Nanopatterned biosensor electrode for enhanced sensor signal and sensitivity

Patent metadata

Field	Value
Publication number	US-10161898-B2
Application number	US-201715419524-A
Country	US
Kind code	B2
Filing date	Jan 30, 2017
Priority date	Jan 30, 2017
Publication date	Dec 25, 2018
Grant date	Dec 25, 2018

Abstract

Methods for forming an electrode structure, which can be used as a biosensor, are provided in which the electrode structure has non-random topography located on one surface of an electrode base. In some embodiments, an electrode structure is obtained that contains no interface between the non-random topography of the electrode structure and the electrode base of the electrode structure. In other embodiments, electrode structures are obtained that have an interface between the non-random topography of the electrode structure and the electrode base of the electrode structure.

First claim

What is claimed is:

1. A method of forming an electrode structure, said method comprising:
providing a mold having a pattern that comprises both an electrode base shape and a nanotopography shape;
forming a metallic seed layer on exposed surfaces of said mold having said electrode base shape and said nanotopography shape;
electrodepositing a conductive metal-containing material on said metallic seed layer and within said mold to provide an electrode structure comprising said conductive metal-containing material and having said electrode base shape and said nanotopography shape;
removing said mold from said electrode structure; and
attaching a biological functionalization material to said electrode structure.

2. The method of claim 1 , wherein said providing said mold comprises:
first patterning a substrate to provide said electrode base shape in said substrate; and
second patterning said substrate containing said electrode base shape to provide said nanotopography shape into said substrate.

3. The method of claim 1 , wherein said metallic seed layer and said conductive metal-containing material comprise a same metal or metal alloy.

4. The method of claim 1 , further comprising: removing excess conductive metal-containing material formed atop said mold.

5. The method of claim 1 , wherein said removing said mold comprises contacting said mold with a chemical wet etchant.

6. The method of claim 1 , wherein said biological functionalization material is composed of an oligonucleotide, a nucleic acid, a peptide, a ligand, a protein, an enzyme, or any other material apt to bind with a complementary target biomolecule.

7. The method of claim 6 , wherein said biological functionalization material is composed of glucose oxidase or glucose dehydrogenase.

8. The method of claim 1 , wherein no interface is present between said electrode base shape and said nanotopography shape.

Assignees

• IBM

Inventors

• Kinser Emily R

Classifications

• C25D5/48

  After-treatment of electroplated surfaces · CPC title

• C12Q1/006

  for glucose · CPC title

• C25D7/123

  Semiconductors first coated with a seed layer or a conductive layer · CPC title

• G01N27/3271 Primary

  Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood (amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001) · CPC title

• C25D1/006 Primary

  Nanostructures, e.g. using aluminium anodic oxidation templates [AAO] · CPC title