Plant-adventitious-embryo induction method, plant restoration method, and plant reproduction method

Patent metadata

Field	Value
Publication number	US-10172307-B2
Application number	US-201314422339-A
Country	US
Kind code	B2
Filing date	Sep 4, 2013
Priority date	Sep 5, 2012
Publication date	Jan 8, 2019
Grant date	Jan 8, 2019

Abstract

The present invention provides a method of regenerating a plant, which allows stable regeneration of plants from calli; and a method of reproducing a plant, which allows stable reproduction of plants without being affected by weather, seasons or other factors. The present invention relates to a method of regenerating a plant, including a step of inducing adventitious embryos from calli; and a method of reproducing a plant, including a step of inducing adventitious embryos from calli.

First claim

The invention claimed is:

1. A method of regenerating a plant, comprising: a step of inducing adventitious embryos from calli in a regeneration induction medium that contains naphthaleneacetic acid and benzyladenine, wherein said plant is a plant of the genus Sonchus , and wherein a concentration of the naphthaleneacetic acid is 1×10 −2 to 0.05 mg/L, and a concentration of the benzyladenine is 0.8 to 1.2 mg/L.

2. The method of regenerating the plant according to claim 1 ,
wherein the adventitious embryo induction is followed by forming shoots.

3. The method of regenerating the plant according to claim 1 , comprising:
a regeneration induction step of culturing the calli in a regeneration induction medium that contains naphthaleneacetic acid, benzyladenine, and a carbon source to form the adventitious embryos and shoots; and
a rooting step of culturing the shoots in a rooting medium to root the shoots.

4. The method of regenerating the plant according to claim 1 , comprising:
a regeneration induction step of culturing the calli in a regeneration induction medium that contains naphthaleneacetic acid, benzyladenine, and a carbon source to form the adventitious embryos and shoots;
an elongation step of culturing the formed shoots in an elongation medium to elongate the shoots; and
a rooting step of culturing the elongated shoots in a rooting medium to root the shoots.

5. The method of regenerating the plant according to claim 4 ,
wherein the elongation medium and the rooting medium are free of plant growth hormones.

6. The method of regenerating the plant according to claim 1 ,
wherein the plant is Sonchus oleraceus.

7. A method of reproducing a plant, comprising: a step of inducing adventitious embryos from calli in a regeneration induction medium that contains naphthaleneacetic acid and benzyladenine, wherein said plant is plant of the genus Sonchus , and wherein a concentration of the naphthaleneacetic acid is 1×10 −2 to 0.05 mg/L, and a concentration of the benzyladenine is 0.8 to 1.2 mg/L.

8. The method of reproducing the plant according to claim 7 , comprising a step of inducing the calli from tissue sections of the plant.

9. The method of reproducing the plant according to claim 7 ,
wherein the adventitious embryo induction is followed by forming shoots.

10. The method of reproducing the plant according to claim 7 , comprising:
an induction step of culturing tissue sections of the plant in an induction medium that contains a plant growth hormone and a carbon source to induce the calli;
a regeneration induction step of culturing the calli in a regeneration induction medium that contains naphthaleneacetic acid, benzyladenine, and a carbon source to form the adventitious embryos and shoots; and
a rooting step of culturing the shoots in a rooting medium to root the shoots.

11. The method of reproducing the plant according to claim 7 , comprising:
an induction step of culturing tissue sections of the plant in an induction medium that contains a plant growth hormone and a carbon source to induce the calli;
a regeneration induction step of culturing the calli in a regeneration induction medium that contains naphthaleneacetic acid, benzyladenine, and a carbon source to form the adventitious embryos and shoots;
an elongation step of culturing the formed shoots in an elongation medium to elongate the shoots; and
a rooting step of culturing the elongated shoots in a rooting medium to root the shoots.

12. The method of plant according to claim 7 ,
wherein the plant is Sonchus oleraceus.

13. A method of inducing adventitious embryos of a plant of the genus Sonchus , comprising: culturing calli a medium that contains naphthaleneacetic acid, benzyladenine, and a carbon source to induce the adventitious embryos, wherein the medium has a concentration of the naphthaleneacetic acid of 1×10 −2 to 0.05 mg/L, and a concentration of the benzyladenine of 0.8 to 1.2 mg/L.

14. The method of inducing adventitious embryos according to claim 13 , wherein the medium has a solidifying agent concentration of 0.1 to 2% by mass.

Assignees

• Sumitomo Rubber Ind

Inventors

• OKADA Akari
• INOUE Yukino
• Kuroda Satoshi

Classifications

• A01H4/008

  Methods for regeneration to complete plants · CPC title

• C12N5/04

  Plant cells or tissues {(culture media C12N5/0025)} · CPC title

• A01H6/14

  Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce · CPC title

• A01H4/005 Primary

  Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques · CPC title

• A01H4/001

  Culture apparatus for tissue culture · CPC title