Pluripotent tissue harvester and methods of manufacture thereof

Patent metadata

Field	Value
Publication number	US-10160946-B2
Application number	US-201414917035-A
Country	US
Kind code	B2
Filing date	Sep 12, 2014
Priority date	Sep 13, 2013
Publication date	Dec 25, 2018
Grant date	Dec 25, 2018

Abstract

Disclosed herein is a tissue harvester comprising a substrate having an airfoil shape; and a textured surface disposed upon the substrate, where the textured surface comprises a spatial array of nanometer or micrometer sized pillars of varying cross-sections. Disclosed herein too is method comprising disposing upon a substrate a textured surface; where the substrate has an airfoil shape and where the textured surface where the textured surface comprises nanometer or micrometer sized pillars; contacting the tissue harvester with biological cells whose proliferation under different conditions is desired; and disposing the tissue harvester in a flow field such that cells disposed on the tissue harvester at different locations may experience different flow fields.

First claim

What is claimed is:

1. A tissue harvester comprising: a substrate having an airfoil shape; and a textured surface disposed upon the substrate, where the textured surface comprises a plurality of patterns; where the tissue harvester is disposed in a flow field along with cells that are to be generated on the tissue harvester; and where the textured surface is formed by a block copolymer that has one of its domains etched, leaving behind the other domain in the form of pillars to texture the surface.

2. The tissue harvester of claim 1 , where the substrate and the textured surface are monolithic.

3. The tissue harvester of claim 1 , where the textured surface is bonded to the substrate.

4. The tissue harvester of claim 1 , where the substrate and/or the textured surface comprise a polymer, a ceramic or a metal.

5. The tissue harvester of claim 1 , where the block copolymer is poly(styrene-b-vinyl pyridine), poly(styrene-b-butadiene), poly(styrene-b-isoprene), poly(styrene-b-methyl methacrylate), poly(styrene-b-alkenyl aromatics), poly(isoprene-b-ethylene oxide), poly(styrene-b-(ethylene-propylene)), poly(ethylene oxide-b-caprolactone), poly(butadiene-b-ethylene oxide), poly(styrene-b-t-butyl (meth)acrylate), poly(methyl methacrylate-b-t-butyl methacrylate), poly(ethylene oxide-b-propylene oxide), poly(styrene-b-tetrahydrofuran), poly(styrene-b-isoprene-b-ethylene oxide), poly(styrene-b-dimethylsiloxane), poly(methyl methacrylate-b-dimethylsiloxane), or a combination comprising at least one of the foregoing block copolymers.

6. The tissue harvester of claim 1 , where the pillars have a diameter of less than 100 nanometers.

7. The tissue harvester of claim 1 , where the pillars have a diameter of less than 100 micrometers.

8. The tissue harvester of claim 1 , where the textured surface comprises objects having square, rectangular, ellipsoidal, triangular, circular, or polygonal cross-sections.

9. A method comprising: disposing upon a substrate a textured surface; where the substrate has an airfoil shape and where the textured surface where the textured surface comprises nanometer or micrometer sized pillars; contacting the tissue harvester with biological cells whose proliferation under different conditions is desired; and disposing the tissue harvester in a flow field such that cells disposed on the tissue harvester at different locations may experience different flow fields; where the flow field is a pulsating flow field.

10. The method of claim 9 , where the cells adhere to the tissue harvester.

11. The method of claim 10 , where the cells proliferate on the tissue harvester.

12. The method of claim 11 , further comprising removing the proliferated cells from the tissue harvester.

13. The method of claim 9 , where the cells experience a gradient in flow fields from laminar to turbulent.

14. A tissue harvester comprising: a substrate having an airfoil shape; and a textured surface disposed upon the substrate, where the textured surface comprises a plurality of patterns; where the tissue harvester is disposed in a flow field along with cells that are to be generated on the tissue harvester; and where the patterns are projected into the surface of the substrate.

Assignees

• Univ Florida

Inventors

• Hanna Mina-Elraheb Saad
• Nino Juan Claudio

Classifications

• C12M35/04

  Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli · CPC title

• C12M23/02

  Form or structure of the vessel (large containers B65D88/00) · CPC title

• C12M25/00

  Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings · CPC title

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12M33/00 Primary

  Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus (chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00) · CPC title