Modified recombinant variable lymphocyte receptors (VLR)

Patent metadata

Field	Value
Publication number	US-10167330-B2
Application number	US-201515308535-A
Country	US
Kind code	B2
Filing date	Apr 30, 2015
Priority date	May 2, 2014
Publication date	Jan 1, 2019
Grant date	Jan 1, 2019

Abstract

This disclosure relates to variable lymphocyte receptors (VLRs) modifications such as humanized sequences and polypeptides comprising such sequences that specifically bind a target molecule and uses related thereto. In certain embodiments, the disclosure relates to recombinant polypeptide VLRs disclosed herein and variants thereof. In certain embodiments, this disclosure relates to treating or preventing a disease or condition comprising administering an effective amount of a recombinant polypeptide or variant disclosed herein to a subject in need thereof.

First claim

What we claim:

1. A recombinant polypeptide comprising an amino acid sequence XLXLXXNKLQTIAKGTFSPLRAIQXMXLXX (SEQ ID NO: 38) wherein X at each position is individually and independently any amino acid provided that the polypeptide does not contain at least one of the following wild-type Slit2-D2 sequences selected from LLSLYD (SEQ ID NO: 16), and TMHLAQ (SEQ ID NO: 17).

2. The recombinant polypeptide of claim 1 further comprising a human Fc sequence.

3. The recombinant polypeptide of claim 1 comprising an amino acid sequence DLHNLNY L D L NN NKLQTIAKGTFSPLRAIQ H M W L YQNPFICDC (SEQ ID NO: 71).

4. The recombinant polypeptide of claim 1 comprising an amino acid sequence CPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNN QISELAPDAFQGLRSLNSLDLGGNKITELPKSLFEGLFSLQLLLLNANKINXLRVDAFQDL HNLNLLSLDDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLSIAMRWDGK AVNDPDSARCTSPRRLANKRIGQIKSKKFRC (SEQ ID NO: 24).

5. The recombinant polypeptide of claim 1 comprising an amino acid sequence CPAACTCSNNTVDCRSKGLTEIPTNLPETITIIYLHDNTIKVIPPGAFSPYKKLREIYLGSNQ ISELAPDAFQGLRSLNVLDLGTNKITELPKSLFEGLFSLQELFLCCNKINCLRVDAFQDLH NLNHLALDQNKLQTIAKGTFSPLRAIQHMYLFGNPFICDCHLKWLADYLHIAMIMDGK AVNDPDSARCTSPRRLANKRIGQIKSKKFRC (SEQ ID NO: 1).

6. The recombinant polypeptide of claim 1 , wherein an X is the amino acid tyrosine.

7. The recombinant polypeptide of claim 1 , wherein an X is the amino acid selected from phenylalanine (Phe), tryptophan (Trp) and histidine (His).

8. The recombinant polypeptide of claim 1 , wherein an X is the amino acid selected from asparagine (Asn) and aspartic acid (Asp).

Assignees

• Univ Emory

Inventors

• Herrin Brantley R
• Cooper Max Dale
• Ehrhardt Rudolf

Classifications

• C07K14/7051 Primary

  T-cell receptor (TcR)-CD3 complex · CPC title

• C07K2319/30

  Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto · CPC title

• C12N15/00

  Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor (mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00) · CPC title

• C07K14/461

  from fish · CPC title