single dendritic peptide to the nanoparticle. The dendritic peptide includes a polyhisitidine motif and a hinge and a spacer connecting the polyhistidine to a lysine-based dendritic wedge displaying at least two copies of a cell-penetrating peptide motif.

First claim

What is claimed is:

1. A method of delivering a nanoparticle to a cell comprising:
contacting a living cell with a nanoparticle bound to a single dendritic peptide thereby causing entry of the nanoparticle into the cell,
wherein the dendritic peptide comprises a polyhisitidine motif and a hinge and a spacer connecting the polyhistidine to a lysine-based dendritic wedge displaying at least two copies of the peptide sequence RRRRRRRRRFG (SEQ ID No: 2).

2. The method of claim 1 , wherein the nanoparticle is also bound to a cargo which is delivered to the cell with the nanoparticle.

3. A method of delivering a nanoparticle to a cell comprising:
contacting a living cell with a nanoparticle bound to a single dendritic peptide thereby causing entry of the nanoparticle into the cell,
wherein the dendritic peptide comprises a hexahistidine motif connected to a lysine-based dendritic wedge displaying at least two copies of the peptide sequence RRRRRRRRRFG (SEQ ID No: 2) via a hinge and spacer comprising six prolines and two glycines.

4. The method of claim 3 , wherein the nanoparticle is also bound to a cargo which is delivered to the cell with the nanoparticle.

5. A method of delivering a nanoparticle to a cell comprising:
contacting a living cell with a nanoparticle bound to a single dendritic peptide thereby causing entry of the nanoparticle into the cell
wherein the dendritic peptide comprises a polyhisitidine motif and a hinge and a spacer connecting the polyhistidine to a lysine-based dendritic wedge displaying at least two copies of the peptide sequence RRRRRRRRRFG (SEQ ID No: 2); and
wherein the nanoparticle is free of any other cell-penetrating peptides.

6. The method of claim 5 , wherein the nanoparticle is also bound to a cargo which is delivered to the cell with the nanoparticle.

Assignees

• Us Gov Sec Navy

Inventors

• Medintz Igor L
• Delehanty James B
• Breger Joyce
• Muttenthaler Markus
• Dawson Philip E

Classifications

• A61K38/08 Primary

  Peptides having 5 to 11 amino acids {(A61K38/043 - A61K38/046 take precedence)} · CPC title

• A61K47/6923

  the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb · CPC title

• A61K47/645

  Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT · CPC title

• A61K47/52

  the modifying agent being an inorganic compound, e.g. an inorganic ion that is complexed with the active ingredient · CPC title

• A61K47/62

  the modifying agent being a protein, peptide or polyamino acid · CPC title