Herbicide resistance genes

Patent metadata

Field	Value
Publication number	US-10167483-B2
Application number	US-201414491197-A
Country	US
Kind code	B2
Filing date	Sep 19, 2014
Priority date	Oct 28, 2005
Publication date	Jan 1, 2019
Grant date	Jan 1, 2019

Abstract

The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

First claim

We claim:

1. An expression cassette for expression in a plant cell, comprising a polynucleotide operably linked to a heterologous plant promoter or a plant virus promoter, wherein said polynucleotide encodes a protein that catalyzes degradation of phenoxy auxin and pyridyloxy auxin herbicides, wherein the polynucleotide that encodes said protein comprises SEQ ID NO:3.

2. The expression cassette of claim 1 , wherein said polynucleotide comprises plant codons for expression in said plant cell.

3. The expression cassette of claim 1 , wherein said plant promoter or said plant virus promoter is selected from a cassava vein mosaic virus promoter, a CaMV 35S promoter, a Figwort Mosaic Virus promoter, a rice actin promoter, a phaseolin promoter, an Arabidopsis thaliana Ubiquitin 10 promoter, a maize ubiquitin promoter, an Arabidopsis thaliana Act2 promoter, an Arabidopsis thaliana Ubiquitin 11 promoter, and an Arabidopsis thaliana Ubiquitin 3 promoter.

4. A method of controlling herbicide resistance in weeds in a field, wherein said method comprises applying to said weeds at least one herbicide selected from the group consisting of a pyridyloxy auxin herbicide and a phenoxy auxin herbicide, said field comprising a plurality of crop plants comprising the expression cassette of claim 1 .

5. A method of selecting at least one transgenic plant that is resistant or tolerant to both phenoxy auxin and pyridyloxy auxin herbicides, said method comprising applying a herbicidically effective amount of an aryloxyalkanoate herbicide to a plurality of plants in a field, wherein said plurality of plants in said field comprises at least one plant that is stably transformed with the expression cassette of claim 1 and is able to grow in said effective amount of the aryloxyalkanoate herbicide, and identifying said plant that is stably transformed with said expression cassette.

6. The method of claim 4 , wherein said plurality of crop plants are dicots.

7. The method of claim 6 , wherein said dicots are selected from the group consisting of soybean plants, cotton plants, canola plants, sugar beet plants, tomato plants, and sunflower plants.

8. The method of claim 4 , wherein said method comprises applying to said weeds at least one herbicide selected from the group consisting of a pyridyloxy auxin herbicide and a phenoxy auxin herbicide, and wherein said pyridyloxy auxin herbicide comprises one or more pyridyloxyacetate herbicides.

9. The method of claim 8 , wherein said pyridyloxyacetate herbicide(s) is selected from the group consisting of triclopyr and fluroxypyr.

10. The method of claim 4 , wherein said applying step comprises applying said herbicide to said crop plants within 14 days of planting a seed in said field, wherein said seed comprises an expression cassette comprising a polynucleotide operably linked to a heterologous plant promoter or a plant virus promoter, wherein said polynucleotide encodes a protein that catalyzes degradation of phenoxy auxin and pyridyloxy auxin herbicides, wherein the polynucleotide that encodes said protein comprises SEQ ID NO:3.

11. The method of claim 4 , wherein said plurality of crop plants further comprises a second polynucleotide encoding a second protein that confers said crop plants with resistance to at least one other herbicide, wherein said second polynucleotide is heterologous, and said method further comprises applying said at least one other herbicide to least a portion of said field.

12. The method of claim 11 , wherein said herbicides are applied sequentially or concurrently.

13. The method of claim 11 , wherein said herbicides are applied from a tank mix.

14. The method of claim 11 , wherein said at least one other herbicide is selected from the group consisting of acetochlor, acifluorfen, alloxydim, amidosulfuron, aminopyralid, atrazine, beflubutamid, bispyribac, butafenacil, cafenstrole, carfentrazone, chlorimuron, chlorotoluron, cinidon-ethyl, clethodim, clodinafop, clomazone, cloproxydim, clopyralid, cloransulam, cyanazine, cyclosulfamuron, cycloxydim, cyhalofop, daimuron, dicamba, diclofop, diclosulam, diflufenican, dimethenamid, diquat, dithiopyr, diuron, ethalfluralin, fenoxaprop, flazasulfuron, florasulam, fluazifop, flucarbazone, flufenacet, flufenican, flufenpyr, flumetsulam, flumiclorac, flumioxazin, fluroxypyr, fluthiacet, fomesafen, foramsulfuron, glufosinate, glyphosate, halosafen, halosulfuron, haloxyfop, imazamethabenz, imazamox, imazapic, imazapyr, imazaquin, imazethapyr, imazosulfuron, iodosulfuron, ioxynil, isoxaben, isoxaflutole, lactofen, linuron, mefenacet, mefluidide, mesosulfuron, mesotrione, metamifop, metazachlor, metosulam, metribuzin, MSMA, napropamide, nicosulfuron, norflurazon, oryzalin, oxadiazon, oxyfluorfen, paraquat, pebulate, pendimethalin, penoxsulam, picloram, picolinafen, pinoxaden, primisulfuron, profoxydim, propanil, pyraflufen, pyrazosulfuron, pyribenzoxim, pyriminobac, pyrithiobac, pyroxasulfone, pyroxsulam, quinclorac, quinmerac, quizalofop, rimsulfuron, sethoxydim, simazine, sulcotrione, sulfentrazone, sulfometuron, tefuryltrione, tembotrione, tepraloxydim, terbacil, thiazopyr, thidiazuron, thiencarbazone, thifensulfuron, thiobencarb, topramezone, tralkoxydim, triasulfuron, tribenuron, triclopyr, trifloxysulfuron, trifluralin, triflusulfuron, and tritosulfuron.

15. The method of claim 4 , wherein said weeds are glyphosate-resistant.

16. The method of claim 15 , wherein said crop plants are glyphosate tolerant crop plants.

17. The method of claim 4 , wherein said herbicide is the phenoxy auxin herbicide.

18. The method of claim 17 , wherein said phenoxy auxin is selected from the group consisting of MCPA and a 2,4-D herbicide.

19. A method of controlling volunteer plants in a field, wherein said method comprises applying to said volunteer plants at least one herbicide selected from the group consisting of a pyridyloxy auxin herbicide and a phenoxy auxin herbicide, said field comprising a plurality of transgenic crop plants comprising the expression cassette of claim 1 .

20. The method of claim 19 wherein said volunteer plants are glyphosate-resistant and of a different species than said transgenic crop plants.

21. A polynucleotide operably linked to a heterologous plant promoter or a plant virus promoter, wherein the polynucleotide encodes a protein having aryloxyalkanoate dioxygenase activity, wherein the protein enzymatically degrades phenoxy auxin and pyridyloxy auxin herbicides, and wherein the polynucleotide that encodes said protein comprises SEQ ID NO:3.

22. A polynucleotide optimized for expression in a plant wherein said polynucleotide is operably linked to a heterologous plant promoter or a plant virus promoter, wherein the polynucleotide encodes a protein having aryloxyalkanoate dioxygenase activity, wherein the protein enzymatically degrades phenoxy auxin and pyridyloxy auxin herbicides, and wherein the polynucleotide that encodes said protein has SEQ ID NO:3.

23. The polynucleotide of claim 22 wherein said polynucleotide is optimized for expression in a dicotyledonous plant or a monocotyledonous plant.

24. An isolated polynucleotide that is operably linked to a heterologous plant promoter or a plant virus promoter, wherein the polynucleotide encodes a protein that enzymatically degrades phenoxy auxin and pyridyloxy auxin herbicides, and wherein the polynucleotide that encodes said protein comprises SEQ ID NO:3.

25. The polynucleotide of claim 24 wh

Assignees

• Dow Agrosciences Llc

Inventors

• Wright Terry R
• Lira Justin M
• Walsh Terence Anthony
• Merlo Donald
• Samuel Jayakumar Pon
• Lin Gaofeng

Classifications

• C12N9/0069

  acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13) · CPC title

• C12N15/8275

  Glyphosate · CPC title

• C12N15/8274 Primary

  for herbicide resistance · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12Y113/11

  with incorporation of two atoms of oxygen (1.13.11) · CPC title